Spatial patterns of bacterial richness and evenness in the NW Mediterranean Sea explored by pyrosequencing of the 16S rRNA
13 pages, 8 figures, 2 tables
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| Main Authors: | , , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
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Inter Research
2010-12-30
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| Subjects: | Bacterial diversity, Pyrosequencing, Blanes Bay Microbial Observatory, |
| Online Access: | http://hdl.handle.net/10261/80384 |
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dig-icm-es-10261-803842020-12-09T16:17:56Z Spatial patterns of bacterial richness and evenness in the NW Mediterranean Sea explored by pyrosequencing of the 16S rRNA Pommier, Thomas Neal, P. R. Gasol, Josep M. Coll-Lladó, Montserrat Acinas, Silvia G. Pedrós-Alió, Carlos Bacterial diversity Pyrosequencing Blanes Bay Microbial Observatory 13 pages, 8 figures, 2 tables Due to analytical limitations, patterns of richness and evenness of microbes are scarce in the current literature. The newest and powerful pyrosequencing technology may solve this issue by sampling thousands of sequences from the same community. We conducted a study of diversity along a horizontal transect (ca. 120 km) and a depth profile (surface to bottom at ca. 2000 m) in the northwestern Mediterranean Sea, using this technology on the V6 region of the 16S rDNA gene and analyzed patterns of richness and evenness of marine free-living bacterial communities. A total of 201 605 tag sequences were obtained from the 10 samples considered and clustered according to their similarity in 1200 operational taxonomic units (OTUs) per sample on average. We found a parallel decrease in richness and evenness from coast to offshore and from bottom to surface. We also observed a predominance of a few OTUs in each sample, while ca. 50% of all OTUs were found as singletons, which indicated that the community structures differed dramatically between sites despite the relative proximity and the physical connectivity between the samples. Despite these differences, using the 300 most abundant OTUs only was sufficient to obtain the same clustering of samples as with the complete dataset. Finally, both richness and evenness were negatively correlated with bacterial biomass and heterotrophic production The Modivus cruise was supported by the Spanish MICINN project MODIVUS (CTM2005-04795/ MAR). [...] Additional work was supported by a Spanish MICINN grant GEMMA (CTM2007-63753-C02-01/MAR). This is a contribution to the International Census of Marine Microbes (ICoMM) Peer Reviewed 2013-07-31T07:37:52Z 2013-07-31T07:37:52Z 2010-12-30 2013-07-31T07:37:53Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.3354/ame01484 issn: 0948-3055 e-issn: 1616-1564 Aquatic Microbial Ecology 61(3): 221-233 (2010) http://hdl.handle.net/10261/80384 10.3354/ame01484 en https://doi.org/10.3354/ame01484 open Inter Research |
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biblioteca |
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Europa del Sur |
| libraryname |
Biblioteca del ICM España |
| language |
English |
| topic |
Bacterial diversity Pyrosequencing Blanes Bay Microbial Observatory Bacterial diversity Pyrosequencing Blanes Bay Microbial Observatory |
| spellingShingle |
Bacterial diversity Pyrosequencing Blanes Bay Microbial Observatory Bacterial diversity Pyrosequencing Blanes Bay Microbial Observatory Pommier, Thomas Neal, P. R. Gasol, Josep M. Coll-Lladó, Montserrat Acinas, Silvia G. Pedrós-Alió, Carlos Spatial patterns of bacterial richness and evenness in the NW Mediterranean Sea explored by pyrosequencing of the 16S rRNA |
| description |
13 pages, 8 figures, 2 tables |
| format |
artículo |
| topic_facet |
Bacterial diversity Pyrosequencing Blanes Bay Microbial Observatory |
| author |
Pommier, Thomas Neal, P. R. Gasol, Josep M. Coll-Lladó, Montserrat Acinas, Silvia G. Pedrós-Alió, Carlos |
| author_facet |
Pommier, Thomas Neal, P. R. Gasol, Josep M. Coll-Lladó, Montserrat Acinas, Silvia G. Pedrós-Alió, Carlos |
| author_sort |
Pommier, Thomas |
| title |
Spatial patterns of bacterial richness and evenness in the NW Mediterranean Sea explored by pyrosequencing of the 16S rRNA |
| title_short |
Spatial patterns of bacterial richness and evenness in the NW Mediterranean Sea explored by pyrosequencing of the 16S rRNA |
| title_full |
Spatial patterns of bacterial richness and evenness in the NW Mediterranean Sea explored by pyrosequencing of the 16S rRNA |
| title_fullStr |
Spatial patterns of bacterial richness and evenness in the NW Mediterranean Sea explored by pyrosequencing of the 16S rRNA |
| title_full_unstemmed |
Spatial patterns of bacterial richness and evenness in the NW Mediterranean Sea explored by pyrosequencing of the 16S rRNA |
| title_sort |
spatial patterns of bacterial richness and evenness in the nw mediterranean sea explored by pyrosequencing of the 16s rrna |
| publisher |
Inter Research |
| publishDate |
2010-12-30 |
| url |
http://hdl.handle.net/10261/80384 |
| work_keys_str_mv |
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