DNA Barcoding the Medusozoa using mtCOI

DNA Barcoding the Medusozoa using mtCOI

Special issue Species Diversity of Marine Zooplankton.-- 9 pages, 4 figures, 3 tables

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Main Authors: Ortman, Brian D., Bucklin, Ann, Pagès, Francesc, Youngbluth, Marsh
Format: artículo biblioteca
Language:English
Published: Pergamon Press 2010-12
Subjects:Medusozoa, DNA barcoding, mtCOI, Molecular identification, Gelatinous zooplankton,
Online Access:http://hdl.handle.net/10261/78532
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spelling dig-icm-es-10261-785322020-12-09T16:17:55Z DNA Barcoding the Medusozoa using mtCOI Ortman, Brian D. Bucklin, Ann Pagès, Francesc Youngbluth, Marsh Medusozoa DNA barcoding mtCOI Molecular identification Gelatinous zooplankton Special issue Species Diversity of Marine Zooplankton.-- 9 pages, 4 figures, 3 tables The Medusozoa are a clade within the Cnidaria comprising the classes Hydrozoa, Scyphozoa, and Cubozoa. Identification of medusozoan species is challenging, even for taxonomic experts, due to their fragile forms and complex, morphologically-distinct life history stages. In this study 231 sequences for a portion of the mitochondrial Cytochrome Oxidase I (mtCOI) gene were obtained from 95 species of Medusozoans including; 84 hydrozoans (61 siphonophores, eight anthomedusae, four leptomedusae, seven trachymedusae, and four narcomedusae), 10 scyphozoans (three coronatae, four semaeostomae, two rhizostomae, and one stauromedusae), and one cubozoan. This region of mtCOI has been used as a DNA barcode (i.e., a molecular character for species recognition and discrimination) for a diverse array of taxa, including some Cnidaria. Kimura 2-parameter (K2P) genetic distances between sequence variants within species ranged from 0 to 0.057 (mean 0.013). Within the 13 genera for which multiple species were available, K2P distance between congeneric species ranged from 0.056 to 0.381. A cluster diagram generated by Neighbor Joining (NJ) using K2P distances reliably clustered all barcodes of the same species with ≥99% bootstrap support, ensuring accurate identification of species. Intra- and inter-specific variation of the mtCOI gene for the Medusozoa are appropriate for this gene to be used as a DNA barcode for species-level identification, but not for phylogenetic analysis or taxonomic classification of unknown sequences at higher taxonomic levels. This study provides a set of molecular tools that can be used to address questions of speciation, biodiversity, life-history, and population boundaries in the Medusozoa This work was supported by the National Science Foundation (Award Number 0002493) to M.J.Y. Programmatic support was provided by the Alfred P. Sloan Foundation. The authors acknowledge two anonymous reviewers who enhanced this manuscript. This study is a contribution from the Census of Marine Zooplankton (CMarZ, see www.CMarZ.org), a Census of Marine Life (CoML) field project Peer Reviewed 2013-06-25T06:49:01Z 2013-06-25T06:49:01Z 2010-12 2013-06-25T06:49:12Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.dsr2.2010.09.017 issn: 0967-0645 e-issn: 1879-0100 Deep-Sea Research Part II: Topical Studies in Oceanography 57(24-26): 2148-2156 (2010) http://hdl.handle.net/10261/78532 10.1016/j.dsr2.2010.09.017 en https://doi.org/10.1016/j.dsr2.2010.09.017 none Pergamon Press
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Medusozoa
DNA barcoding
mtCOI
Molecular identification
Gelatinous zooplankton
Medusozoa
DNA barcoding
mtCOI
Molecular identification
Gelatinous zooplankton
spellingShingle Medusozoa
DNA barcoding
mtCOI
Molecular identification
Gelatinous zooplankton
Medusozoa
DNA barcoding
mtCOI
Molecular identification
Gelatinous zooplankton
Ortman, Brian D.
Bucklin, Ann
Pagès, Francesc
Youngbluth, Marsh
DNA Barcoding the Medusozoa using mtCOI
description Special issue Species Diversity of Marine Zooplankton.-- 9 pages, 4 figures, 3 tables
format artículo
topic_facet Medusozoa
DNA barcoding
mtCOI
Molecular identification
Gelatinous zooplankton
author Ortman, Brian D.
Bucklin, Ann
Pagès, Francesc
Youngbluth, Marsh
author_facet Ortman, Brian D.
Bucklin, Ann
Pagès, Francesc
Youngbluth, Marsh
author_sort Ortman, Brian D.
title DNA Barcoding the Medusozoa using mtCOI
title_short DNA Barcoding the Medusozoa using mtCOI
title_full DNA Barcoding the Medusozoa using mtCOI
title_fullStr DNA Barcoding the Medusozoa using mtCOI
title_full_unstemmed DNA Barcoding the Medusozoa using mtCOI
title_sort dna barcoding the medusozoa using mtcoi
publisher Pergamon Press
publishDate 2010-12
url http://hdl.handle.net/10261/78532
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