Molecular and functional characterization of catfish (Heteropneustes fossilis) aquaporin-1b: Changes in expression during ovarian development and hormone-induced follicular maturation
10 pages, 6 figures
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2011-01
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| Subjects: | Aquaporin 1b, cDNA cloning and characterization, Catfishes, cAMP stimulation, Oocyte hydration, Vasotocin, Xenopus assay, |
| Online Access: | http://hdl.handle.net/10261/56939 |
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dig-icm-es-10261-569392020-11-26T09:50:59Z Molecular and functional characterization of catfish (Heteropneustes fossilis) aquaporin-1b: Changes in expression during ovarian development and hormone-induced follicular maturation Chaube, Radha Chauvigné, François Tingaud-Sequeira, Angèle Joy, Keerikkattil P. Acharjee, Arup Singh, Varsha Cerdà, Joan Aquaporin 1b cDNA cloning and characterization Catfishes cAMP stimulation Oocyte hydration Vasotocin Xenopus assay 10 pages, 6 figures The oocytes of the freshwater catfish Heteropneustes fossilis hydrate during hormone-induced meiotic maturation. To investigate if this process may be mediated by aquaporins (AQPs), as it occurs in marine fish producing highly hydrated eggs, the cloning of ovarian AQPs in catfish was carried out. Using degenerate primers for conserved domains of the major intrinsic protein (MIP) family, and 5′ and 3′end amplification procedures, a full-length cDNA encoding for an AQP1-like protein was isolated. The predicted protein showed the typical six transmembrane domains and two Asn-Pro-Ala (NPA) motifs conserved among the members of the AQP superfamily. Phylogenetic analysis indicated that the catfish AQP clustered with the teleost-specific aquaporin-1b subfamily, and accordingly it was termed HfAqp1b. Heterologous expression in Xenopus laevis oocytes indicated that HfAqp1b encoded for a functional AQP, water permeability being enhanced by cAMP. Site-directed mutagenesis revealed that cAMP induced the translocation of HfAqp1b into the oocyte plasma membrane most likely through the phosphorylation of HfAqp1b Ser227. In adult catfish, hfaqp1b transcripts were detected exclusively in ovary and brain and showed significant seasonal variations; in the ovary, hfaqp1b was maximally expressed during the pre-spawning period, whereas in the brain the highest expression was detected during spawning. In vitro stimulation of isolated catfish ovarian follicles with vasotocin (VT) or human chorionic gonadotropin (hCG), which induce oocyte maturation and hydration, elevated the hfaqp1b transcript levels after 6 or 16 h of incubation, respectively. These results suggest that HfAqp1b may play a role during VT- and hCG-induced oocyte hydration in catfish, and that VT may regulate HfAqp1b at the transcriptional and post-translational level in a manner similar to the vasopressin-dependent mammalian AQP2 This work was partially supported by grants from the DST and CSIR, New Delhi, to KPJ, and by a grant from the Spanish Ministry of Education and Science (AGL2007-60262-QUACYTE) to JC. FC was supported by a postdoctoral fellowship from Juan de la Cierva Programme (Spanish Ministry of Education and Science) Peer reviewed 2012-10-01T07:43:02Z 2012-10-01T07:43:02Z 2011-01 artículo http://purl.org/coar/resource_type/c_6501 General and Comparative Endocrinology 170(1): 162-171 (2011) 0016-6480 http://hdl.handle.net/10261/56939 10.1016/j.ygcen.2010.10.002 en https://doi.org/10.1016/j.ygcen.2010.10.002 none Elsevier |
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Aquaporin 1b cDNA cloning and characterization Catfishes cAMP stimulation Oocyte hydration Vasotocin Xenopus assay Aquaporin 1b cDNA cloning and characterization Catfishes cAMP stimulation Oocyte hydration Vasotocin Xenopus assay |
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Aquaporin 1b cDNA cloning and characterization Catfishes cAMP stimulation Oocyte hydration Vasotocin Xenopus assay Aquaporin 1b cDNA cloning and characterization Catfishes cAMP stimulation Oocyte hydration Vasotocin Xenopus assay Chaube, Radha Chauvigné, François Tingaud-Sequeira, Angèle Joy, Keerikkattil P. Acharjee, Arup Singh, Varsha Cerdà, Joan Molecular and functional characterization of catfish (Heteropneustes fossilis) aquaporin-1b: Changes in expression during ovarian development and hormone-induced follicular maturation |
| description |
10 pages, 6 figures |
| format |
artículo |
| topic_facet |
Aquaporin 1b cDNA cloning and characterization Catfishes cAMP stimulation Oocyte hydration Vasotocin Xenopus assay |
| author |
Chaube, Radha Chauvigné, François Tingaud-Sequeira, Angèle Joy, Keerikkattil P. Acharjee, Arup Singh, Varsha Cerdà, Joan |
| author_facet |
Chaube, Radha Chauvigné, François Tingaud-Sequeira, Angèle Joy, Keerikkattil P. Acharjee, Arup Singh, Varsha Cerdà, Joan |
| author_sort |
Chaube, Radha |
| title |
Molecular and functional characterization of catfish (Heteropneustes fossilis) aquaporin-1b: Changes in expression during ovarian development and hormone-induced follicular maturation |
| title_short |
Molecular and functional characterization of catfish (Heteropneustes fossilis) aquaporin-1b: Changes in expression during ovarian development and hormone-induced follicular maturation |
| title_full |
Molecular and functional characterization of catfish (Heteropneustes fossilis) aquaporin-1b: Changes in expression during ovarian development and hormone-induced follicular maturation |
| title_fullStr |
Molecular and functional characterization of catfish (Heteropneustes fossilis) aquaporin-1b: Changes in expression during ovarian development and hormone-induced follicular maturation |
| title_full_unstemmed |
Molecular and functional characterization of catfish (Heteropneustes fossilis) aquaporin-1b: Changes in expression during ovarian development and hormone-induced follicular maturation |
| title_sort |
molecular and functional characterization of catfish (heteropneustes fossilis) aquaporin-1b: changes in expression during ovarian development and hormone-induced follicular maturation |
| publisher |
Elsevier |
| publishDate |
2011-01 |
| url |
http://hdl.handle.net/10261/56939 |
| work_keys_str_mv |
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