Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis
8 pages, 6 figures.
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Elsevier
2011-02
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Subjects: | Embryo, Yolk metabolism, Gastrulation, Cathepsins, Enzyme processing, |
Online Access: | http://hdl.handle.net/10261/56338 |
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dig-icm-es-10261-563382020-11-26T06:47:53Z Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis Tingaud-Sequeira, Angèle Carnevali, Oliana Cerdà, Joan Embryo Yolk metabolism Gastrulation Cathepsins Enzyme processing 8 pages, 6 figures. The role of lysosomal proteases such as cathepsin B (Ctsb) and one of the paralogs of cathepsin L (Ctsla) during yolk metabolism in fish oocytes is well established. However, the function of Ctsb during embryogenesis, particularly in marine teleosts, has been poorly documented. In this study, the spatio-temporal expression of Ctsb and Ctsla, their enzymatic activities, and the processing of the Ctsb and its cellular localization, was investigated in developing embryos of the killifish (Fundulus heteroclitus). Both fhctsb and fhctsla transcript levels, as well as cathepsin B- and L-like activities, gradually increased in embryos from the 2–4 cell stage up to 7 days post-fertilization. During the morula to gastrula transition an increase of the active FhCtsb single chain form was followed by a rise in cathepsin B activity, which were apparently regulated by post-transcriptional mechanisms. During neurulation, a 8-fold increase in cathepsin B activity was accompanied by a more moderate increase in cathepsin L activity, which was 6-fold enhanced by 7 dpf. These increased catalytic activities were well-correlated to changes in the electrophoretic pattern of yolk proteins and a strong expression of fhctsb and its protein product in the yolk syncytial layer. The increase of cathepsin B activity was further correlated with an increment of the relative amount of the FhCtsb single and double chain forms, both active forms of FhCtsb. These results suggest that FhCtsb may be involved in the mechanisms underlying the onset of gastrulation in F. heteroclitus embryos, and may play complementary roles with FhCtsla during yolk metabolism Thisworkwasfinanced by grants fromthe SpanishMinistry of Science and Innovation (MICINN, AGL2004-00316 and HI2002-0051) to JC, and by a bilateral project Italy–Spain (Azioni Integrate Italia Spagna IT 1021 a.f. 2003) to OC. Peer reviewed 2012-09-14T10:55:03Z 2012-09-14T10:55:03Z 2011-02 artículo http://purl.org/coar/resource_type/c_6501 Comparative Biochemistry and Physiology - A - Molecular and Integrative Physiology 158(2): 221-228 (2011) 0010-406X http://hdl.handle.net/10261/56338 10.1016/j.cbpa.2010.11.002 en https://doi.org/10.1016/j.cbpa.2010.11.002 open Elsevier |
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Embryo Yolk metabolism Gastrulation Cathepsins Enzyme processing Embryo Yolk metabolism Gastrulation Cathepsins Enzyme processing |
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Embryo Yolk metabolism Gastrulation Cathepsins Enzyme processing Embryo Yolk metabolism Gastrulation Cathepsins Enzyme processing Tingaud-Sequeira, Angèle Carnevali, Oliana Cerdà, Joan Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis |
description |
8 pages, 6 figures. |
format |
artículo |
topic_facet |
Embryo Yolk metabolism Gastrulation Cathepsins Enzyme processing |
author |
Tingaud-Sequeira, Angèle Carnevali, Oliana Cerdà, Joan |
author_facet |
Tingaud-Sequeira, Angèle Carnevali, Oliana Cerdà, Joan |
author_sort |
Tingaud-Sequeira, Angèle |
title |
Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis |
title_short |
Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis |
title_full |
Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis |
title_fullStr |
Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis |
title_full_unstemmed |
Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis |
title_sort |
cathepsin b differential expression and enzyme processing and activity during fundulus heteroclitus embryogenesis |
publisher |
Elsevier |
publishDate |
2011-02 |
url |
http://hdl.handle.net/10261/56338 |
work_keys_str_mv |
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1777665863055310848 |