Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis

Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis

8 pages, 6 figures.

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Bibliographic Details
Main Authors: Tingaud-Sequeira, Angèle, Carnevali, Oliana, Cerdà, Joan
Format: artículo biblioteca
Language:English
Published: Elsevier 2011-02
Subjects:Embryo, Yolk metabolism, Gastrulation, Cathepsins, Enzyme processing,
Online Access:http://hdl.handle.net/10261/56338
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spelling dig-icm-es-10261-563382020-11-26T06:47:53Z Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis Tingaud-Sequeira, Angèle Carnevali, Oliana Cerdà, Joan Embryo Yolk metabolism Gastrulation Cathepsins Enzyme processing 8 pages, 6 figures. The role of lysosomal proteases such as cathepsin B (Ctsb) and one of the paralogs of cathepsin L (Ctsla) during yolk metabolism in fish oocytes is well established. However, the function of Ctsb during embryogenesis, particularly in marine teleosts, has been poorly documented. In this study, the spatio-temporal expression of Ctsb and Ctsla, their enzymatic activities, and the processing of the Ctsb and its cellular localization, was investigated in developing embryos of the killifish (Fundulus heteroclitus). Both fhctsb and fhctsla transcript levels, as well as cathepsin B- and L-like activities, gradually increased in embryos from the 2–4 cell stage up to 7 days post-fertilization. During the morula to gastrula transition an increase of the active FhCtsb single chain form was followed by a rise in cathepsin B activity, which were apparently regulated by post-transcriptional mechanisms. During neurulation, a 8-fold increase in cathepsin B activity was accompanied by a more moderate increase in cathepsin L activity, which was 6-fold enhanced by 7 dpf. These increased catalytic activities were well-correlated to changes in the electrophoretic pattern of yolk proteins and a strong expression of fhctsb and its protein product in the yolk syncytial layer. The increase of cathepsin B activity was further correlated with an increment of the relative amount of the FhCtsb single and double chain forms, both active forms of FhCtsb. These results suggest that FhCtsb may be involved in the mechanisms underlying the onset of gastrulation in F. heteroclitus embryos, and may play complementary roles with FhCtsla during yolk metabolism Thisworkwasfinanced by grants fromthe SpanishMinistry of Science and Innovation (MICINN, AGL2004-00316 and HI2002-0051) to JC, and by a bilateral project Italy–Spain (Azioni Integrate Italia Spagna IT 1021 a.f. 2003) to OC. Peer reviewed 2012-09-14T10:55:03Z 2012-09-14T10:55:03Z 2011-02 artículo http://purl.org/coar/resource_type/c_6501 Comparative Biochemistry and Physiology - A - Molecular and Integrative Physiology 158(2): 221-228 (2011) 0010-406X http://hdl.handle.net/10261/56338 10.1016/j.cbpa.2010.11.002 en https://doi.org/10.1016/j.cbpa.2010.11.002 open Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Embryo
Yolk metabolism
Gastrulation
Cathepsins
Enzyme processing
Embryo
Yolk metabolism
Gastrulation
Cathepsins
Enzyme processing
spellingShingle Embryo
Yolk metabolism
Gastrulation
Cathepsins
Enzyme processing
Embryo
Yolk metabolism
Gastrulation
Cathepsins
Enzyme processing
Tingaud-Sequeira, Angèle
Carnevali, Oliana
Cerdà, Joan
Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis
description 8 pages, 6 figures.
format artículo
topic_facet Embryo
Yolk metabolism
Gastrulation
Cathepsins
Enzyme processing
author Tingaud-Sequeira, Angèle
Carnevali, Oliana
Cerdà, Joan
author_facet Tingaud-Sequeira, Angèle
Carnevali, Oliana
Cerdà, Joan
author_sort Tingaud-Sequeira, Angèle
title Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis
title_short Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis
title_full Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis
title_fullStr Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis
title_full_unstemmed Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis
title_sort cathepsin b differential expression and enzyme processing and activity during fundulus heteroclitus embryogenesis
publisher Elsevier
publishDate 2011-02
url http://hdl.handle.net/10261/56338
work_keys_str_mv AT tingaudsequeiraangele cathepsinbdifferentialexpressionandenzymeprocessingandactivityduringfundulusheteroclitusembryogenesis
AT carnevalioliana cathepsinbdifferentialexpressionandenzymeprocessingandactivityduringfundulusheteroclitusembryogenesis
AT cerdajoan cathepsinbdifferentialexpressionandenzymeprocessingandactivityduringfundulusheteroclitusembryogenesis
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