miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells

miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells

12 pages, 13 figures, 2 tables, supplementary material https://doi.org/10.1016/j.ygeno.2024.110820.-- Data availability: Data will be made available on request

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Bibliographic Details
Main Authors: Gelderen, Tosca Agneta van, Ribas, Laia
Other Authors: Ministerio de Ciencia e Innovación (España)
Format: artículo biblioteca
Language:English
Published: Elsevier 2024-03
Subjects:Epigenetics, Cell culture, Reproduction, Infection, Gamete, Protocol, Marker, Conserve and sustainably use the oceans, seas and marine resources for sustainable development,
Online Access:http://hdl.handle.net/10261/356748
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100004837
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spelling dig-icm-es-10261-3567482024-05-13T10:31:26Z miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells Gelderen, Tosca Agneta van Ribas, Laia Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Epigenetics Cell culture Reproduction Infection Gamete Protocol Marker Conserve and sustainably use the oceans, seas and marine resources for sustainable development 12 pages, 13 figures, 2 tables, supplementary material https://doi.org/10.1016/j.ygeno.2024.110820.-- Data availability: Data will be made available on request microRNA-210 (miRNA), a well-documented miRNA, has been implicated in a myriad of biological processes, including responses to hypoxia, angiogenesis, cell proliferation, and male infertility in humans. However, a comprehensive understanding of its functions in fish requires further investigation. This study pursued to elucidate the downstream effect of dre-miR-210-5p on primary ovarian cell culture in zebrafish (Danio rerio), an animal model. A protocol was settled down by incubations with either an miR-210 mimic or a scrambled miRNA in the isolated ovaries. RNA-sequencing analysis identified ∼6000 differentially expressed target genes revealing that downregulated genes were associated with reproduction-related pathways while immune-related pathways displayed an upregulated pattern. To identify molecular markers, predicted target genes were classified into reproduction and immune cell types. These findings underscore the existence of a profound interplay between the reproductive and immune systems, with miR-210 emerging as a pivotal player in orchestrating transcriptomic alterations within fish ovaries This study was supported by the Spanish Ministry of Science and Innovation grant 2PID2020-113781RB-I00 “MicroMet” and by the Consejo Superior de Investigaciones Científicas (CSIC) grant 02030E004 “Interomics” to LR. [...] We thank the support by funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence accreditation (CEX2019–000928-S) to ICM Peer reviewed 2024-05-13T10:31:26Z 2024-05-13T10:31:26Z 2024-03 artículo http://purl.org/coar/resource_type/c_6501 Genomics 116(2): 110820 (2024) 0888-7543 CEX2019-000928-S http://hdl.handle.net/10261/356748 10.1016/j.ygeno.2024.110820 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004837 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113781RB-I00/ES/INTERACCIONES INMUNES Y REPRODUCTIVAS EN PECES: DESARROLLO DE MARCADORES DE MICROARNS, METILACION DEL ADN Y METABOLITOS (MICROMET)/ Publisher's version https://doi.org/10.1016/j.ygeno.2024.110820 Sí open Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Epigenetics
Cell culture
Reproduction
Infection
Gamete
Protocol
Marker
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Epigenetics
Cell culture
Reproduction
Infection
Gamete
Protocol
Marker
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
spellingShingle Epigenetics
Cell culture
Reproduction
Infection
Gamete
Protocol
Marker
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Epigenetics
Cell culture
Reproduction
Infection
Gamete
Protocol
Marker
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Gelderen, Tosca Agneta van
Ribas, Laia
miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells
description 12 pages, 13 figures, 2 tables, supplementary material https://doi.org/10.1016/j.ygeno.2024.110820.-- Data availability: Data will be made available on request
author2 Ministerio de Ciencia e Innovación (España)
author_facet Ministerio de Ciencia e Innovación (España)
Gelderen, Tosca Agneta van
Ribas, Laia
format artículo
topic_facet Epigenetics
Cell culture
Reproduction
Infection
Gamete
Protocol
Marker
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
author Gelderen, Tosca Agneta van
Ribas, Laia
author_sort Gelderen, Tosca Agneta van
title miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells
title_short miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells
title_full miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells
title_fullStr miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells
title_full_unstemmed miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells
title_sort mir-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells
publisher Elsevier
publishDate 2024-03
url http://hdl.handle.net/10261/356748
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100004837
work_keys_str_mv AT gelderentoscaagnetavan mir210promotesimmuneandsuppressesoocytemeiosisrelatedgenesinthezebrafishovariancells
AT ribaslaia mir210promotesimmuneandsuppressesoocytemeiosisrelatedgenesinthezebrafishovariancells
_version_ 1802820211111362560

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