Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide
14 pages, 8 figures, 3 tables, supplementary information https://doi.org/10.1038/s41598-018-22288-8
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2018-03
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dig-icm-es-10261-3467772024-02-12T20:10:25Z Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide Zhang, Qirui Kopp, Martina Babiak, Igor Fernandes, Jorge M. O. 14 pages, 8 figures, 3 tables, supplementary information https://doi.org/10.1038/s41598-018-22288-8 In many fish species, the immune system is significantly constrained by water temperature. In spite of its critical importance in protecting the host against pathogens, little is known about the influence of embryonic incubation temperature on the innate immunity of fish larvae. Zebrafish (Danio rerio) embryos were incubated at 24, 28 or 32 °C until first feeding. Larvae originating from each of these three temperature regimes were further distributed into three challenge temperatures and exposed to lipopolysaccharide (LPS) in a full factorial design (3 incubation × 3 challenge temperatures). At 24 h post LPS challenge, mortality of larvae incubated at 24 °C was 1.2 to 2.6-fold higher than those kept at 28 or 32 °C, regardless of the challenge temperature. LPS challenge at 24 °C stimulated similar immune-related processes but at different levels in larvae incubated at 24 or 32 °C, concomitantly with the down-regulation of some chemokine and lysozyme transcripts in the former group. Larvae incubated at 24 °C and LPS-challenged at 32 °C exhibited a limited immune response with up-regulation of hypoxia and oxidative stress processes. Annexin A2a, S100 calcium binding protein A10b and lymphocyte antigen-6, epidermis were identified as promising candidates for LPS recognition and signal transduction Peer reviewed 2024-02-12T12:49:54Z 2024-02-12T12:49:54Z 2018-03 artículo Scientific Reports 8: 4142 (2018) http://hdl.handle.net/10261/346777 10.1038/s41598-018-22288-8 2045-2322 29515182 en Publisher's version https://doi.org/10.1038/s41598-018-22288-8 No open Nature Publishing Group |
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14 pages, 8 figures, 3 tables, supplementary information https://doi.org/10.1038/s41598-018-22288-8 |
| format |
artículo |
| author |
Zhang, Qirui Kopp, Martina Babiak, Igor Fernandes, Jorge M. O. |
| spellingShingle |
Zhang, Qirui Kopp, Martina Babiak, Igor Fernandes, Jorge M. O. Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide |
| author_facet |
Zhang, Qirui Kopp, Martina Babiak, Igor Fernandes, Jorge M. O. |
| author_sort |
Zhang, Qirui |
| title |
Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide |
| title_short |
Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide |
| title_full |
Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide |
| title_fullStr |
Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide |
| title_full_unstemmed |
Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide |
| title_sort |
low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide |
| publisher |
Nature Publishing Group |
| publishDate |
2018-03 |
| url |
http://hdl.handle.net/10261/346777 |
| work_keys_str_mv |
AT zhangqirui lowincubationtemperatureduringearlydevelopmentnegativelyaffectssurvivalandrelatedinnateimmuneprocessesinzebrafishlarvaeexposedtolipopolysaccharide AT koppmartina lowincubationtemperatureduringearlydevelopmentnegativelyaffectssurvivalandrelatedinnateimmuneprocessesinzebrafishlarvaeexposedtolipopolysaccharide AT babiakigor lowincubationtemperatureduringearlydevelopmentnegativelyaffectssurvivalandrelatedinnateimmuneprocessesinzebrafishlarvaeexposedtolipopolysaccharide AT fernandesjorgemo lowincubationtemperatureduringearlydevelopmentnegativelyaffectssurvivalandrelatedinnateimmuneprocessesinzebrafishlarvaeexposedtolipopolysaccharide |
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1792484696356028416 |