High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry
pages, 7 figures.-- Data Availability Statement: Raw sequences are publicly available at the European Nucleotide Archive (https://www.ebi.ac.uk/ena) under the accession number PRJEB63614. The datasets generated for this study are available on request to the corresponding authors.
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| Format: | artículo biblioteca |
| Language: | English |
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Springer
2024
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| Subjects: | Osmotrophy, Mixotrophy, Eukaryotic communities, Diatoms, BONCAT, 61 HPG, Click-chemistry, Marine ecology, Single-cell microbiology, Conserve and sustainably use the oceans, seas and marine resources for sustainable development, |
| Online Access: | http://hdl.handle.net/10261/344807 |
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dig-icm-es-10261-3448072024-02-01T11:35:35Z High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry Mena Oliver, Catalina Deulofeu, Ona Forn, Irene Dordal Soriano, Júlia Mantilla-Arias, Yulieth A. Samos, Iván P. Sebastián, Marta Cardelús, Clara Massana, Ramon Romera-Castillo, Cristina Mallenco Fornies, Rebeca Gasol, Josep M. Ruiz-González, Clara Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) European Commission Osmotrophy Mixotrophy Eukaryotic communities Diatoms BONCAT, 61 HPG Click-chemistry Marine ecology Single-cell microbiology Conserve and sustainably use the oceans, seas and marine resources for sustainable development pages, 7 figures.-- Data Availability Statement: Raw sequences are publicly available at the European Nucleotide Archive (https://www.ebi.ac.uk/ena) under the accession number PRJEB63614. The datasets generated for this study are available on request to the corresponding authors. The osmotrophic uptake of dissolved organic compounds in the ocean is considered to be dominated by heterotrophic prokaryotes, whereas the role of planktonic eukaryotes is still unclear. We explored the capacity of natural eukaryotic plankton communities to incorporate the synthetic amino acid L-homopropargylglycine (HPG, analogue of methionine) using biorthogonal noncanonical amino acid tagging (BONCAT), and compared it with prokaryotic HPG use throughout a 9-day survey in the NW Mediterranean. BONCAT allows to fluorescently identify translationally active cells, but it has never been applied to natural eukaryotic communities. We found a large diversity of photosynthetic and heterotrophic eukaryotes incorporating HPG into proteins, with dinoflagellates and diatoms showing the highest percentages of BONCAT-labelled cells (49 ± 25% and 52 ± 15%, respectively). Among them, pennate diatoms exhibited higher HPG incorporation in the afternoon than in the morning, whereas small (≤5 μm) photosynthetic eukaryotes and heterotrophic nanoeukaryotes showed the opposite pattern. Centric diatoms (e.g., Chaetoceros, Thalassiosira, Lauderia spp.) dominated the eukaryotic HPG incorporation due to their high abundances and large sizes, accounting for up to 86% of the eukaryotic BONCAT signal, and strongly correlating with bulk 3H-leucine uptake rates. When including prokaryotes, eukaryotes were estimated to account for 19–31% of the bulk BONCAT signal. Our results evidence a large complexity in the osmotrophic uptake of HPG, which varies over time within and across eukaryotic groups, and highlights the potential of BONCAT to quantify osmotrophy and protein synthesis in complex eukaryotic communities This work was funded by the Spanish Ministry of Science, Innovation and Universities (MICINN) through the MIAU (RTI2018-101025-30-B-I00) and MICOLOR (PID2021-125469NB-C31) projects and the Ramon y Cajal contract to C.R.G. (RYC2019-026758-I), with funding from the Spanish Government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S). C.M. was supported by the Juan de la Cierva-formación fellowship (FJC2021-047745-34 I), funded by the Spanish Ministry of Science and Innovation (MCIN/AEI) and the EU (“NextGenerationEU”/PRTR) Peer reviewed 2024-02-01T11:35:35Z 2024-02-01T11:35:35Z 2024 artículo ISME Communications: ycae004 (2024) 2730-6151 CEX2019-000928-S http://hdl.handle.net/10261/344807 10.1093/ismeco/ycae004 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//RTI2018-101025-30-B-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125469NB-C31/ES/COLONIZACION MICROBIANA DE LAS PARTICULAS OCEANICAS: DE LA COMPRENSION MECANISTICA A LOS PATRONES GLOBALES : OCEANOGRAFIA MICROBIANA Y LA VIDA EN LAS PARTICULAS/ Preprint https://doi.org/10.1093/ismeco/ycae004 Sí open Springer |
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Osmotrophy Mixotrophy Eukaryotic communities Diatoms BONCAT, 61 HPG Click-chemistry Marine ecology Single-cell microbiology Conserve and sustainably use the oceans, seas and marine resources for sustainable development Osmotrophy Mixotrophy Eukaryotic communities Diatoms BONCAT, 61 HPG Click-chemistry Marine ecology Single-cell microbiology Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
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Osmotrophy Mixotrophy Eukaryotic communities Diatoms BONCAT, 61 HPG Click-chemistry Marine ecology Single-cell microbiology Conserve and sustainably use the oceans, seas and marine resources for sustainable development Osmotrophy Mixotrophy Eukaryotic communities Diatoms BONCAT, 61 HPG Click-chemistry Marine ecology Single-cell microbiology Conserve and sustainably use the oceans, seas and marine resources for sustainable development Mena Oliver, Catalina Deulofeu, Ona Forn, Irene Dordal Soriano, Júlia Mantilla-Arias, Yulieth A. Samos, Iván P. Sebastián, Marta Cardelús, Clara Massana, Ramon Romera-Castillo, Cristina Mallenco Fornies, Rebeca Gasol, Josep M. Ruiz-González, Clara High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry |
| description |
pages, 7 figures.-- Data Availability Statement: Raw sequences are publicly available at the European Nucleotide Archive (https://www.ebi.ac.uk/ena) under the accession number PRJEB63614. The datasets generated for this study are available on request to the corresponding authors. |
| author2 |
Ministerio de Ciencia, Innovación y Universidades (España) |
| author_facet |
Ministerio de Ciencia, Innovación y Universidades (España) Mena Oliver, Catalina Deulofeu, Ona Forn, Irene Dordal Soriano, Júlia Mantilla-Arias, Yulieth A. Samos, Iván P. Sebastián, Marta Cardelús, Clara Massana, Ramon Romera-Castillo, Cristina Mallenco Fornies, Rebeca Gasol, Josep M. Ruiz-González, Clara |
| format |
artículo |
| topic_facet |
Osmotrophy Mixotrophy Eukaryotic communities Diatoms BONCAT, 61 HPG Click-chemistry Marine ecology Single-cell microbiology Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
| author |
Mena Oliver, Catalina Deulofeu, Ona Forn, Irene Dordal Soriano, Júlia Mantilla-Arias, Yulieth A. Samos, Iván P. Sebastián, Marta Cardelús, Clara Massana, Ramon Romera-Castillo, Cristina Mallenco Fornies, Rebeca Gasol, Josep M. Ruiz-González, Clara |
| author_sort |
Mena Oliver, Catalina |
| title |
High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry |
| title_short |
High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry |
| title_full |
High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry |
| title_fullStr |
High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry |
| title_full_unstemmed |
High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry |
| title_sort |
high amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click-chemistry |
| publisher |
Springer |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10261/344807 |
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| _version_ |
1792484686715420672 |