Increased prokaryotic diversity in the Red Sea deep scattering layer
15 pages, 6 figures, supplementary information https://doi.org/10.1186/s40793-023-00542-5.-- Availability of data and materials: The raw sequences used in this study are available at https://www.ebi.ac.uk/ena/browser/view/PRJEB49545 as 67 paired fastq sequences with consecutive accession numbers: ERX7411972–ERX7412038. The metadata, processed sequence counts and taxonomic affiliation data that support the findings of this study are openly available in ZENODO at https://doi.org/10.5281/zenodo.5816123 (Huete-Stauffer et al. 2022). The code to replicate the main figures is available in the following repository https://github.com/tamaramegan/RedSea_DSL
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| Language: | English |
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BioMed Central
2023-12
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| Subjects: | Marine microbial ecology, Mesopelagic, Deep scattering layer, Diel vertical migration, Red Sea microbiome, rRNA diversity, Conserve and sustainably use the oceans, seas and marine resources for sustainable development, |
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dig-icm-es-10261-3439922024-01-26T12:43:00Z Increased prokaryotic diversity in the Red Sea deep scattering layer Huete-Stauffer, Tamara Logares, Ramiro Ansari, Mohd Ikram Røstad, Anders Calleja, Maria Ll. Morán, Xosé Anxelu G. King Abdullah University of Science and Technology Ministerio de Economía y Competitividad (España) Agencia Estatal de Investigación (España) Marine microbial ecology Mesopelagic Deep scattering layer Diel vertical migration Red Sea microbiome rRNA diversity Conserve and sustainably use the oceans, seas and marine resources for sustainable development 15 pages, 6 figures, supplementary information https://doi.org/10.1186/s40793-023-00542-5.-- Availability of data and materials: The raw sequences used in this study are available at https://www.ebi.ac.uk/ena/browser/view/PRJEB49545 as 67 paired fastq sequences with consecutive accession numbers: ERX7411972–ERX7412038. The metadata, processed sequence counts and taxonomic affiliation data that support the findings of this study are openly available in ZENODO at https://doi.org/10.5281/zenodo.5816123 (Huete-Stauffer et al. 2022). The code to replicate the main figures is available in the following repository https://github.com/tamaramegan/RedSea_DSL Background: The diel vertical migration (DVM) of fish provides an active transport of labile dissolved organic matter (DOM) to the deep ocean, fueling the metabolism of heterotrophic bacteria and archaea. We studied the impact of DVM on the mesopelagic prokaryotic diversity of the Red Sea focusing on the mesopelagic deep scattering layer (DSL) between 450–600 m. Results: Despite the general consensus of homogeneous conditions in the mesopelagic layer, we observed variability in physico-chemical variables (oxygen, inorganic nutrients, DOC) in the depth profiles. We also identified distinct seasonal indicator prokaryotes inhabiting the DSL, representing between 2% (in spring) to over 10% (in winter) of total 16S rRNA gene sequences. The dominant indicator groups were Alteromonadales in winter, Vibrionales in spring and Microtrichales in summer. Using multidimensional scaling analysis, the DSL samples showed divergence from the surrounding mesopelagic layers and were distributed according to depth (47% of variance explained). We identified the sources of diversity that contribute to the DSL by analyzing the detailed profiles of spring, where 3 depths were sampled in the mesopelagic. On average, 7% was related to the epipelagic, 34% was common among the other mesopelagic waters and 38% was attributable to the DSL, with 21% of species being unique to this layer. Conclusions: We conclude that the mesopelagic physico-chemical properties shape a rather uniform prokaryotic community, but that the 200 m deep DSL contributes uniquely and in a high proportion to the diversity of the Red Sea mesopelagic This work was funded through the Center Competitive Fund (2016–2018) from King Abdullah University of Science and Technology. RL was supported by a Ramón y Cajal fellowship (RYC-2013-12554, MINECO, Spain With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) Peer reviewed 2024-01-26T12:42:59Z 2024-01-26T12:42:59Z 2023-12 artículo Environmental Microbiome 18: 87 (2023) 2524-6372 CEX2019-000928-S http://hdl.handle.net/10261/343992 10.1186/s40793-023-00542-5 en Publisher's version https://doi.org/10.1186/s40793-023-00542-5 Sí open BioMed Central |
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Marine microbial ecology Mesopelagic Deep scattering layer Diel vertical migration Red Sea microbiome rRNA diversity Conserve and sustainably use the oceans, seas and marine resources for sustainable development Marine microbial ecology Mesopelagic Deep scattering layer Diel vertical migration Red Sea microbiome rRNA diversity Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
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Marine microbial ecology Mesopelagic Deep scattering layer Diel vertical migration Red Sea microbiome rRNA diversity Conserve and sustainably use the oceans, seas and marine resources for sustainable development Marine microbial ecology Mesopelagic Deep scattering layer Diel vertical migration Red Sea microbiome rRNA diversity Conserve and sustainably use the oceans, seas and marine resources for sustainable development Huete-Stauffer, Tamara Logares, Ramiro Ansari, Mohd Ikram Røstad, Anders Calleja, Maria Ll. Morán, Xosé Anxelu G. Increased prokaryotic diversity in the Red Sea deep scattering layer |
| description |
15 pages, 6 figures, supplementary information https://doi.org/10.1186/s40793-023-00542-5.-- Availability of data and materials: The raw sequences used in this study are available at https://www.ebi.ac.uk/ena/browser/view/PRJEB49545 as 67 paired fastq sequences with consecutive accession numbers: ERX7411972–ERX7412038. The metadata, processed sequence counts and taxonomic affiliation data that support the findings of this study are openly available in ZENODO at https://doi.org/10.5281/zenodo.5816123 (Huete-Stauffer et al. 2022). The code to replicate the main figures is available in the following repository https://github.com/tamaramegan/RedSea_DSL |
| author2 |
King Abdullah University of Science and Technology |
| author_facet |
King Abdullah University of Science and Technology Huete-Stauffer, Tamara Logares, Ramiro Ansari, Mohd Ikram Røstad, Anders Calleja, Maria Ll. Morán, Xosé Anxelu G. |
| format |
artículo |
| topic_facet |
Marine microbial ecology Mesopelagic Deep scattering layer Diel vertical migration Red Sea microbiome rRNA diversity Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
| author |
Huete-Stauffer, Tamara Logares, Ramiro Ansari, Mohd Ikram Røstad, Anders Calleja, Maria Ll. Morán, Xosé Anxelu G. |
| author_sort |
Huete-Stauffer, Tamara |
| title |
Increased prokaryotic diversity in the Red Sea deep scattering layer |
| title_short |
Increased prokaryotic diversity in the Red Sea deep scattering layer |
| title_full |
Increased prokaryotic diversity in the Red Sea deep scattering layer |
| title_fullStr |
Increased prokaryotic diversity in the Red Sea deep scattering layer |
| title_full_unstemmed |
Increased prokaryotic diversity in the Red Sea deep scattering layer |
| title_sort |
increased prokaryotic diversity in the red sea deep scattering layer |
| publisher |
BioMed Central |
| publishDate |
2023-12 |
| url |
http://hdl.handle.net/10261/343992 |
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