Molecular characterization of Calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo
10 pages, 6 figures
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| Main Authors: | , , , |
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| Format: | artículo biblioteca |
| Language: | English |
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American Association for Anatomy
2002-06
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| Subjects: | Zebrafish embryogenesis, Notochord, Sheath, Extracellular matrix, No tail mutant, |
| Online Access: | http://hdl.handle.net/10261/342629 |
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dig-icm-es-10261-3426292024-01-24T09:26:46Z Molecular characterization of Calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael Zebrafish embryogenesis Notochord Sheath Extracellular matrix No tail mutant 10 pages, 6 figures During the screening of a zebrafish postsomitogenesis embryo cDNA library, we have identified a cDNA corresponding to a novel type of protein localized to the notochordal sheath-associated extracellular matrix (ECM) of the embryo. The 4.049-kb mRNA encodes a predicted polypeptide of 1,207 amino acids (122 kDa, pI 10.50) with a potential signal peptide of 20 amino acids. After the signal peptide, the mature protein consists of 1,187 amino acids (119 kDa, pI 10.46), for which the name “Calymmin” (from Greek χαλνμμα, to envelop, to cover) is proposed. The Calymmin mRNA is highly and transiently expressed by the notochord cells of the embryo from the 10- to 12-somite stage to the pharyngula period (13 and 24 hours postfertilization, respectively), and light and electron microscopical immunolocalization analysis revealed that the protein was specifically localized within a granular and filamentous layer of the ECM compartment surrounding the notochord. In zebrafish no tail mutants (ntltc41), in which the notochord precursor cells are present but fail to differentiate, the Calymmin protein was not detected, confirming the notochord origin of Calymmin. These results indicate that Calymmin is a novel constitutive protein of the ECM compartment associated to the perinotochordal sheath in the zebrafish embryo, which is specifically expressed by the differentiating notochord cells. © 2002 Wiley-Liss, Inc. Peer reviewed 2024-01-17T11:24:27Z 2024-01-17T11:24:27Z 2002-06 artículo Developmental Dynamics 224(2): 200-209 (2002) 1058-8388 http://hdl.handle.net/10261/342629 10.1002/dvdy.10101 1097-0177 en https://doi.org/10.1002/dvdy.10101 No open American Association for Anatomy |
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English |
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Zebrafish embryogenesis Notochord Sheath Extracellular matrix No tail mutant Zebrafish embryogenesis Notochord Sheath Extracellular matrix No tail mutant |
| spellingShingle |
Zebrafish embryogenesis Notochord Sheath Extracellular matrix No tail mutant Zebrafish embryogenesis Notochord Sheath Extracellular matrix No tail mutant Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael Molecular characterization of Calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo |
| description |
10 pages, 6 figures |
| format |
artículo |
| topic_facet |
Zebrafish embryogenesis Notochord Sheath Extracellular matrix No tail mutant |
| author |
Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael |
| author_facet |
Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael |
| author_sort |
Cerdà, Joan |
| title |
Molecular characterization of Calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo |
| title_short |
Molecular characterization of Calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo |
| title_full |
Molecular characterization of Calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo |
| title_fullStr |
Molecular characterization of Calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo |
| title_full_unstemmed |
Molecular characterization of Calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo |
| title_sort |
molecular characterization of calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo |
| publisher |
American Association for Anatomy |
| publishDate |
2002-06 |
| url |
http://hdl.handle.net/10261/342629 |
| work_keys_str_mv |
AT cerdajoan molecularcharacterizationofcalymminanovelnotochordsheathassociatedextracellularmatrixproteininthezebrafishembryo AT grundchristine molecularcharacterizationofcalymminanovelnotochordsheathassociatedextracellularmatrixproteininthezebrafishembryo AT frankewernerw molecularcharacterizationofcalymminanovelnotochordsheathassociatedextracellularmatrixproteininthezebrafishembryo AT brandmichael molecularcharacterizationofcalymminanovelnotochordsheathassociatedextracellularmatrixproteininthezebrafishembryo |
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1792484672333152256 |