Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation
9 pages, 4 figures, 4 tables, supplementary data https://doi.org/10.1016/j.chemosphere.2023.140770.-- Data will be made available on request
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| Language: | English |
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Elsevier
2024-01
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| Subjects: | Cholinesterases, Carboxylesterases, Sea turtles, Caretta caretta, Marine litter, Plastic additives, Conserve and sustainably use the oceans, seas and marine resources for sustainable development, |
| Online Access: | http://hdl.handle.net/10261/340953 |
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dig-icm-es-10261-3409532023-12-19T12:45:47Z Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation Omedes Martínez, Sergi Crespo-Picazo, José Luis Robinson, Nathan J. García-Parraga, Daniel Solé, Montserrat Agencia Estatal de Investigación (España) Consejo Superior de Investigaciones Científicas (España) Cholinesterases Carboxylesterases Sea turtles Caretta caretta Marine litter Plastic additives Conserve and sustainably use the oceans, seas and marine resources for sustainable development 9 pages, 4 figures, 4 tables, supplementary data https://doi.org/10.1016/j.chemosphere.2023.140770.-- Data will be made available on request Sea turtles are frequently proposed as indicator species for assessing ocean health. To faciliate the use of these species as bioindicators requires the development of tools for rapidly and effectively assessing individual health. Here, we collected 104 blood samples from 69 loggerhead sea turtles, Caretta caretta, undergoing rehabilitation to determine the connection between health status, the activity of B-esterases, and other biochemical parameters. To determine the optimal assay protocol for B-esterases, we measured the activity and kinetics of cholinesterases-(ChEs) and carboxylesterases (CEs) using 3 and 5 commercial substrates, respectively, at different assay conditions. IC50 values for the activity of B-esterases were calculated within a concentration range for model pesticide inhibitors. Turtles’ health status was determined via routine veterinary procedures. During rehabilitation (which was associated with improving health status), we observed a decrease in the activity of most enzymes (especially in acetylcholinesterase) alongside an increase in CE when using p-nitrophenyl acetate as a substrate. As such, it is possible that the activity rates of plasmatic B-esterases could serve as an indicator of health status. There is also high potential that B-esterases could be specifically sensitive to marine pollutants although to further validate this would require future studies to specifically correlate B-esterarse activities to pollutant concentrations in blood or excreta To the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX 2019-000928-S). This work has received funding from CSIC Interdisciplinary Thematic Platform (PTI+) Interdisciplinary Platform for Sustainable Plastics towards a Circular Economy+ (PTI-SusPlast+) Peer reviewed 2023-12-19T12:45:46Z 2023-12-19T12:45:46Z 2024-01 artículo Chemosphere 348: 140770 (2024) 0045-6535 CEX2019-000928-S http://hdl.handle.net/10261/340953 10.1016/j.chemosphere.2023.140770 en Publisher's version https://doi.org/10.1016/j.chemosphere.2023.140770 Sí open Elsevier |
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Cholinesterases Carboxylesterases Sea turtles Caretta caretta Marine litter Plastic additives Conserve and sustainably use the oceans, seas and marine resources for sustainable development Cholinesterases Carboxylesterases Sea turtles Caretta caretta Marine litter Plastic additives Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
| spellingShingle |
Cholinesterases Carboxylesterases Sea turtles Caretta caretta Marine litter Plastic additives Conserve and sustainably use the oceans, seas and marine resources for sustainable development Cholinesterases Carboxylesterases Sea turtles Caretta caretta Marine litter Plastic additives Conserve and sustainably use the oceans, seas and marine resources for sustainable development Omedes Martínez, Sergi Crespo-Picazo, José Luis Robinson, Nathan J. García-Parraga, Daniel Solé, Montserrat Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation |
| description |
9 pages, 4 figures, 4 tables, supplementary data https://doi.org/10.1016/j.chemosphere.2023.140770.-- Data will be made available on request |
| author2 |
Agencia Estatal de Investigación (España) |
| author_facet |
Agencia Estatal de Investigación (España) Omedes Martínez, Sergi Crespo-Picazo, José Luis Robinson, Nathan J. García-Parraga, Daniel Solé, Montserrat |
| format |
artículo |
| topic_facet |
Cholinesterases Carboxylesterases Sea turtles Caretta caretta Marine litter Plastic additives Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
| author |
Omedes Martínez, Sergi Crespo-Picazo, José Luis Robinson, Nathan J. García-Parraga, Daniel Solé, Montserrat |
| author_sort |
Omedes Martínez, Sergi |
| title |
Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation |
| title_short |
Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation |
| title_full |
Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation |
| title_fullStr |
Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation |
| title_full_unstemmed |
Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation |
| title_sort |
identifying biomarkers of pollutant exposure in ocean sentinels: characterisation and optimisation of b-esterases in plasma from loggerhead turtles undergoing rehabilitation |
| publisher |
Elsevier |
| publishDate |
2024-01 |
| url |
http://hdl.handle.net/10261/340953 |
| work_keys_str_mv |
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| _version_ |
1787227011124559872 |