Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms

Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms

10 pages, 5 figures, 1 table, supplementary information https://doi.org/10.1039/D2AY02019J

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Main Authors: Medina-Pérez, Noemí Inmaculada, Santos, Francisco Javier, Berdalet, Elisa, Moyano, Encarnación
Other Authors: Generalitat de Catalunya
Format: artículo biblioteca
Language:English
Published: Royal Society of Chemistry (UK) 2023-03
Subjects:Conserve and sustainably use the oceans, seas and marine resources for sustainable development,
Online Access:http://hdl.handle.net/10261/330512
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spelling dig-icm-es-10261-3305122023-07-05T10:05:53Z Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms Medina-Pérez, Noemí Inmaculada Santos, Francisco Javier Berdalet, Elisa Moyano, Encarnación Generalitat de Catalunya Ministerio de Ciencia, Innovación y Universidades (España) European Commission European Research Council Agencia Estatal de Investigación (España) Conserve and sustainably use the oceans, seas and marine resources for sustainable development 10 pages, 5 figures, 1 table, supplementary information https://doi.org/10.1039/D2AY02019J Analogues of palytoxin (PLTX), one of the most potent marine biotoxins, are produced by some species of the marine dinoflagellates of the genus Ostreopsis. The proliferation of these species in different coastal zones represents a potential threat of seafood poisoning in humans because the produced toxins can be transferred through marine food webs. Thus, the determination of the concentration of PLTX analogues (ovatoxins—OVTXs, ostreocins—OSTs and isobaric PLTX) in different matrices (seawater, marine fauna, etc.) is necessary to protect human health. This study is addressed to overcome some of the challenges that the chemical complexity of these molecules poses to their quantification by ultra-high-performance liquid chromatography high-resolution mass spectrometry-based techniques (UHPLC-HRMS). In particular, the mass spectra of the palytoxin analogues show the presence of a large number of ions (including mono- and multiply charged ions) whose nature, relative abundances and behavior can lead to quantitation errors if the correct ions are not selected. In this work, the variability of the PLTX and OVTX profiles under different instrument conditions, including the use of diverse electrospray generation sources and different quantitation methods, is studied. Moreover, the extraction protocol in seawater containing Ostreopsis sp. ovata cells is also evaluated. The use of a heated electrospray operating at 350 °C and a quantitative method including ions from different multiply charged species provides a more robust and reliable method for overcoming the problems due to the variability in the toxin's mass spectrum profile. A single MeOH : H2O (80 : 20, v/v) extraction is proposed as the best and reliable procedure. The overall method proposed was applied to quantify OVTXs (-a to -g) and iso-PLTX along the 2019 Ostreopsis cf. ovata bloom. The cells contained a total toxin concentration of up to 20.39 pg per cell The authors thank the different financing agencies that supported the study. N. I. M.-P. was granted the fellowship FISDUR2020-00254 (AGAUR, Government of Catalonia). Financial support: MICIU (Spain, project PGC2018-095013-B-I00), AGAUR (Generalitat de Catalunya, project 2017SGR-310) and the Water Research Institute (IdRA, University of Barcelona). Support was provided also by the CoCliME project, an ERA4CS network (ERA-NET) initiated by JPI Climate and co-financed by FORMAS (SE) and the European Union (Grant no. 690462), ERC-2019-CoG-863934-GlycoSpec (European Research Council Consolidator Grant) With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) Peer reviewed 2023-07-05T10:04:08Z 2023-07-05T10:04:08Z 2023-03 artículo Analytical Methods 10: 1355-1364 (2023) 1759-9660 CEX2019-000928-S http://hdl.handle.net/10261/330512 10.1039/D2AY02019J 1759-9679 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-095013-B-I00/ES/METODOLOGIAS RECIENTES EN ESPECTROMETRIA DE MASAS PARA ANALISIS DE CONTROL Y ALTO RENDIMIENTO/ info:eu-repo/grantAgreement/EC/H2020/690462 Postprint https://doi.org/10.1039/D2AY02019J Sí embargo_20240331 Royal Society of Chemistry (UK)
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
spellingShingle Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Medina-Pérez, Noemí Inmaculada
Santos, Francisco Javier
Berdalet, Elisa
Moyano, Encarnación
Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
description 10 pages, 5 figures, 1 table, supplementary information https://doi.org/10.1039/D2AY02019J
author2 Generalitat de Catalunya
author_facet Generalitat de Catalunya
Medina-Pérez, Noemí Inmaculada
Santos, Francisco Javier
Berdalet, Elisa
Moyano, Encarnación
format artículo
topic_facet Conserve and sustainably use the oceans, seas and marine resources for sustainable development
author Medina-Pérez, Noemí Inmaculada
Santos, Francisco Javier
Berdalet, Elisa
Moyano, Encarnación
author_sort Medina-Pérez, Noemí Inmaculada
title Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_short Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_full Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_fullStr Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_full_unstemmed Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_sort multiply charged ion profiles in the uhplc-hrms analysis of palytoxin analogues from ostreopsis cf. ovata blooms
publisher Royal Society of Chemistry (UK)
publishDate 2023-03
url http://hdl.handle.net/10261/330512
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AT santosfranciscojavier multiplychargedionprofilesintheuhplchrmsanalysisofpalytoxinanaloguesfromostreopsiscfovatablooms
AT berdaletelisa multiplychargedionprofilesintheuhplchrmsanalysisofpalytoxinanaloguesfromostreopsiscfovatablooms
AT moyanoencarnacion multiplychargedionprofilesintheuhplchrmsanalysisofpalytoxinanaloguesfromostreopsiscfovatablooms
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