Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms
17 pages, 5 figures, supplementary information https://doi.org/10.1038/s41467-023-36049-3.-- Data availability: All data needed to evaluate the conclusions in the paper are present in the paper and clearly indicated in the Methods. Flow cytometry, nutrient, and temperature data are available in Dryad: https://doi.org/10.5061/dryad.q573n5tfr. Flowcam data is available on Ecotaxa under the project “Flowcam Composite Aquacosm_2018_VIMS-Ehux” (https://ecotaxa.obs-vlfr.fr/prj/2501). Sequencing data has been deposited under NCBI Bioproject PRJNA694552: 16S data is available under Biosample SAMN17576248 and 18S data is available under Biosample SAMN20295136. The PR2 database can be found on Zenodo: zenodo.org/record/5031733. Assembled sequences deposited on NCBI with accession numbers MZ562737, MZ562738, MZ562739, MZ562740, MZ562741. All data used to produce figures are available in the Source Data file. Source data are provided with this paper.-- Code availability: The code for all the analyses is available on Mendeley at https://doi.org/10.17632/k33xvnxhdb.1 (https://data.mendeley.com/datasets/k33xvnxhdb/1)
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Conserve and sustainably use the oceans, seas and marine resources for sustainable development Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
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Conserve and sustainably use the oceans, seas and marine resources for sustainable development Conserve and sustainably use the oceans, seas and marine resources for sustainable development Vincent, Flora Gralka, Matti Schleyer, Guy Schatz, Daniella Cabrera-Brufau, Miguel Kuhlisch, Constanze Sichert, Andreas Vidal-Melgosa, Silvia Mayers, Kyle Barak-Gavish, Noa Flores, J. Michel Masdeu Navarro, Marta Egge, Jorun Karin Larsen, Aud Hehemann, Jan-Hendrik Marrasé, Cèlia Simó, Rafel Cordero, Otto X. Vardi, Assaf Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms |
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17 pages, 5 figures, supplementary information https://doi.org/10.1038/s41467-023-36049-3.-- Data availability: All data needed to evaluate the conclusions in the paper are present in the paper and clearly indicated in the Methods. Flow cytometry, nutrient, and temperature data are available in Dryad: https://doi.org/10.5061/dryad.q573n5tfr. Flowcam data is available on Ecotaxa under the project “Flowcam Composite Aquacosm_2018_VIMS-Ehux” (https://ecotaxa.obs-vlfr.fr/prj/2501). Sequencing data has been deposited under NCBI Bioproject PRJNA694552: 16S data is available under Biosample SAMN17576248 and 18S data is available under Biosample SAMN20295136. The PR2 database can be found on Zenodo: zenodo.org/record/5031733. Assembled sequences deposited on NCBI with accession numbers MZ562737, MZ562738, MZ562739, MZ562740, MZ562741. All data used to produce figures are available in the Source Data file. Source data are provided with this paper.-- Code availability: The code for all the analyses is available on Mendeley at https://doi.org/10.17632/k33xvnxhdb.1 (https://data.mendeley.com/datasets/k33xvnxhdb/1) |
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European Research Council Vincent, Flora Gralka, Matti Schleyer, Guy Schatz, Daniella Cabrera-Brufau, Miguel Kuhlisch, Constanze Sichert, Andreas Vidal-Melgosa, Silvia Mayers, Kyle Barak-Gavish, Noa Flores, J. Michel Masdeu Navarro, Marta Egge, Jorun Karin Larsen, Aud Hehemann, Jan-Hendrik Marrasé, Cèlia Simó, Rafel Cordero, Otto X. Vardi, Assaf |
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Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
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Vincent, Flora Gralka, Matti Schleyer, Guy Schatz, Daniella Cabrera-Brufau, Miguel Kuhlisch, Constanze Sichert, Andreas Vidal-Melgosa, Silvia Mayers, Kyle Barak-Gavish, Noa Flores, J. Michel Masdeu Navarro, Marta Egge, Jorun Karin Larsen, Aud Hehemann, Jan-Hendrik Marrasé, Cèlia Simó, Rafel Cordero, Otto X. Vardi, Assaf |
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Vincent, Flora |
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Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms |
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Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms |
| title_full |
Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms |
| title_fullStr |
Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms |
| title_full_unstemmed |
Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms |
| title_sort |
viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms |
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Nature Publishing Group |
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2023-01 |
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http://hdl.handle.net/10261/305420 |
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dig-icm-es-10261-3054202023-04-03T10:45:04Z Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms Vincent, Flora Gralka, Matti Schleyer, Guy Schatz, Daniella Cabrera-Brufau, Miguel Kuhlisch, Constanze Sichert, Andreas Vidal-Melgosa, Silvia Mayers, Kyle Barak-Gavish, Noa Flores, J. Michel Masdeu Navarro, Marta Egge, Jorun Karin Larsen, Aud Hehemann, Jan-Hendrik Marrasé, Cèlia Simó, Rafel Cordero, Otto X. Vardi, Assaf European Research Council Simons Foundation Weizmann Institute of Science Israel Academy of Sciences and Humanities European Commission Max Planck Society Deutsche Forschungsgemeinschaft Ministerio de Economía y Competitividad (España) Agencia Estatal de Investigación (España) Conserve and sustainably use the oceans, seas and marine resources for sustainable development 17 pages, 5 figures, supplementary information https://doi.org/10.1038/s41467-023-36049-3.-- Data availability: All data needed to evaluate the conclusions in the paper are present in the paper and clearly indicated in the Methods. Flow cytometry, nutrient, and temperature data are available in Dryad: https://doi.org/10.5061/dryad.q573n5tfr. Flowcam data is available on Ecotaxa under the project “Flowcam Composite Aquacosm_2018_VIMS-Ehux” (https://ecotaxa.obs-vlfr.fr/prj/2501). Sequencing data has been deposited under NCBI Bioproject PRJNA694552: 16S data is available under Biosample SAMN17576248 and 18S data is available under Biosample SAMN20295136. The PR2 database can be found on Zenodo: zenodo.org/record/5031733. Assembled sequences deposited on NCBI with accession numbers MZ562737, MZ562738, MZ562739, MZ562740, MZ562741. All data used to produce figures are available in the Source Data file. Source data are provided with this paper.-- Code availability: The code for all the analyses is available on Mendeley at https://doi.org/10.17632/k33xvnxhdb.1 (https://data.mendeley.com/datasets/k33xvnxhdb/1) Algal blooms are hotspots of marine primary production and play central roles in microbial ecology and global elemental cycling. Upon demise of the bloom, organic carbon is partly respired and partly transferred to either higher trophic levels, bacterial biomass production or sinking. Viral infection can lead to bloom termination, but its impact on the fate of carbon remains largely unquantified. Here, we characterize the interplay between viral infection and the composition of a bloom-associated microbiome and consequently the evolving biogeochemical landscape, by conducting a large-scale mesocosm experiment where we monitor seven induced coccolithophore blooms. The blooms show different degrees of viral infection and reveal that only high levels of viral infection are followed by significant shifts in the composition of free-living bacterial and eukaryotic assemblages. Intriguingly, upon viral infection the biomass of eukaryotic heterotrophs (thraustochytrids) rivals that of bacteria as potential recyclers of organic matter. By combining modeling and quantification of active viral infection at a single-cell resolution, we estimate that viral infection causes a 2–4 fold increase in per-cell rates of extracellular carbon release in the form of acidic polysaccharides and particulate inorganic carbon, two major contributors to carbon sinking into the deep ocean. These results reveal the impact of viral infection on the fate of carbon through microbial recyclers of organic matter in large-scale coccolithophore blooms This research was supported by the European Research Council CoG (VIROCELLSPHERE grant no. 681715, A.V.), the European Research Council AdV (VIBES, grant no. 101053543, A.V.) and the Simons foundation grant (no. 735079, A.V.) “Untangling the infection outcome of host-virus dynamics in algal blooms in the ocean” and the Dean of Faculty Fellowship of the Weizmann Institute of Science and Israeli Academy of Science and Humanities awarded to F.V. The mesocosm experiment VIMS-Ehux was supported by EU Horizon2020-INFRAIA project AQUACOSM (grant no. 731065, A.V.). The NA-VICE cruise was supported by the NSF (grant no. OCE-1059884). J.-H.H. was supported by the Max Planck Society and by the Deutsche Forschungsgemeinschaft (DFG) Emmy Noether grant HE 7217/1-1, and through the Cluster of Excellence “The Ocean Floor—Earth’s Uncharted Interface” project 390741603. O.X.C. was supported by the Simons Collaboration: Principles of Microbial Ecosystems (PriME) award no. 542395. M.G. was supported by Simons Foundation Postdoctoral Fellowship Award 599207. The ICM-CSIC group acknowledges funding from Spanish Ministry of Science and Innovation (MCIN/AEI, doi: 10.13039/501100011033) through the BIOGAPS grant (CTM2016–81008–R) and the “Severo Ochoa Centre of Excellence” accreditation (CEX2019-000298-S), and from the European Research Council under the EU’s Horizon 2020 research and innovation programme through the SUMMIT grant (ERC-2018-AdG#834162) Peer reviewed 2023-04-03T10:45:04Z 2023-04-03T10:45:04Z 2023-01 artículo Nature Communications 14: 510 (2023) CEX2019-000928-S http://hdl.handle.net/10261/305420 10.1038/s41467-023-36049-3 2041-1723 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/731065 info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2016-81008-R Publisher's version https://doi.org/10.1038/s41467-023-36049-3 Sí open Nature Publishing Group |