Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities
31 pages, 6 figures, supplemental Information https://doi.org/10.7717/peerj.13642.-- DNA Deposition: The following information was supplied regarding the deposition of DNA sequences: The sequence data are available at the European Nucleotide Archive (ENA) database: PRJEB41739 .-- Data Availability: The following information was supplied regarding data availability: The data is available at Figshare: https://figshare.com/projects/Hidden_interactions_in_the_intertidal_rocky_shore_variation_in_pedal_mucus_microbiomes_among_marine_grazers_that_feed_on_epilithic_biofilm_communities_/119655 Arboleda Baena, Clara María (2021): PedalMucusEffect_PhotosyntheticBiofilmCoverPercentage. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170586.v1 Arboleda Baena, Clara María (2021): PedalMucus_MANOVA_Carbohydrates/ProteinsContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170523.v1 Arboleda Baena, Clara María (2021): PedalMucus_ProteinsContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170520.v1 Arboleda Baena, Clara María (2021): PedalMucus_CarbohydratesContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170514.v1. Arboleda Baena, Clara María (2021): GrazerAssemblageBiomass.csv. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15113490.v2. Arboleda Baena, Clara María (2021): PedalMucus_pH. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15115059.v2
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2022-09
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| Subjects: | Mollusk, Grazers, Epilithic biofilm, Intertidal, Pedal mucus microbiota, |
| Online Access: | http://hdl.handle.net/10261/284479 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100002848 http://dx.doi.org/10.13039/501100009610 |
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Mollusk Grazers Epilithic biofilm Intertidal Pedal mucus microbiota Mollusk Grazers Epilithic biofilm Intertidal Pedal mucus microbiota |
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Mollusk Grazers Epilithic biofilm Intertidal Pedal mucus microbiota Mollusk Grazers Epilithic biofilm Intertidal Pedal mucus microbiota Arboleda-Baena, Clara Pareja, Claudia Belén Pla, Isidora Logares, Ramiro De la Iglesia, Rodrigo Navarrete, Sergio A. Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities |
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31 pages, 6 figures, supplemental Information https://doi.org/10.7717/peerj.13642.-- DNA Deposition: The following information was supplied regarding the deposition of DNA sequences:
The sequence data are available at the European Nucleotide Archive (ENA) database: PRJEB41739
.-- Data Availability: The following information was supplied regarding data availability:
The data is available at Figshare: https://figshare.com/projects/Hidden_interactions_in_the_intertidal_rocky_shore_variation_in_pedal_mucus_microbiomes_among_marine_grazers_that_feed_on_epilithic_biofilm_communities_/119655
Arboleda Baena, Clara María (2021): PedalMucusEffect_PhotosyntheticBiofilmCoverPercentage. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170586.v1
Arboleda Baena, Clara María (2021): PedalMucus_MANOVA_Carbohydrates/ProteinsContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170523.v1
Arboleda Baena, Clara María (2021): PedalMucus_ProteinsContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170520.v1
Arboleda Baena, Clara María (2021): PedalMucus_CarbohydratesContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170514.v1.
Arboleda Baena, Clara María (2021): GrazerAssemblageBiomass.csv. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15113490.v2.
Arboleda Baena, Clara María (2021): PedalMucus_pH. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15115059.v2 |
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Comisión Nacional de Investigación Científica y Tecnológica (Chile) |
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Comisión Nacional de Investigación Científica y Tecnológica (Chile) Arboleda-Baena, Clara Pareja, Claudia Belén Pla, Isidora Logares, Ramiro De la Iglesia, Rodrigo Navarrete, Sergio A. |
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Mollusk Grazers Epilithic biofilm Intertidal Pedal mucus microbiota |
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Arboleda-Baena, Clara Pareja, Claudia Belén Pla, Isidora Logares, Ramiro De la Iglesia, Rodrigo Navarrete, Sergio A. |
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Arboleda-Baena, Clara |
| title |
Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities |
| title_short |
Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities |
| title_full |
Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities |
| title_fullStr |
Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities |
| title_full_unstemmed |
Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities |
| title_sort |
hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities |
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PeerJ |
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2022-09 |
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http://hdl.handle.net/10261/284479 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100002848 http://dx.doi.org/10.13039/501100009610 |
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1777667883498733568 |
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dig-icm-es-10261-2844792022-12-03T02:43:29Z Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities Arboleda-Baena, Clara Pareja, Claudia Belén Pla, Isidora Logares, Ramiro De la Iglesia, Rodrigo Navarrete, Sergio A. Comisión Nacional de Investigación Científica y Tecnológica (Chile) Agencia Nacional de Investigación y Desarrollo (Chile) Pontificia Universidad Católica de Chile Agencia Estatal de Investigación (España) Mollusk Grazers Epilithic biofilm Intertidal Pedal mucus microbiota 31 pages, 6 figures, supplemental Information https://doi.org/10.7717/peerj.13642.-- DNA Deposition: The following information was supplied regarding the deposition of DNA sequences: The sequence data are available at the European Nucleotide Archive (ENA) database: PRJEB41739 .-- Data Availability: The following information was supplied regarding data availability: The data is available at Figshare: https://figshare.com/projects/Hidden_interactions_in_the_intertidal_rocky_shore_variation_in_pedal_mucus_microbiomes_among_marine_grazers_that_feed_on_epilithic_biofilm_communities_/119655 Arboleda Baena, Clara María (2021): PedalMucusEffect_PhotosyntheticBiofilmCoverPercentage. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170586.v1 Arboleda Baena, Clara María (2021): PedalMucus_MANOVA_Carbohydrates/ProteinsContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170523.v1 Arboleda Baena, Clara María (2021): PedalMucus_ProteinsContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170520.v1 Arboleda Baena, Clara María (2021): PedalMucus_CarbohydratesContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170514.v1. Arboleda Baena, Clara María (2021): GrazerAssemblageBiomass.csv. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15113490.v2. Arboleda Baena, Clara María (2021): PedalMucus_pH. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15115059.v2 In marine ecosystems, most invertebrates possess diverse microbiomes on their external surfaces, such as those found in the pedal mucus of grazing gastropods and chitons that aids displacement on different surfaces. The microbes are then transported around and placed in contact with free-living microbial communities of micro and other macro-organisms, potentially exchanging species and homogenizing microbial composition and structure among grazer hosts. Here, we characterize the microbiota of the pedal mucus of five distantly related mollusk grazers, quantify differences in microbial community structure, mucus protein and carbohydrate content, and, through a simple laboratory experiment, assess their effects on integrated measures of biofilm abundance. Over 665 Amplicon Sequence Variants (ASVs) were found across grazers, with significant differences in abundance and composition among grazer species and epilithic biofilms. The pulmonate limpet Siphonaria lessonii and the periwinkle Echinolittorina peruviana shared similar microbiota. The microbiota of the chiton Chiton granosus, keyhole limpet Fissurella crassa, and scurrinid limpet Scurria araucana differed markedly from one another, and form those of the pulmonate limpet and periwinkle. Flavobacteriaceae (Bacteroidia) and Colwelliaceae (Gammaproteobacteria) were the most common among microbial taxa. Microbial strict specialists were found in only one grazer species. The pedal mucus pH was similar among grazers, but carbohydrate and protein concentrations differed significantly. Yet, differences in mucus composition were not reflected in microbial community structure. Only the pedal mucus of F. crassa and S. lessonii negatively affected the abundance of photosynthetic microorganisms in the biofilm, demonstrating the specificity of the pedal mucus effects on biofilm communities. Thus, the pedal mucus microbiota are distinct among grazer hosts and can affect and interact non-trophically with the epilithic biofilms on which grazers feed, potentially leading to microbial community coalescence mediated by grazer movement. Further studies are needed to unravel the myriad of non-trophic interactions and their reciprocal impacts between macro- and microbial communities Funding for these studies and for international collaboration was provided by CONICYT–National PhD scholarship Program 2016 and CONICYT (Chilean National Agency for Research and Development (ANID)) international complement to CMAB, and by Fondecyt grants No. 1160289 and 1200636 to Sergio Navarrete, and No. 1171259 to Rodrigo De la Iglesia. Complementary funding was provided by Chilean National Agency for Research and Development (ANID) PIA/BASAL FB0002 to Sergio Navarrete. The small grants for undergraduate research involvement, for winter 2018 and summer 2019, was provided by the Pontificia Universidad Católica de Chile With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) Peer reviewed 2022-12-02T12:22:55Z 2022-12-02T12:22:55Z 2022-09 artículo PeerJ 10: e13642 (2022) CEX2019-000928-S http://hdl.handle.net/10261/284479 10.7717/peerj.13642 2167-8359 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100002848 http://dx.doi.org/10.13039/501100009610 en Publisher's version https://doi.org/10.7717/peerj.13642 Sí open PeerJ |