Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254

Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254

20 pages, 1 figure, 6 tables

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Main Authors: Livingstone, David R., Nasci, Cristina, Solé, Montserrat, Da Ros, Lucia, O'Hara, Sean C.M., Peters, Laurence D., Fossato, Valentino, Wootton, A. Nicola, Goldfarb, Peter S.
Format: artículo biblioteca
Published: Elsevier 1997-06
Subjects:Mytilus galloprovincialis, Mussel digestive gland, Cytochrome P4501A, Benzo[a]pyrene metabolism, Polychlorobiphenyls, 2,2′,3,4,4′,5′-Hexachlorobiphenyl, Induction,
Online Access:http://hdl.handle.net/10261/275544
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spelling dig-icm-es-10261-2755442022-07-14T10:46:42Z Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254 Livingstone, David R. Nasci, Cristina Solé, Montserrat Da Ros, Lucia O'Hara, Sean C.M. Peters, Laurence D. Fossato, Valentino Wootton, A. Nicola Goldfarb, Peter S. Mytilus galloprovincialis Mussel digestive gland Cytochrome P4501A Benzo[a]pyrene metabolism Polychlorobiphenyls 2,2′,3,4,4′,5′-Hexachlorobiphenyl Induction 20 pages, 1 figure, 6 tables The induction of a cytochrome P450 with immunochemical similarities to CYP1A, and accompanying changes in microsomal NADPH-dependent benzo[a]pyrene (BaP) metabolism, were examined in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 20 ppb water-borne polychlorobiphenyl (PCB) mixture (Arochlor 1254) for 4 or 10 days, or 4 days after a single injection into the mantle cavity of the mixed-type inducer PCB congener 2,2′,3,4,4′,5′-hexachlorobiphenyl (CB-138; 2.5 μg g−1 wet weight). Whole animal tissue levels of PCB following water-column exposure or injection were similar to those for mussel species from polluted field sites, viz. 0.8 to 1.9 μg g−1 wet weight. Levels of microsomal CYP1A-immunopositive protein increased 59% (CB-138) and 72% (Arochlor 1254; 10 days exposure) as determined by Western blot analysis using polyclonal antibodies to hepatic CYP1A of perch (Perca fluviatilis). No changes were seen in levels of digestive gland CYP1A-like mRNA 4 days after injection of CB-138 as determined by Northern analysis using cDNA to hepatic CYP1A1 of rainbow trout (Oncorhynchus mykiss). The increases in levels of CYP1A-immunopositive protein were accompanied by a shift in microsomal NADPH-dependent BaP metabolism towards phenol and diol and away from dione production, the former increasing from 32 to 85% of total free metabolites. The marked decrease in dione production (which is the major BaP metabolite formed in control microsomes) resulted in no increase in total microsomal BaP metabolism with exposure to PCBs. The Type I ligand α-naphthoflavone markedly inhibited microsomal phenol but had no affect on dione production, whereas the Type II ligand clotrimazole markedly inhibited dione, but had much less effect on phenol production. The overall results are interpreted in terms of the existence of an inducible CYP1A-like enzyme catalysing predominantly 2-electron monooxygenation leading to epoxide (and hence phenol and diol) formation, and a constitutive non-inducible cytochrome P450 catalysing predominantly 1-electron oxidation leading to dione formation. Both Arochlor 1254 or CB-138 produced cellular damage in the digestive gland in the form of decreased epithelial digestive cell height and decreased lysosomal membrane stability 2022-07-14T10:40:03Z 2022-07-14T10:40:03Z 1997-06 2022-07-14T10:40:04Z artículo issn: 0166-445X Aquatic Toxicology 38(4): 205-224 (1997) http://hdl.handle.net/10261/275544 10.1016/S0166-445X(96)00847-8 https://doi.org/10.1016/S0166-445X(96)00847-8 No none Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
topic Mytilus galloprovincialis
Mussel digestive gland
Cytochrome P4501A
Benzo[a]pyrene metabolism
Polychlorobiphenyls
2,2′,3,4,4′,5′-Hexachlorobiphenyl
Induction
Mytilus galloprovincialis
Mussel digestive gland
Cytochrome P4501A
Benzo[a]pyrene metabolism
Polychlorobiphenyls
2,2′,3,4,4′,5′-Hexachlorobiphenyl
Induction
spellingShingle Mytilus galloprovincialis
Mussel digestive gland
Cytochrome P4501A
Benzo[a]pyrene metabolism
Polychlorobiphenyls
2,2′,3,4,4′,5′-Hexachlorobiphenyl
Induction
Mytilus galloprovincialis
Mussel digestive gland
Cytochrome P4501A
Benzo[a]pyrene metabolism
Polychlorobiphenyls
2,2′,3,4,4′,5′-Hexachlorobiphenyl
Induction
Livingstone, David R.
Nasci, Cristina
Solé, Montserrat
Da Ros, Lucia
O'Hara, Sean C.M.
Peters, Laurence D.
Fossato, Valentino
Wootton, A. Nicola
Goldfarb, Peter S.
Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254
description 20 pages, 1 figure, 6 tables
format artículo
topic_facet Mytilus galloprovincialis
Mussel digestive gland
Cytochrome P4501A
Benzo[a]pyrene metabolism
Polychlorobiphenyls
2,2′,3,4,4′,5′-Hexachlorobiphenyl
Induction
author Livingstone, David R.
Nasci, Cristina
Solé, Montserrat
Da Ros, Lucia
O'Hara, Sean C.M.
Peters, Laurence D.
Fossato, Valentino
Wootton, A. Nicola
Goldfarb, Peter S.
author_facet Livingstone, David R.
Nasci, Cristina
Solé, Montserrat
Da Ros, Lucia
O'Hara, Sean C.M.
Peters, Laurence D.
Fossato, Valentino
Wootton, A. Nicola
Goldfarb, Peter S.
author_sort Livingstone, David R.
title Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254
title_short Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254
title_full Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254
title_fullStr Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254
title_full_unstemmed Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and Arochlor 1254
title_sort apparent induction of a cytochrome p450 with immunochemical similarities to cyp1a in digestive gland of the common mussel (mytilus galloprovincialis l.) with exposure to 2,2′,3,4,4′,5′-hexachlorobiphenyl and arochlor 1254
publisher Elsevier
publishDate 1997-06
url http://hdl.handle.net/10261/275544
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