Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation
11 pages, 6 figures, supplementary data https://doi.org/10.1016/j.dci.2020.103848
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2021-01
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| Subjects: | Gonads, Immune, LPS, Methylation, Reproduction, Sex ratio, Zebrafish, |
| Online Access: | http://hdl.handle.net/10261/222028 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 |
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dig-icm-es-10261-2220282022-09-09T08:19:18Z Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation Moraleda, J. Caballero-Huertas, Marta Valdivieso, Alejandro Joly, Sílvia Ji, J. Roher, N. Ribas, Laia Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) European Commission Agencia Estatal de Investigación (España) Gonads Immune LPS Methylation Reproduction Sex ratio Zebrafish 11 pages, 6 figures, supplementary data https://doi.org/10.1016/j.dci.2020.103848 Infections are able to trigger epigenetic modifications; however, epigenetic-mediating infections in the immune system in fish is currently unavailable. Within this purpose, zebrafish were immune-stimulated with three lipopolysaccharides (LPS) during sex differentiation. Methylation patterns of three immune genes were studied by a candidate gene approach together with gene expression analysis, and in adulthood, sex ratios were determined. It was shown that the entrance of LPS was through the gills and accumulated in the pronephros. Significant hypomethylation levels of CASP9 and a significant CpG site for IL1β after Pseudomonas aeruginosa LPS exposure were found. No methylation difference was observed for TNFα. Gene expression and correlation data differed among studied genes. Sex ratios showed a feminization in dose and LPS strain-dependent manner. Here, it is provided epigenetic regulatory mechanisms derived by innate response and the first evidence of possible epigenetic interactions between the immune and reproductive systems This study was mainly supported by the Spanish Ministry of Science and Innovation (MICINN) projects AGL2015-73864-JIN “AMBISEX” together with RyC2018-024017-I and 202030E004 “INTEROMICS” from Spanish National Research Council (CSIC) grants to LR. Further, by Spanish Ministry of Science, European Commission and AGAUR funds to NR (RTI2018-096957-B-C21, MINECO/FEDER and 2014SGR-345 AGAUR) With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI) Peer reviewed 2020-10-30T07:51:57Z 2020-10-30T07:51:57Z 2021-01 artículo http://purl.org/coar/resource_type/c_6501 Developmental & Comparative Immunology 114: 103848 (2021) 0145-305X CEX2019-000928-S http://hdl.handle.net/10261/222028 10.1016/j.dci.2020.103848 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-73864-JIN info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RyC2018-024017-I info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096957-B-C21 Preprint https://doi.org/10.1016/j.dci.2020.103848 Sí open Elsevier |
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| language |
English |
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Gonads Immune LPS Methylation Reproduction Sex ratio Zebrafish Gonads Immune LPS Methylation Reproduction Sex ratio Zebrafish |
| spellingShingle |
Gonads Immune LPS Methylation Reproduction Sex ratio Zebrafish Gonads Immune LPS Methylation Reproduction Sex ratio Zebrafish Moraleda, J. Caballero-Huertas, Marta Valdivieso, Alejandro Joly, Sílvia Ji, J. Roher, N. Ribas, Laia Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation |
| description |
11 pages, 6 figures, supplementary data https://doi.org/10.1016/j.dci.2020.103848 |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Moraleda, J. Caballero-Huertas, Marta Valdivieso, Alejandro Joly, Sílvia Ji, J. Roher, N. Ribas, Laia |
| format |
artículo |
| topic_facet |
Gonads Immune LPS Methylation Reproduction Sex ratio Zebrafish |
| author |
Moraleda, J. Caballero-Huertas, Marta Valdivieso, Alejandro Joly, Sílvia Ji, J. Roher, N. Ribas, Laia |
| author_sort |
Moraleda, J. |
| title |
Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation |
| title_short |
Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation |
| title_full |
Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation |
| title_fullStr |
Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation |
| title_full_unstemmed |
Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation |
| title_sort |
epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation |
| publisher |
Elsevier |
| publishDate |
2021-01 |
| url |
http://hdl.handle.net/10261/222028 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 |
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