Single Cell Genomics Reveals Viruses Consumed by Marine Protists

Brown, Julia M.; Labonté, Jessica M.; Brown, Joseph; Record, Nicholas R.; Poulton, N.J.; Sieracki, Michael E.; Logares, Ramiro; Stepanauskas, R.; National Science Foundation (US)

Single Cell Genomics Reveals Viruses Consumed by Marine Protists

12 pages, 4 figures, 1 table, supplementary material https://www.frontiersin.org/articles/10.3389/fmicb.2020.524828/full#supplementary-material.-- The datasets generated for this study can be found in the OSF https://osf.io/7pm3u/, doi: 10.17605/OSF.IO/7PM3U. Protist SAG assemblies are available through NCBI Bioproject PRJNA655200

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Bibliographic Details
Main Authors:	Brown, Julia M., Labonté, Jessica M., Brown, Joseph, Record, Nicholas R., Poulton, N.J., Sieracki, Michael E., Logares, Ramiro, Stepanauskas, R.
Other Authors:	National Science Foundation (US)
Format:	artículo biblioteca
Language:	English
Published:	Frontiers Media 2020-09
Subjects:	Nanoeukaryote, Marine eukaryote, Virus, Phage, Microbial ecology, Marine food web, Protist,
Online Access:	http://hdl.handle.net/10261/220720 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100000001 http://dx.doi.org/10.13039/501100011033
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spelling	dig-icm-es-10261-2207202021-12-27T15:52:18Z Single Cell Genomics Reveals Viruses Consumed by Marine Protists Brown, Julia M. Labonté, Jessica M. Brown, Joseph Record, Nicholas R. Poulton, N.J. Sieracki, Michael E. Logares, Ramiro Stepanauskas, R. National Science Foundation (US) Ministerio de Economía y Competitividad (España) Research Council of Norway Agencia Estatal de Investigación (España) Nanoeukaryote Marine eukaryote Virus Phage Microbial ecology Marine food web Protist 12 pages, 4 figures, 1 table, supplementary material https://www.frontiersin.org/articles/10.3389/fmicb.2020.524828/full#supplementary-material.-- The datasets generated for this study can be found in the OSF https://osf.io/7pm3u/, doi: 10.17605/OSF.IO/7PM3U. Protist SAG assemblies are available through NCBI Bioproject PRJNA655200 The predominant model of the role of viruses in the marine trophic web is that of the “viral shunt,” where viral infection funnels a substantial fraction of the microbial primary and secondary production back to the pool of dissolved organic matter. Here, we analyzed the composition of non-eukaryotic DNA associated with individual cells of small, planktonic protists in the Gulf of Maine (GoM) and the Mediterranean Sea. We found viral DNA associated with a substantial fraction cells from the GoM (51%) and the Mediterranean Sea (35%). While Mediterranean SAGs contained a larger proportion of cells containing bacterial sequences (49%), a smaller fraction of cells contained bacterial sequences in the GoM (19%). In GoM cells, nearly identical bacteriophage and ssDNA virus sequences where found across diverse lineages of protists, suggesting many of these viruses are non-infective. The fraction of cells containing viral DNA varied among protistan lineages and reached 100% in Picozoa and Choanozoa. These two groups also contained significantly higher numbers of viral sequences than other identified taxa. We consider mechanisms that may explain the presence of viral DNA in protistan cells and conclude that protistan predation on free viral particles contributed to the observed patterns. These findings confirm prior experiments with protistan isolates and indicate that the viral shunt is complemented by a viral link in the marine microbial food web. This link may constitute a sink of viral particles in the ocean and has implications for the flow of carbon through the microbial food web This work was supported by the NSF awards OCE-0623288, OCE-1335810, and OIA-1826734 to RS as well as by the projects INTERACTOMICS (CTM2015-69936-P, MINECO/FEDER, Spain) and MicroEcoSystems (Research Council of Norway 240904, Norway) to RL With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI) Peer reviewed 2020-10-05T08:48:31Z 2020-10-05T08:48:31Z 2020-09 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Microbiology 11: 524828 (2020) CEX2019-000928-S http://hdl.handle.net/10261/220720 10.3389/fmicb.2020.524828 1664-302X http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100000001 http://dx.doi.org/10.13039/501100011033 33072003 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2015-69936-P Publisher's version https://doi.org/10.3389/fmicb.2020.524828 Sí open Frontiers Media
institution	ICM ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-icm-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del ICM España
language	English
topic	Nanoeukaryote Marine eukaryote Virus Phage Microbial ecology Marine food web Protist Nanoeukaryote Marine eukaryote Virus Phage Microbial ecology Marine food web Protist
spellingShingle	Nanoeukaryote Marine eukaryote Virus Phage Microbial ecology Marine food web Protist Nanoeukaryote Marine eukaryote Virus Phage Microbial ecology Marine food web Protist Brown, Julia M. Labonté, Jessica M. Brown, Joseph Record, Nicholas R. Poulton, N.J. Sieracki, Michael E. Logares, Ramiro Stepanauskas, R. Single Cell Genomics Reveals Viruses Consumed by Marine Protists
description	12 pages, 4 figures, 1 table, supplementary material https://www.frontiersin.org/articles/10.3389/fmicb.2020.524828/full#supplementary-material.-- The datasets generated for this study can be found in the OSF https://osf.io/7pm3u/, doi: 10.17605/OSF.IO/7PM3U. Protist SAG assemblies are available through NCBI Bioproject PRJNA655200
author2	National Science Foundation (US)
author_facet	National Science Foundation (US) Brown, Julia M. Labonté, Jessica M. Brown, Joseph Record, Nicholas R. Poulton, N.J. Sieracki, Michael E. Logares, Ramiro Stepanauskas, R.
format	artículo
topic_facet	Nanoeukaryote Marine eukaryote Virus Phage Microbial ecology Marine food web Protist
author	Brown, Julia M. Labonté, Jessica M. Brown, Joseph Record, Nicholas R. Poulton, N.J. Sieracki, Michael E. Logares, Ramiro Stepanauskas, R.
author_sort	Brown, Julia M.
title	Single Cell Genomics Reveals Viruses Consumed by Marine Protists
title_short	Single Cell Genomics Reveals Viruses Consumed by Marine Protists
title_full	Single Cell Genomics Reveals Viruses Consumed by Marine Protists
title_fullStr	Single Cell Genomics Reveals Viruses Consumed by Marine Protists
title_full_unstemmed	Single Cell Genomics Reveals Viruses Consumed by Marine Protists
title_sort	single cell genomics reveals viruses consumed by marine protists
publisher	Frontiers Media
publishDate	2020-09
url	http://hdl.handle.net/10261/220720 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100000001 http://dx.doi.org/10.13039/501100011033
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_version_	1777667263289098240

Single Cell Genomics Reveals Viruses Consumed by Marine Protists

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