Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay
Special issue Phytoplankton Life-Cycles and Their Impacts on the Ecology of Harmful Algal Bloom.-- 13 pages, 4 figures, 6 tables
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| Main Authors: | , , , , , , , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2010-02
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| Subjects: | HAB species, Mediterranean Sea, PCR, Ribosomal genes, Resting stages, Sediments, HAB, |
| Online Access: | http://hdl.handle.net/10261/21772 |
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dig-icm-es-10261-217722020-11-26T09:00:09Z Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay Penna, Antonella Battocchi, Cecilia Garcés, Esther Anglès, Silvia Cucchiari, Emellina Totti, Cecilia Kremp, Anke Satta, Cecilia Teodora Giacobbe, Maria Grazia Bravo, Isabel Bastianini, Mauro HAB species Mediterranean Sea PCR Ribosomal genes Resting stages Sediments HAB Special issue Phytoplankton Life-Cycles and Their Impacts on the Ecology of Harmful Algal Bloom.-- 13 pages, 4 figures, 6 tables A PCR-based assay was developed and applied to sediment and sediment trap samples for the detection of different cysts belonging to dinoflagellates and raphidophytes in European coastal areas. Oligonucleotide primers were designed based on the ITS-5.8S and LSU ribosomal gene sequences. The specificity and sensitivity of the PCR assay were assessed using genomic DNA from clonal cultures, plasmid copy number of cloned target sequences, as well as from sediment samples. Qualitative PCR determinations of different cysts in sediment and sediment trap samples were compared to taxonomic examinations by light microscopy. This molecular methodology permitted a fast and specific detection of target cysts in sediment samples. We also detected dinoflagellate and raphidophyte cysts at concentrations not detectable by microscopic methods or that are difficult to identify. The results given by molecular and microscopic methods were comparable. However, higher values of positive detection for target cysts were obtained by PCR than with microscopy. Some taxa were detected in 100% of the samples using PCR, while others were only found in 10% of the samples. The data obtained in this study showed that the PCR-based method is a valid tool for cyst identification in marine sediments This study was financed by the EU funded Research Project SEED (GOCE-CT-2005-003875). E. Garcés was sustained by a Ramon y Cajal contract from the Spanish Ministry of Science and Education Peer reviewed 2010-03-01T13:13:42Z 2010-03-01T13:13:42Z 2010-02 artículo http://purl.org/coar/resource_type/c_6501 Deep Sea Research - Part II - Topical Studies in Oceanography 57(3-4): 288-300 (2010) 0967-0645 http://hdl.handle.net/10261/21772 10.1016/j.dsr2.2009.09.010 en https://doi.org/10.1016/j.dsr2.2009.09.010 none 5875 bytes application/pdf Elsevier |
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biblioteca |
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Europa del Sur |
| libraryname |
Biblioteca del ICM España |
| language |
English |
| topic |
HAB species Mediterranean Sea PCR Ribosomal genes Resting stages Sediments HAB HAB species Mediterranean Sea PCR Ribosomal genes Resting stages Sediments HAB |
| spellingShingle |
HAB species Mediterranean Sea PCR Ribosomal genes Resting stages Sediments HAB HAB species Mediterranean Sea PCR Ribosomal genes Resting stages Sediments HAB Penna, Antonella Battocchi, Cecilia Garcés, Esther Anglès, Silvia Cucchiari, Emellina Totti, Cecilia Kremp, Anke Satta, Cecilia Teodora Giacobbe, Maria Grazia Bravo, Isabel Bastianini, Mauro Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay |
| description |
Special issue Phytoplankton Life-Cycles and Their Impacts on the Ecology of Harmful Algal Bloom.-- 13 pages, 4 figures, 6 tables |
| format |
artículo |
| topic_facet |
HAB species Mediterranean Sea PCR Ribosomal genes Resting stages Sediments HAB |
| author |
Penna, Antonella Battocchi, Cecilia Garcés, Esther Anglès, Silvia Cucchiari, Emellina Totti, Cecilia Kremp, Anke Satta, Cecilia Teodora Giacobbe, Maria Grazia Bravo, Isabel Bastianini, Mauro |
| author_facet |
Penna, Antonella Battocchi, Cecilia Garcés, Esther Anglès, Silvia Cucchiari, Emellina Totti, Cecilia Kremp, Anke Satta, Cecilia Teodora Giacobbe, Maria Grazia Bravo, Isabel Bastianini, Mauro |
| author_sort |
Penna, Antonella |
| title |
Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay |
| title_short |
Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay |
| title_full |
Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay |
| title_fullStr |
Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay |
| title_full_unstemmed |
Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay |
| title_sort |
detection of microalgal resting cysts in european coastal sediments using a pcr-based assay |
| publisher |
Elsevier |
| publishDate |
2010-02 |
| url |
http://hdl.handle.net/10261/21772 |
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