In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males

In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males

12th Congress of the Iberian Association of Comparative Endocrinology (AIEC), 26-28 September 2019, Faro, Portugal

Saved in:
Bibliographic Details
Main Authors: Medina, Paula, Joly, Sílvia, Gómez, Ana, Blázquez, Mercedes
Format: comunicación de congreso biblioteca
Published: Asociación Ibérica de Endocrinología Comparada 2019-09-26
Online Access:http://hdl.handle.net/10261/205269
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-icm-es-10261-205269
record_format koha
spelling dig-icm-es-10261-2052692020-06-17T10:51:25Z In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males Medina, Paula Joly, Sílvia Gómez, Ana Blázquez, Mercedes 12th Congress of the Iberian Association of Comparative Endocrinology (AIEC), 26-28 September 2019, Faro, Portugal In mouse, chicken and newts, RA has been established as a paracrine signal involved in the onset of meiosis, however, in fish, the role of RA in this process is still far from being understood. To gain a better understanding of the influence of RA in the onset of meiosis of the European sea bass, we have studied the in vitro functional responses to the RA signaling pathway agonist, all-trans retinoic acid (atRA), and to the RA synthase inhibitor (aldh inhibitor) 4-diethylaminobenzaldehyde (DEAB) in two meiosis scenarios. The first one is represented by an increase of 11-ketotestosterone (11KT) that takes place during the transition from immature differentiated testis to early recrudescent testis. The second one is represented by an increase of follicle-stimulating hormone (Fsh) that takes place during early stages of meiosis. Among the studied genes, we focused our attention on those involved in RA synthesis (aldh1a2 and aldh1a3) and degradation (cyp26a1). The results show gene expression responses the increase of exogenous RA and to the different meiosis scenarios, confirming the functionality of the RA signaling pathway during this developmental stage. In particular, the upregulation of cyp26a1 as a result of increased RA levels, suggest that it could be used as a suitable molecular marker for the onset of meiosis in European sea bass males 2020-03-26T10:36:18Z 2020-03-26T10:36:18Z 2019-09-26 2020-03-26T10:36:18Z comunicación de congreso http://purl.org/coar/resource_type/c_5794 12th Congress of the Iberian Association of Comparative Endocrinology (2019) http://hdl.handle.net/10261/205269 Sí none Asociación Ibérica de Endocrinología Comparada
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
description 12th Congress of the Iberian Association of Comparative Endocrinology (AIEC), 26-28 September 2019, Faro, Portugal
format comunicación de congreso
author Medina, Paula
Joly, Sílvia
Gómez, Ana
Blázquez, Mercedes
spellingShingle Medina, Paula
Joly, Sílvia
Gómez, Ana
Blázquez, Mercedes
In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males
author_facet Medina, Paula
Joly, Sílvia
Gómez, Ana
Blázquez, Mercedes
author_sort Medina, Paula
title In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males
title_short In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males
title_full In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males
title_fullStr In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males
title_full_unstemmed In vitro functional analysis of the expression of RA signaling pathway genes during meiosis in European sea bass males
title_sort in vitro functional analysis of the expression of ra signaling pathway genes during meiosis in european sea bass males
publisher Asociación Ibérica de Endocrinología Comparada
publishDate 2019-09-26
url http://hdl.handle.net/10261/205269
work_keys_str_mv AT medinapaula invitrofunctionalanalysisoftheexpressionofrasignalingpathwaygenesduringmeiosisineuropeanseabassmales
AT jolysilvia invitrofunctionalanalysisoftheexpressionofrasignalingpathwaygenesduringmeiosisineuropeanseabassmales
AT gomezana invitrofunctionalanalysisoftheexpressionofrasignalingpathwaygenesduringmeiosisineuropeanseabassmales
AT blazquezmercedes invitrofunctionalanalysisoftheexpressionofrasignalingpathwaygenesduringmeiosisineuropeanseabassmales
_version_ 1777667159067983872

Similar Items