A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass

A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass

11 pages, 4 figures, 3 tables, supporting information https://dx.doi.org/10.1111/1755-0998.13111.-- All data related to this publication are publicly available: mapped reads and methylation values of each CpG for each sample can be found in https://doi.org/10.5061/dryad.m0cfxpnz4 Anastasiadi & Piferrer, 2019)

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Bibliographic Details
Main Authors: Anastasiadi, Dafni, Piferrer, Francesc
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Published: Blackwell Publishing 2020-03
Online Access:http://hdl.handle.net/10261/203352
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100011033
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spelling dig-icm-es-10261-2033522021-05-21T07:43:38Z A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass Anastasiadi, Dafni Piferrer, Francesc Ministerio de Economía y Competitividad (España) Agencia Estatal de Investigación (España) 11 pages, 4 figures, 3 tables, supporting information https://dx.doi.org/10.1111/1755-0998.13111.-- All data related to this publication are publicly available: mapped reads and methylation values of each CpG for each sample can be found in https://doi.org/10.5061/dryad.m0cfxpnz4 Anastasiadi & Piferrer, 2019) Age‐related changes in DNA methylation do occur. Taking advantage of this, mammalian and avian epigenetic clocks have been constructed to predict age. In fish, studies on age‐related DNA methylation changes are scarce and no epigenetic clocks have been constructed. However, in fisheries and population dynamics studies there is a need for accurate estimation of age, something that is often impossible for some economically important species with the currently available methods. Here, we used the European sea bass, a marine fish the age of which can be determined with accuracy, to construct a piscine epigenetic clock, the first one in a cold‐blooded vertebrate. We used targeted bisulfite sequencing to amplify 48 CpGs from four genes in muscle samples and applied penalized regressions to predict age. We thus developed an age predictor in fish that is highly accurate (0.824) and precise (2.149 years). In juvenile fish, accelerated growth due to elevated temperatures had no effect on age prediction, indicating that the clock is able to predict the chronological age independently of environmentally‐driven perturbations. An epigenetic clock developed using muscle samples accurately predicted age in samples of testis but not ovaries, possibly reflecting the reproductive biology of fish. In conclusion, we report the development of the first piscine epigenetic clock, paving the way for similar studies in other species. Piscine epigenetic clocks should be of great utility for fisheries management and conservation purposes, where age determination is of crucial D.A. was supported by a PhD scholarship from the Spanish Government (BES–2011–044860). Research was supported by Spanish Ministry of Economy and Competitiveness “Epimark” (ref. AGL2016-78710-R) to F.P. With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI) 2020-03-10T09:07:07Z 2020-03-10T09:07:07Z 2020-03 2020-03-10T09:07:07Z artículo http://purl.org/coar/resource_type/c_6501 issn: 1755-098X issn: 1755-099 Molecular Ecology Resources 20(2): 387-397 (2020) CEX2019-000928-S http://hdl.handle.net/10261/203352 10.1111/1755-0998.13111 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100011033 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-78710-R Preprint https://dx.doi.org/10.1111/1755-0998.13111 Sí open Blackwell Publishing
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
description 11 pages, 4 figures, 3 tables, supporting information https://dx.doi.org/10.1111/1755-0998.13111.-- All data related to this publication are publicly available: mapped reads and methylation values of each CpG for each sample can be found in https://doi.org/10.5061/dryad.m0cfxpnz4 Anastasiadi & Piferrer, 2019)
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Anastasiadi, Dafni
Piferrer, Francesc
format artículo
author Anastasiadi, Dafni
Piferrer, Francesc
spellingShingle Anastasiadi, Dafni
Piferrer, Francesc
A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass
author_sort Anastasiadi, Dafni
title A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass
title_short A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass
title_full A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass
title_fullStr A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass
title_full_unstemmed A clockwork fish: Age prediction using DNA methylation‐based biomarkers in the European seabass
title_sort clockwork fish: age prediction using dna methylation‐based biomarkers in the european seabass
publisher Blackwell Publishing
publishDate 2020-03
url http://hdl.handle.net/10261/203352
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100011033
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