Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets

Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets

12 pages, 3 figures, 2 tables

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Main Authors: Fernández-Díaz, C., Kopecka, Justyna, Cañavate, J. Pedro, Sarasquete, Carmen, Solé, Montserrat
Format: artículo biblioteca
Language:English
Published: Elsevier 2006-02-28
Subjects:Microcapsules, Vitamin A, Antioxidant enzymes, Lipid peroxidation, Reactive oxygen species, Stress proteins, Histology, Solea senegalensis, Larvae,
Online Access:http://hdl.handle.net/10261/20326
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spelling dig-icm-es-10261-203262020-11-12T08:20:50Z Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets Fernández-Díaz, C. Kopecka, Justyna Cañavate, J. Pedro Sarasquete, Carmen Solé, Montserrat Microcapsules Vitamin A Antioxidant enzymes Lipid peroxidation Reactive oxygen species Stress proteins Histology Solea senegalensis Larvae 12 pages, 3 figures, 2 tables Growth, development, antioxidant enzymes, stress proteins (HSP70 and HSP60), lipid peroxidation (LP) and histology in Solea senegalensis larvae were followed from 8 to 30 days post hatching (dph). Larvae were fed on three different diets: (1) live Artemia nauplii, (2) microcapsules elaborated by internal gelation, (MA) and (3) these same microcapsules but 10-fold supplemented with vitamin A (MAV). The Artemia fed group showed higher growth and a faster metamorphosis than the ones fed with microencapsulated diets, although all had similarly high survival rates of 80%. Vitamin A (VA) supplementation improved growth and development from 15 dph in relation to the strictly inert diet (MA). Larvae fed with Artemia showed organs and tissues with a normal pattern of development, whereas histological alterations were seen in larvae fed with both inert diets. The antioxidant enzymes: catalase (KAT), superoxide dismutase (SOD), and total glutathione peroxidase (t-GPX) as well as LP levels and stress proteins (HSP70 but not HSP60), measured in whole larvae, showed diet and age dependence in their response. Larvae fed with both inert diets showed similar biomarker activities, but these activities were different ( p b0.05) from larvae fed with Artemia. That is, KAT and HSP70 were lower in larvae fed with live prey and t-GPX and LP levels were lower in larvae fed with the inert food. Among the factors responsible for increased antioxidant defenses were the initiation of metamorphosis and the use of inert food. This study suggests the usefulness of the biomarkers selected as tools to evaluate the effects of compound diets on larvae. The Ministry of Science and Technology, Spain (MCYT project AGL2003-03558) supported this work. A grant to J. Kopecka through CSIC-PAN collaboration is also acknowledged. Peer reviewed 2010-01-26T11:24:52Z 2010-01-26T11:24:52Z 2006-02-28 artículo http://purl.org/coar/resource_type/c_6501 Aquaculture 251(2-4): 573–584 (2006) 0044-8486 http://hdl.handle.net/10261/20326 10.1016/j.aquaculture.2005.06.014 en https://doi.org/10.1016/j.aquaculture.2005.06.014 none 22195 bytes application/pdf Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Microcapsules
Vitamin A
Antioxidant enzymes
Lipid peroxidation
Reactive oxygen species
Stress proteins
Histology
Solea senegalensis
Larvae
Microcapsules
Vitamin A
Antioxidant enzymes
Lipid peroxidation
Reactive oxygen species
Stress proteins
Histology
Solea senegalensis
Larvae
spellingShingle Microcapsules
Vitamin A
Antioxidant enzymes
Lipid peroxidation
Reactive oxygen species
Stress proteins
Histology
Solea senegalensis
Larvae
Microcapsules
Vitamin A
Antioxidant enzymes
Lipid peroxidation
Reactive oxygen species
Stress proteins
Histology
Solea senegalensis
Larvae
Fernández-Díaz, C.
Kopecka, Justyna
Cañavate, J. Pedro
Sarasquete, Carmen
Solé, Montserrat
Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets
description 12 pages, 3 figures, 2 tables
format artículo
topic_facet Microcapsules
Vitamin A
Antioxidant enzymes
Lipid peroxidation
Reactive oxygen species
Stress proteins
Histology
Solea senegalensis
Larvae
author Fernández-Díaz, C.
Kopecka, Justyna
Cañavate, J. Pedro
Sarasquete, Carmen
Solé, Montserrat
author_facet Fernández-Díaz, C.
Kopecka, Justyna
Cañavate, J. Pedro
Sarasquete, Carmen
Solé, Montserrat
author_sort Fernández-Díaz, C.
title Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets
title_short Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets
title_full Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets
title_fullStr Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets
title_full_unstemmed Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets
title_sort variations on development and stress defences in solea senegalensis larvae fed on live and microencapsulated diets
publisher Elsevier
publishDate 2006-02-28
url http://hdl.handle.net/10261/20326
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AT canavatejpedro variationsondevelopmentandstressdefencesinsoleasenegalensislarvaefedonliveandmicroencapsulateddiets
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