Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions

Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions

10 pages, 5 figures, 1 table

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Bibliographic Details
Main Authors: Llaveria, Gisela, Figueroa, Rosa Isabel, Garcés, Esther, Berdalet, Elisa
Format: artículo biblioteca
Language:English
Published: Blackwell Publishing 2009-09
Subjects:Alexandrium minutum, Cell cycle, Coulter counter, Dinoflagellates, Flow cytometry, G2/Mitosis, Mortality, Small-scale turbulence,
Online Access:http://hdl.handle.net/10261/18203
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spelling dig-icm-es-10261-182032022-12-14T10:23:39Z Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions Llaveria, Gisela Figueroa, Rosa Isabel Garcés, Esther Berdalet, Elisa Alexandrium minutum Cell cycle Coulter counter Dinoflagellates Flow cytometry G2/Mitosis Mortality Small-scale turbulence 10 pages, 5 figures, 1 table Decreased net population growth rates and cellular abundances have been observed in dinoflagellate species exposed to small-scale turbulence. Here, we investigated whether these effects were caused by alterations in the cell cycle and/or by cell mortality and, in turn, whether these two mechanisms depended on the duration of exposure to turbulence. The study was conducted on the toxic dinoflagellate Alexandrium minutum Halim, with the same experimental design and setup used in previous studies to allow direct comparison among results. A combination of microscopy and Coulter Counter measurements allowed us to detect cell mortality, based on the biovolume of broken cells and thecae. The turbulence applied during the exponential growth phase caused an immediate transitory arrest in the G2/M phase, but significant mortality did not occur. This finding suggests that high intensities of small-scale turbulence can alter the cell division, likely affecting the correct chromosome segregation during the dinomitosis. When shaking persisted for >4 d, mortality signals and presence of anomalously swollen cells appeared, hinting at the activation of mechanisms that induce programmed cell death. Our study suggests that the sensitivity of dinoflagellates to turbulence may drive these organisms to find the most favorable (calm) conditions to complete their division cycle. This work has been supported by the Spanish funded projects TURDITOX (CTM2005-03547/MAR), TURECOTOX (CTM2006-13884-C02-00/MAR) and by the EU project SEED (GOCE-CT-2005-003875) Peer reviewed 2009-10-30T10:57:59Z 2009-10-30T10:57:59Z 2009-09 artículo http://purl.org/coar/resource_type/c_6501 Journal of Phycology 45(5): 1106-1115 (2009) 0022-3646 http://hdl.handle.net/10261/18203 10.1111/j.1529-8817.2009.00740.x en https://doi.org/10.1111/j.1529-8817.2009.00740.x none 8985 bytes application/pdf Blackwell Publishing
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Alexandrium minutum
Cell cycle
Coulter counter
Dinoflagellates
Flow cytometry
G2/Mitosis
Mortality
Small-scale turbulence
Alexandrium minutum
Cell cycle
Coulter counter
Dinoflagellates
Flow cytometry
G2/Mitosis
Mortality
Small-scale turbulence
spellingShingle Alexandrium minutum
Cell cycle
Coulter counter
Dinoflagellates
Flow cytometry
G2/Mitosis
Mortality
Small-scale turbulence
Alexandrium minutum
Cell cycle
Coulter counter
Dinoflagellates
Flow cytometry
G2/Mitosis
Mortality
Small-scale turbulence
Llaveria, Gisela
Figueroa, Rosa Isabel
Garcés, Esther
Berdalet, Elisa
Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions
description 10 pages, 5 figures, 1 table
format artículo
topic_facet Alexandrium minutum
Cell cycle
Coulter counter
Dinoflagellates
Flow cytometry
G2/Mitosis
Mortality
Small-scale turbulence
author Llaveria, Gisela
Figueroa, Rosa Isabel
Garcés, Esther
Berdalet, Elisa
author_facet Llaveria, Gisela
Figueroa, Rosa Isabel
Garcés, Esther
Berdalet, Elisa
author_sort Llaveria, Gisela
title Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions
title_short Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions
title_full Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions
title_fullStr Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions
title_full_unstemmed Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions
title_sort cell cycle and cell mortality of alexandrium minutum (dinophyceae) under small-scale turbulence conditions
publisher Blackwell Publishing
publishDate 2009-09
url http://hdl.handle.net/10261/18203
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