Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass)
VII Jornada de Cromatina i Epigenètica organizada por la Secció de Cromatina i Epigenètica de la Societat Catalana de Biologia (SCB) con el Barcelona Chromatin Club (BCC).-- 1 page
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Societat Catalana de Biologia
2017-03-24
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| Online Access: | http://hdl.handle.net/10261/177696 http://dx.doi.org/10.13039/501100003329 |
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dig-icm-es-10261-1776962019-03-13T01:58:06Z Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass) Anastasiadi, Dafni Piferrer, Francesc Ministerio de Economía y Competitividad (España) VII Jornada de Cromatina i Epigenètica organizada por la Secció de Cromatina i Epigenètica de la Societat Catalana de Biologia (SCB) con el Barcelona Chromatin Club (BCC).-- 1 page In animals, age-related changes in DNA methylation occur. In general, there is global genomic hypomethylation accompanied by CpG-specific hypermethylation as a result of intrinsic and extrinsic influences. Mammalian epigenetic clocks have been suggested and used that estimate the biological age independently of the tissue tested. In fish, ageing studies based on epigenetic changes are scarce, while in fisheries and fish population studies there is a need for accurate estimation of age for which epigenetic biomarkers can be candidates. Here, we suggest CpG loci exhibiting decreasing or increasing methylation with age as potential piscine biomarkers of age. We used individuals from three different age classes comprising the reproductively immature and the senescent phase of a marine teleost fish, the European sea bass, to inquire the methylation status of the regulatory regions of important genes. For this inquiry, we successfully developed a low-cost high-resolution PCR-based NGS protocol applied in 22 genes and 36 samples. We found gene-specific ranges of DNA methylation that were affected by tissue identity and age. Furthermore, we fitted the elastic net regularization paths for generalized linear model in order to identify the minimum CpG number sufficient to predict the fish age. A minimum of 10 CpGs, out of 301 tested in total (3.3%), distributed in 6 genes, were enough to explain the age of the fish. Importantly, CpGs with clear increasing or decreasing tendencies in DNA methylation with age were detected and the youngest fish mean methylation matched with ~1 year old fish from an independent experiment. The sum of the suggested CpGs can serve as a guide to focus the efforts for the development of an accurate intertissue piscine epigenetic clock Supported by MINECO grant AGL2013-41047-R “Epifarm” to FP Peer Reviewed 2019-03-12T07:30:47Z 2019-03-12T07:30:47Z 2017-03-24 2019-03-12T07:30:47Z comunicación de congreso http://purl.org/coar/resource_type/c_5794 VII Jornada de Cromatina i Epigenètica (2017) http://hdl.handle.net/10261/177696 http://dx.doi.org/10.13039/501100003329 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-41047-R Sí open Societat Catalana de Biologia |
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VII Jornada de Cromatina i Epigenètica organizada por la Secció de Cromatina i Epigenètica de la Societat Catalana de Biologia (SCB) con el Barcelona Chromatin Club (BCC).-- 1 page |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Anastasiadi, Dafni Piferrer, Francesc |
| format |
comunicación de congreso |
| author |
Anastasiadi, Dafni Piferrer, Francesc |
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Anastasiadi, Dafni Piferrer, Francesc Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass) |
| author_sort |
Anastasiadi, Dafni |
| title |
Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass) |
| title_short |
Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass) |
| title_full |
Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass) |
| title_fullStr |
Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass) |
| title_full_unstemmed |
Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass) |
| title_sort |
age-related dna methylation changes in somatic and gonadal tissues of teleost fish (european sea bass) |
| publisher |
Societat Catalana de Biologia |
| publishDate |
2017-03-24 |
| url |
http://hdl.handle.net/10261/177696 http://dx.doi.org/10.13039/501100003329 |
| work_keys_str_mv |
AT anastasiadidafni agerelateddnamethylationchangesinsomaticandgonadaltissuesofteleostfisheuropeanseabass AT piferrerfrancesc agerelateddnamethylationchangesinsomaticandgonadaltissuesofteleostfisheuropeanseabass |
| _version_ |
1777666875742748672 |