Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells
9 pages, 7 figures
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
|
| Subjects: | TGF-α, IGFBP-4, IGFBP-4 Protease, Granulosa cells, Protein expression, Hormone inhibition, Transforming growth factor alpha, Somatomedin binding protein 4, Follitropin, IGFBP, TGF, |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-icm-es-10261-16832 |
|---|---|
| record_format |
koha |
| spelling |
dig-icm-es-10261-168322020-11-26T11:18:00Z Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells Piferrer, Francesc Li, D. Shimasaki, S. Erickson, G.F. TGF-α IGFBP-4 IGFBP-4 Protease Granulosa cells Protein expression Hormone inhibition Transforming growth factor alpha Somatomedin binding protein 4 Follitropin IGFBP TGF 9 pages, 7 figures The ability of TGF-α to regulate insulin-like growth factor binding protein-4 (IGFBP-4), was investigated. Primary cultures of rat granulosa cells (GC) were grown in serum-free medium with rat (r) TGF-α and/or rFSH, and secreted IGFBP-4 protein and its steady state mRNA levels were measured by Western immunoblotting and Northern blotting, respectively. Control (untreated) cells secreted IGFBP-4 spontaneously, and the levels were increased by rTGF-α in a dose- and time-dependent manner. rTGF-α abolished FSH-induced IGFBP-4 protease activity and suppressed FSH-dependent effects on IGFBP-4 production. IGFBP-4 mRNA levels were decreased and increased by FSH and TGF-α, respectively, and TGF-α blocked the FSH effects. These results demonstrate that TGF-α is a potent stimulator of IGFBP-4 expression in rat GC and can overcome the regulatory effects of FSH on IGFBP-4 production. The consequence of these TGF-α effects is a marked, sustained increase in the levels of IGFBP-4 in the microenvironment This work was supported by NICHD Grants HD-29008 and HD-30308. F.P. was supported by a NATO postdoctoral fellowship Peer reviewed 1997-09-30 artículo http://purl.org/coar/resource_type/c_6501 Molecular and Cellular Endocrinology 133(1): 9-17 (1997) 0303-7207 10261/16832 10.1016/S0303-7207(97)00139-1 en https://doi.org/10.1016/S0303-7207(97)00139-1 none Elsevier |
| institution |
ICM ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-icm-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del ICM España |
| language |
English |
| topic |
TGF-α IGFBP-4 IGFBP-4 Protease Granulosa cells Protein expression Hormone inhibition Transforming growth factor alpha Somatomedin binding protein 4 Follitropin IGFBP TGF TGF-α IGFBP-4 IGFBP-4 Protease Granulosa cells Protein expression Hormone inhibition Transforming growth factor alpha Somatomedin binding protein 4 Follitropin IGFBP TGF |
| spellingShingle |
TGF-α IGFBP-4 IGFBP-4 Protease Granulosa cells Protein expression Hormone inhibition Transforming growth factor alpha Somatomedin binding protein 4 Follitropin IGFBP TGF TGF-α IGFBP-4 IGFBP-4 Protease Granulosa cells Protein expression Hormone inhibition Transforming growth factor alpha Somatomedin binding protein 4 Follitropin IGFBP TGF Piferrer, Francesc Li, D. Shimasaki, S. Erickson, G.F. Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells |
| description |
9 pages, 7 figures |
| format |
artículo |
| topic_facet |
TGF-α IGFBP-4 IGFBP-4 Protease Granulosa cells Protein expression Hormone inhibition Transforming growth factor alpha Somatomedin binding protein 4 Follitropin IGFBP TGF |
| author |
Piferrer, Francesc Li, D. Shimasaki, S. Erickson, G.F. |
| author_facet |
Piferrer, Francesc Li, D. Shimasaki, S. Erickson, G.F. |
| author_sort |
Piferrer, Francesc |
| title |
Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells |
| title_short |
Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells |
| title_full |
Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells |
| title_fullStr |
Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells |
| title_full_unstemmed |
Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells |
| title_sort |
transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (igfbp-4) expression and blocks follicle-stimulating hormone regulation of igfbp-4 production in rat granulosa cells |
| publisher |
Elsevier |
| work_keys_str_mv |
AT piferrerfrancesc transforminggrowthfactorastimulatesinsulinlikegrowthfactorbindingprotein4igfbp4expressionandblocksfolliclestimulatinghormoneregulationofigfbp4productioninratgranulosacells AT lid transforminggrowthfactorastimulatesinsulinlikegrowthfactorbindingprotein4igfbp4expressionandblocksfolliclestimulatinghormoneregulationofigfbp4productioninratgranulosacells AT shimasakis transforminggrowthfactorastimulatesinsulinlikegrowthfactorbindingprotein4igfbp4expressionandblocksfolliclestimulatinghormoneregulationofigfbp4productioninratgranulosacells AT ericksongf transforminggrowthfactorastimulatesinsulinlikegrowthfactorbindingprotein4igfbp4expressionandblocksfolliclestimulatinghormoneregulationofigfbp4productioninratgranulosacells |
| _version_ |
1777665688888934400 |