Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)

Blázquez, Mercedes; Piferrer, Francesc

Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)

12 pages, 5 figures

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Bibliographic Details
Main Authors:	Blázquez, Mercedes, Piferrer, Francesc
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2004-02-26
Subjects:	Cytochrome P450 aromatase, Brain, Sex steroids, Neuroestrogens, Sex differentiation, Sea bass, Dicentrarchus labrax,
Online Access:	http://hdl.handle.net/10261/16828
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spelling	dig-icm-es-10261-168282020-11-26T13:10:11Z Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax) Blázquez, Mercedes Piferrer, Francesc Cytochrome P450 aromatase Brain Sex steroids Neuroestrogens Sex differentiation Sea bass Dicentrarchus labrax 12 pages, 5 figures The teleost brain is characterized by exceptionally high levels of aromatase, the enzyme that converts androgens into estrogens, and by its continuous growth throughout life. Gonadal estrogens have been implicated in sex differentiation and the control of reproduction in adult fish, but the role of neural estrogens during early development is far from clear. The present study describes the isolation and characterization of the cDNA sequence from brain aromatase (P450aromB) in the European sea bass (Dicentrarchus labrax L.), a well established model for neuroendocrine research in fish. P450aromB was cloned from a brain cDNA library and encoded a predicted protein of 505 residues, with a calculated molecular weight of 57.2 kDa. Comparisons of the deduced amino acid sequence to that of the ovarian aromatase (P450aromA) in the same species revealed 62% identity, lower than the 84% identity shared between sea bass and tilapia brain aromatases. Phylogenetic analysis showed the occurrence of a gene duplication for P450arom in the teleost lineage after its divergence from the tetrapods. Moreover, the low percentage of identity between brain and ovarian forms within the same species suggests that both genes evolved separately right after the appearance of the teleosts. Tissue-specific expression of P450aromA and P450aromB mRNA was studied in adult sea bass. P450aromB was preferentially expressed in brain of both males and females but also present at much lower levels in testis, ovary and head kidney, an organ known for its steroidogenic capabilities in fish. However, P450aromA expression was restricted to testis and ovary. A semiquantitative PCR was developed to measure P450aromB mRNA levels. Analysis of the expression of P450aromB in the brain of juvenile sea bass showed that females exhibited higher mRNA levels than males at 200 days post fertilization (dpf), by the time of gonadal sex differentiation. A switch in expression occurred thereafter, between 200 and 250 dpf, with males exhibiting higher levels than females. This situation was maintained by 300 dpf and is in agreement with measured levels of enzymatic activity in adults. These changes and sex-related differences in expression profiles may imply differences in the functionality of the enzyme between males and females, suggesting an important role for P450aromB in sea bass sex differentiation. However, due to the continuous growth of the teleost brain throughout life, a role in neurogenesis for brain aromatase should also be considered This work was supported by E.U. grant Q5RS-2000-31365 to F.P. M.B. was supported by a postdoctoral contract from the E.U. Peer reviewed 2009-09-10T10:06:16Z 2009-09-10T10:06:16Z 2004-02-26 artículo http://purl.org/coar/resource_type/c_6501 Molecular and Cellular Endocrinology 219(1-2): 83-94 (2004) 0303-7207 http://hdl.handle.net/10261/16828 10.1016/j.mce.2004.01.006 en https://doi.org/10.1016/j.mce.2004.01.006 none 918459 bytes application/pdf Elsevier
institution	ICM ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-icm-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del ICM España
language	English
topic	Cytochrome P450 aromatase Brain Sex steroids Neuroestrogens Sex differentiation Sea bass Dicentrarchus labrax Cytochrome P450 aromatase Brain Sex steroids Neuroestrogens Sex differentiation Sea bass Dicentrarchus labrax
spellingShingle	Cytochrome P450 aromatase Brain Sex steroids Neuroestrogens Sex differentiation Sea bass Dicentrarchus labrax Cytochrome P450 aromatase Brain Sex steroids Neuroestrogens Sex differentiation Sea bass Dicentrarchus labrax Blázquez, Mercedes Piferrer, Francesc Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)
description	12 pages, 5 figures
format	artículo
topic_facet	Cytochrome P450 aromatase Brain Sex steroids Neuroestrogens Sex differentiation Sea bass Dicentrarchus labrax
author	Blázquez, Mercedes Piferrer, Francesc
author_facet	Blázquez, Mercedes Piferrer, Francesc
author_sort	Blázquez, Mercedes
title	Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)
title_short	Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)
title_full	Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)
title_fullStr	Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)
title_full_unstemmed	Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)
title_sort	cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of p450 aromatase in juvenile sea bass (dicentrarchus labrax)
publisher	Elsevier
publishDate	2004-02-26
url	http://hdl.handle.net/10261/16828
work_keys_str_mv	AT blazquezmercedes cloningsequenceanalysistissuedistributionandsexspecificexpressionoftheneuralformofp450aromataseinjuvenileseabassdicentrarchuslabrax AT piferrerfrancesc cloningsequenceanalysistissuedistributionandsexspecificexpressionoftheneuralformofp450aromataseinjuvenileseabassdicentrarchuslabrax
_version_	1777665688743182336

Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax)

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