Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass

Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass

Show other versions (1)

Epigenetics and Periconception Environment : Proceedings of the EPICONCEPT Conference 2015, 6-7 October 2015, Hersonissos, Crete, Greece.-- 1 page

Saved in:
Bibliographic Details
Main Authors: Anastasiadi, Dafni, Díaz, Noelia, Piferrer, Francesc
Other Authors: Ministerio de Economía y Competitividad (España)
Format: comunicación de congreso biblioteca
Published: 2015-10-07
Online Access:http://hdl.handle.net/10261/141525
http://dx.doi.org/10.13039/501100003329
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-icm-es-10261-141525
record_format koha
spelling dig-icm-es-10261-1415252022-09-21T09:04:42Z Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass Anastasiadi, Dafni Díaz, Noelia Piferrer, Francesc Ministerio de Economía y Competitividad (España) Epigenetics and Periconception Environment : Proceedings of the EPICONCEPT Conference 2015, 6-7 October 2015, Hersonissos, Crete, Greece.-- 1 page The environmental information received during early development may be integrated into the genome via epigenetic mechanisms and may have short- or long-term consequences on the phenotype. Fish larvae are susceptible to environmental influences due to direct exposure to the water environment. One-year-old European sea bass (Dicentrarchus labrax) exposed to high temperature (HT) during the early development had higher levels of DNA methylation in the promoter of gonadal aromatase (Navarro-Martín et al. 2011. PLoS Genetics 7(12): e1002447). In this study, we focused on the effects of temperature on global DNA methylation and expression of key genes during the early life of sea bass. We considered two periods of temperature treatment: (1) from 0 to 15 days post fertilization (dpf) and (2) from 20 to 60 dpf, at the beginning and end of the thermosensitive period, respectively. In (1), we conducted a dose (temperature)-response experiment and a time-series of switches between low (LT) and HT. We used MSAP to detect global changes in DNA methylation. We found that there were statistically significant differences in global DNA methylation between groups subjected to increasing temperature during the first 15 days of life, while in contrast no statistically significant differences were detected after the second period of treatment. Furthermore, there was, in general, an upregulation of gene expression, with a mean fold change of 12.216 times in the HT group. The expression of enzymes with a key role in DNA methylation establishment and maintenance, dnmt1 and dnmt3, was found significantly higher in the HT group during the first period when compared to the second one. These experiments illustrate the importance of the environmental conditions during the first 15 dpf of sea bass in evoking changes on DNA methylation and gene expression which will probably have enduring phenotypic consequences Supported by MINECO grant AGL2013-41047-R “Epifarm” to FP Peer Reviewed 2016-12-16T11:22:34Z 2016-12-16T11:22:34Z 2015-10-07 2016-12-16T11:22:35Z comunicación de congreso http://purl.org/coar/resource_type/c_5794 isbn: 978-960-6806-16-2 Epigenetics and Periconception Environment : Proceedings of the EPICONCEPT Conference 2015: 13 (2015) http://hdl.handle.net/10261/141525 http://dx.doi.org/10.13039/501100003329 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-41047-R Sí none
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
description Epigenetics and Periconception Environment : Proceedings of the EPICONCEPT Conference 2015, 6-7 October 2015, Hersonissos, Crete, Greece.-- 1 page
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Anastasiadi, Dafni
Díaz, Noelia
Piferrer, Francesc
format comunicación de congreso
author Anastasiadi, Dafni
Díaz, Noelia
Piferrer, Francesc
spellingShingle Anastasiadi, Dafni
Díaz, Noelia
Piferrer, Francesc
Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass
author_sort Anastasiadi, Dafni
title Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass
title_short Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass
title_full Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass
title_fullStr Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass
title_full_unstemmed Temperature effects on global DNA methylation and gene expression during larval development in the European sea bass
title_sort temperature effects on global dna methylation and gene expression during larval development in the european sea bass
publishDate 2015-10-07
url http://hdl.handle.net/10261/141525
http://dx.doi.org/10.13039/501100003329
work_keys_str_mv AT anastasiadidafni temperatureeffectsonglobaldnamethylationandgeneexpressionduringlarvaldevelopmentintheeuropeanseabass
AT diaznoelia temperatureeffectsonglobaldnamethylationandgeneexpressionduringlarvaldevelopmentintheeuropeanseabass
AT piferrerfrancesc temperatureeffectsonglobaldnamethylationandgeneexpressionduringlarvaldevelopmentintheeuropeanseabass
_version_ 1777666520227250176

Similar Items