Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass

Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass

V Jornada de Cromatina i Epigenètica organitzada per la Secció de Biologia Molecular de la Societat Catalana de Biologia (SCB) amb el Barcelona Chromatin Club (BCC) : V Annual Chromatin and Epigenetics Symposium organized by the Molecular Biology section of the Catalan Society of Biology (SCB) and the Barcelona Chromatin Club (BCC), 10 de març de 2015, Barcelona.-- 1 page

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Main Authors: Anastasiadi, Dafni, Piferrer, Francesc
Other Authors: Ministerio de Economía y Competitividad (España)
Format: póster de congreso biblioteca
Published: Societat Catalana de Biologia 2015-03-10
Online Access:http://hdl.handle.net/10261/141233
http://dx.doi.org/10.13039/501100003329
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spelling dig-icm-es-10261-1412332018-06-05T12:19:31Z Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass Anastasiadi, Dafni Piferrer, Francesc Ministerio de Economía y Competitividad (España) V Jornada de Cromatina i Epigenètica organitzada per la Secció de Biologia Molecular de la Societat Catalana de Biologia (SCB) amb el Barcelona Chromatin Club (BCC) : V Annual Chromatin and Epigenetics Symposium organized by the Molecular Biology section of the Catalan Society of Biology (SCB) and the Barcelona Chromatin Club (BCC), 10 de març de 2015, Barcelona.-- 1 page The environment an organism experiences during early development may have a long lasting impact mediated by epigenetic mechanisms. From one hand, temperature is an environmental factor shown to be related with changes in DNA methylation levels and lasting alterations of gene expression. From the ot her hand, farming conditions result in changes in the gene expression level in fish. Here, we surveyed genomewide DNA methylation changes in adult European sea bass (Dicentrarchus labrax) related to temperature and/or farming conditions using next generation sequencing (NGS) technology. In order to study temperature effects, 3-year-old fish raised at low (17o C) or at high (21o C) temperature during the thermosensitive period were used and for the effects of farming fish sampled in the wild were compared to farmed fish raised at low temperature. We employed Reduced Representation Bisulfite Sequencing (RRBS) to measure DNA methylation levels, where, in brief, after digestion with a methylation-sensitive restriction enzyme MspI and bisulfite conversion, libraries are constructed and sequenced by Illumina HiSeq2000. Bioinformatic analysis was adapted to a non-model species genome and performed using R and RRBS-specific packages. Temperature was found to affect ~3500 cytosines in testis and ~12000 cytosines in muscle. Farming conditions affected more CpG sites than only temperature, with testis presenting ~4000 differentially methylated CpGs (DMC) and muscle presenting ~15000 DMCs. The majority of the DMCs in both comparisons were located close to genes and their regulatory elements, as well as in a distance of up to 5000 bp upstream the transcription start site (TSS). In this study, we have shown that the environmental conditions during the early development of sea bass produce changes on DNA methylation that are detected later in life. We are currently using RNA-seq to determine possible alterations in gene expression associated with these changes in DNA methylation Supported by MINECO grant AGL2013-41047-R >Epifarm> to FP Peer Reviewed 2016-12-09T09:15:44Z 2016-12-09T09:15:44Z 2015-03-10 2016-12-09T09:15:44Z póster de congreso http://purl.org/coar/resource_type/c_6670 V Jornada de Cromatina i Epigenètica. Program: 15 (2015) http://hdl.handle.net/10261/141233 http://dx.doi.org/10.13039/501100003329 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-41047-R http://scb.iec.cat/v-jornada-de-cromatina-i-epigenetica/ Sí none Societat Catalana de Biologia
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
description V Jornada de Cromatina i Epigenètica organitzada per la Secció de Biologia Molecular de la Societat Catalana de Biologia (SCB) amb el Barcelona Chromatin Club (BCC) : V Annual Chromatin and Epigenetics Symposium organized by the Molecular Biology section of the Catalan Society of Biology (SCB) and the Barcelona Chromatin Club (BCC), 10 de març de 2015, Barcelona.-- 1 page
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Anastasiadi, Dafni
Piferrer, Francesc
format póster de congreso
author Anastasiadi, Dafni
Piferrer, Francesc
spellingShingle Anastasiadi, Dafni
Piferrer, Francesc
Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass
author_sort Anastasiadi, Dafni
title Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass
title_short Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass
title_full Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass
title_fullStr Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass
title_full_unstemmed Genome-Wide DNA Methylation Changes due to Temperature and Farming Conditions in the European Sea Bass
title_sort genome-wide dna methylation changes due to temperature and farming conditions in the european sea bass
publisher Societat Catalana de Biologia
publishDate 2015-03-10
url http://hdl.handle.net/10261/141233
http://dx.doi.org/10.13039/501100003329
work_keys_str_mv AT anastasiadidafni genomewidednamethylationchangesduetotemperatureandfarmingconditionsintheeuropeanseabass
AT piferrerfrancesc genomewidednamethylationchangesduetotemperatureandfarmingconditionsintheeuropeanseabass
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