Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects

Van Ekert, Evelien; Chauvigné, François; Finn, Roderick N.; Mathew, Lolita G.; Hull, J. Joe; Cerdà, Joan; Fabrick, Jeffrey A.; Research Council of Norway

Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects

13 pages, 5 figures, 3 tables, supplementary data http://dx.doi.org/10.1016/j.ibmb.2016.07.010

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Bibliographic Details
Main Authors:	Van Ekert, Evelien, Chauvigné, François, Finn, Roderick N., Mathew, Lolita G., Hull, J. Joe, Cerdà, Joan, Fabrick, Jeffrey A.
Other Authors:	Research Council of Norway
Format:	artículo biblioteca
Published:	Pergamon Press 2016-10
Subjects:	Bemisia tabaci, Whitefly, Osmoregulation, Aquaporins, Entomoglyceroporin, Major intrinsic protein family,
Online Access:	http://hdl.handle.net/10261/139569 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100000199
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id	dig-icm-es-10261-139569
record_format	koha
spelling	dig-icm-es-10261-1395692019-03-26T10:18:40Z Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects Van Ekert, Evelien Chauvigné, François Finn, Roderick N. Mathew, Lolita G. Hull, J. Joe Cerdà, Joan Fabrick, Jeffrey A. Research Council of Norway Ministerio de Economía y Competitividad (España) Department of Agriculture (US) Bemisia tabaci Whitefly Osmoregulation Aquaporins Entomoglyceroporin Major intrinsic protein family 13 pages, 5 figures, 3 tables, supplementary data http://dx.doi.org/10.1016/j.ibmb.2016.07.010 The Middle East-Asia Minor 1 (MEAM1) whitefly, Bemisia tabaci (Gennadius) is an economically important pest of food, fiber, and ornamental crops. This pest has evolved a number of adaptations to overcome physiological challenges, including 1) the ability to regulate osmotic stress between gut lumen and hemolymph after imbibing large quantities of a low nitrogen, sugar-rich liquid diet; 2) the ability to avoid or prevent dehydration and desiccation, particularly during egg hatching and molting; and 3) to be adapted for survival at elevated temperatures. One superfamily of proteins involved in the maintenance of fluid homeostasis in many organisms includes the aquaporins, which are integral membrane channel proteins that aid in the rapid flux of water and other small solutes across biological membranes. Here, we show that B. tabaci has eight aquaporins (BtAqps), of which seven belong to the classical aquaporin 4-related grade of channels, including Bib, Drip, Prip, and Eglps and one that belongs to the unorthodox grade of aquaporin 12-like channels. B. tabaci has further expanded its repertoire of water channels through the expression of three BtDrip2 amino-terminal splice variants, while other hemipteran species express amino- or carboxyl-terminal isoforms of Drip, Prip, and Eglps. Each BtAqp has unique transcript expression profiles, cellular localization, and/or substrate preference. Our phylogenetic and functional data reveal that hemipteran insects lost the classical glp genes, but have compensated for this by duplicating the eglp genes early in their evolution to comprise at least three separate clades of glycerol transporters This work was supported by base CRIS funding to USDA-ARS, National Program 304 - Crop Protection & Quarantine [Project #2020-22620-022-00D] to J.F., and partially funded by the Research Council of Norway Grants 204813/F20 and 254872/E40 to R.N.F. and the Spanish Ministry of Economy and Competitiveness (MINECO), project AGL2013-41196-R to J.C. E.V.E. was supported by the USDA-ARS Research Associate Program to J.F. Peer Reviewed 2016-10-28T10:16:17Z 2016-10-28T10:16:17Z 2016-10 2016-10-28T10:16:17Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.ibmb.2016.07.010 issn: 0965-1748 e-issn: 1879-0240 Insect Biochemistry and Molecular Biology 77: 39-51 (2016) http://hdl.handle.net/10261/139569 10.1016/j.ibmb.2016.07.010 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100000199 http://dx.doi.org/10.1016/j.ibmb.2016.07.010 Sí none Pergamon Press
institution	ICM ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-icm-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del ICM España
topic	Bemisia tabaci Whitefly Osmoregulation Aquaporins Entomoglyceroporin Major intrinsic protein family Bemisia tabaci Whitefly Osmoregulation Aquaporins Entomoglyceroporin Major intrinsic protein family
spellingShingle	Bemisia tabaci Whitefly Osmoregulation Aquaporins Entomoglyceroporin Major intrinsic protein family Bemisia tabaci Whitefly Osmoregulation Aquaporins Entomoglyceroporin Major intrinsic protein family Van Ekert, Evelien Chauvigné, François Finn, Roderick N. Mathew, Lolita G. Hull, J. Joe Cerdà, Joan Fabrick, Jeffrey A. Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects
description	13 pages, 5 figures, 3 tables, supplementary data http://dx.doi.org/10.1016/j.ibmb.2016.07.010
author2	Research Council of Norway
author_facet	Research Council of Norway Van Ekert, Evelien Chauvigné, François Finn, Roderick N. Mathew, Lolita G. Hull, J. Joe Cerdà, Joan Fabrick, Jeffrey A.
format	artículo
topic_facet	Bemisia tabaci Whitefly Osmoregulation Aquaporins Entomoglyceroporin Major intrinsic protein family
author	Van Ekert, Evelien Chauvigné, François Finn, Roderick N. Mathew, Lolita G. Hull, J. Joe Cerdà, Joan Fabrick, Jeffrey A.
author_sort	Van Ekert, Evelien
title	Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects
title_short	Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects
title_full	Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects
title_fullStr	Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects
title_full_unstemmed	Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects
title_sort	molecular and functional characterization of bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects
publisher	Pergamon Press
publishDate	2016-10
url	http://hdl.handle.net/10261/139569 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100000199
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Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects

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