Monitoring of Alexandrium species in the Mediterranean Sea using a combined filter system-PCR assay detection method
Special issue Harmful Algae 2004.-- 3 pages, 1 figure, 1 table
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| Format: | artículo biblioteca |
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National Inquiry Services Centre (South Africa)
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| Subjects: | ITS-5.8S rDNA, Alexandrium species, PCR, Mediterranean Sea, HAB species, Alexandrium, ITS, HAB, |
| Online Access: | http://hdl.handle.net/10261/121451 |
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dig-icm-es-10261-1214512021-06-09T07:22:25Z Monitoring of Alexandrium species in the Mediterranean Sea using a combined filter system-PCR assay detection method Penna, Antonella Fusco, G. Bertozzini, Elena Giacobbe, Maria Grazia Vila, Magda Galluzzi, Luca Garcés, Esther Lugliè, Antonella Masó, Mercedes Magnani, Mauro ITS-5.8S rDNA Alexandrium species PCR Mediterranean Sea HAB species Alexandrium ITS HAB Special issue Harmful Algae 2004.-- 3 pages, 1 figure, 1 table A filter system for collecting phytoplankton cells coupled to a target species-specific polymerase chain reaction (PCR) assay was performed on a spatial and temporal series of net and seawater samples as part of the Mediterranean Sea EU project Strategy (EVK-CT-2001-00046). The application of PCR allowed rapid detection of several harmful dinoflagellate species and genera, including Alexandrium spp. In this study, oligonucleotide primers targeting the 5.8S rDNA and ITS regions of the genus Alexandrium and the species A. minutum were developed. Field samples were concentrated onto filter membranes, total DNA was extracted from mixed phytoplankton populations and PCR assays were carried out with specific primers. Qualitative PCR results were compared with light and epifluorescence microscopic examinations. Results indicated that the molecular assay was able to detect harmful target cells at concentrations undetectable by microscopy. Application of this filter PCR assay to seawater samples revealed it to be a sensitive and rapid procedure for routine monitoring of field samples. Copyright © NISC Pty Ltd. This study was supported by the EU Strategy Project-EVK3-CT-2001-00046 Peer Reviewed 2006 2015-08-27T07:08:29Z artículo http://purl.org/coar/resource_type/c_6501 issn: 1814-232X e-issn: 1814-2338 African Journal of Marine Science 28(2): 241-243 (2006) http://hdl.handle.net/10261/121451 10.2989/18142320609504155 https://doi.org/10.2989/18142320609504155 none National Inquiry Services Centre (South Africa) Taylor & Francis |
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ITS-5.8S rDNA Alexandrium species PCR Mediterranean Sea HAB species Alexandrium ITS HAB ITS-5.8S rDNA Alexandrium species PCR Mediterranean Sea HAB species Alexandrium ITS HAB |
| spellingShingle |
ITS-5.8S rDNA Alexandrium species PCR Mediterranean Sea HAB species Alexandrium ITS HAB ITS-5.8S rDNA Alexandrium species PCR Mediterranean Sea HAB species Alexandrium ITS HAB Penna, Antonella Fusco, G. Bertozzini, Elena Giacobbe, Maria Grazia Vila, Magda Galluzzi, Luca Garcés, Esther Lugliè, Antonella Masó, Mercedes Magnani, Mauro Monitoring of Alexandrium species in the Mediterranean Sea using a combined filter system-PCR assay detection method |
| description |
Special issue Harmful Algae 2004.-- 3 pages, 1 figure, 1 table |
| format |
artículo |
| topic_facet |
ITS-5.8S rDNA Alexandrium species PCR Mediterranean Sea HAB species Alexandrium ITS HAB |
| author |
Penna, Antonella Fusco, G. Bertozzini, Elena Giacobbe, Maria Grazia Vila, Magda Galluzzi, Luca Garcés, Esther Lugliè, Antonella Masó, Mercedes Magnani, Mauro |
| author_facet |
Penna, Antonella Fusco, G. Bertozzini, Elena Giacobbe, Maria Grazia Vila, Magda Galluzzi, Luca Garcés, Esther Lugliè, Antonella Masó, Mercedes Magnani, Mauro |
| author_sort |
Penna, Antonella |
| title |
Monitoring of Alexandrium species in the Mediterranean Sea using a combined filter system-PCR assay detection method |
| title_short |
Monitoring of Alexandrium species in the Mediterranean Sea using a combined filter system-PCR assay detection method |
| title_full |
Monitoring of Alexandrium species in the Mediterranean Sea using a combined filter system-PCR assay detection method |
| title_fullStr |
Monitoring of Alexandrium species in the Mediterranean Sea using a combined filter system-PCR assay detection method |
| title_full_unstemmed |
Monitoring of Alexandrium species in the Mediterranean Sea using a combined filter system-PCR assay detection method |
| title_sort |
monitoring of alexandrium species in the mediterranean sea using a combined filter system-pcr assay detection method |
| publisher |
National Inquiry Services Centre (South Africa) |
| url |
http://hdl.handle.net/10261/121451 |
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