Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis

Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis

9 pages, 2 figures, 4 tables, supplementary data associated with this article can be foundin the online version at https://doi.org/10.1016/j.envres.2014.09.024

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Main Authors: Solé, Montserrat, Fortuny, Anna, Mañanós, Evaristo L.
Format: artículo biblioteca
Published: Academic Press 2014-11
Subjects:Ethinylestradiol, Gemfibrozil, Ketoconazole, Nonylphenol, Cytochrome P450,
Online Access:http://hdl.handle.net/10261/108805
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spelling dig-icm-es-10261-1088052020-12-09T17:48:12Z Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis Solé, Montserrat Fortuny, Anna Mañanós, Evaristo L. Ethinylestradiol Gemfibrozil Ketoconazole Nonylphenol Cytochrome P450 9 pages, 2 figures, 4 tables, supplementary data associated with this article can be foundin the online version at https://doi.org/10.1016/j.envres.2014.09.024 In recent years, Solea senegalensis has increasingly been used in pollution monitoring studies. In order to assess its response to some particular widespread pollutants, juveniles of S. senegalensis were administered an intraperitoneal injection of the model aryl hydrocarbon receptor agonist β-naphtoflavone (βNF) and chemicals of environmental concern, such as the fungicide ketoconazole (KETO), the lipid regulator gemfibrozil (GEM), the surfactant nonylphenol (NP) and the synthetic hormone ethinylestradiol (EE2). Two days after injection, the effect of these chemicals was followed up as alterations of hepatic microsomal activities of the cytochrome P450 (CYPs) and associated reductases, carboxylesterases (CbEs) and the conjugation enzyme uridine diphosphate glucuronyltransferase (UDPGT). In the cytosolic fraction of the liver, the effect on CbEs, glutathione S-transferase (GST) and antioxidant activities was also considered. Alterations on the endocrine reproductive system were evaluated by plasma levels of vitellogenin (VTG) and the sex steroids estradiol (E2), testosterone (T), 11-ketotestosterone (11KT) and the progestin 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P). Injection with the model compound βNF induced the hydrolysis rate of the seven CYP substrates assayed. The xenobiotic GEM induced three CYP-related activities (e.g. ECOD) and UDPGT, but depressed antioxidant defenses. EE2 induced four CYPs, more significantly ECOD and BFCOD activities. The xenoestrogens NP and EE2 altered the activities of CbE in microsomes and catalase, and were the only treatments that induced de novo VTG synthesis. In addition, the progestin 17,20β-P, was induced in NP-injected fish. None of the treatments caused statistically significant effects on steroid plasma levels. In conclusion, the CYP substrates assayed responded specifically to treatments and juveniles of S. senegalensis appear good candidates for assessing xenobiotics exposure. © 2014 Elsevier Inc. This work was financed by the Ministry of Science and Innovation of Spain (ref CTM2010-16611) Peer Reviewed 2014-12-17T07:32:50Z 2014-12-17T07:32:50Z 2014-11 2014-12-17T07:32:50Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.envres.2014.09.024 issn: 0013-9351 e-issn: 1096-0953 Environmental Research 135: 227-235 (2014) http://hdl.handle.net/10261/108805 10.1016/j.envres.2014.09.024 https://doi.org/10.1016/j.envres.2014.09.024 none Academic Press
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
topic Ethinylestradiol
Gemfibrozil
Ketoconazole
Nonylphenol
Cytochrome P450
Ethinylestradiol
Gemfibrozil
Ketoconazole
Nonylphenol
Cytochrome P450
spellingShingle Ethinylestradiol
Gemfibrozil
Ketoconazole
Nonylphenol
Cytochrome P450
Ethinylestradiol
Gemfibrozil
Ketoconazole
Nonylphenol
Cytochrome P450
Solé, Montserrat
Fortuny, Anna
Mañanós, Evaristo L.
Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis
description 9 pages, 2 figures, 4 tables, supplementary data associated with this article can be foundin the online version at https://doi.org/10.1016/j.envres.2014.09.024
format artículo
topic_facet Ethinylestradiol
Gemfibrozil
Ketoconazole
Nonylphenol
Cytochrome P450
author Solé, Montserrat
Fortuny, Anna
Mañanós, Evaristo L.
author_facet Solé, Montserrat
Fortuny, Anna
Mañanós, Evaristo L.
author_sort Solé, Montserrat
title Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis
title_short Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis
title_full Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis
title_fullStr Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis
title_full_unstemmed Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis
title_sort effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of solea senegalensis
publisher Academic Press
publishDate 2014-11
url http://hdl.handle.net/10261/108805
work_keys_str_mv AT solemontserrat effectsofselectedxenobioticsonhepaticandplasmaticbiomarkersinjuvenilesofsoleasenegalensis
AT fortunyanna effectsofselectedxenobioticsonhepaticandplasmaticbiomarkersinjuvenilesofsoleasenegalensis
AT mananosevaristol effectsofselectedxenobioticsonhepaticandplasmaticbiomarkersinjuvenilesofsoleasenegalensis
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