Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: a functional role for gene repression
15 páginas, 8 figuras y 3 tablas estadísticas
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
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John Wiley & Sons
2013
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| Subjects: | Arabidopsis, Gene repression, Giant cell, Meloidogyne, Peroxidase, Tomato, Transcriptome., |
| Online Access: | http://hdl.handle.net/10261/82659 |
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dig-ica-es-10261-826592023-02-03T09:49:16Z Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: a functional role for gene repression Portillo, Mary Cabrera, Javier Lindsey, Keith Topping, Jen Andrés, Mª Fé Emiliozzi, Mariana Oliveros, Juan C. García-Casado, G. Solano, Roberto Koltai, Hinanit Resnick, Nathalie Fenoll, Carmen Escobar, Carolina Arabidopsis Gene repression Giant cell Meloidogyne Peroxidase Tomato Transcriptome. 15 páginas, 8 figuras y 3 tablas estadísticas Root-knot nematodes (RKNs) induce giant cells (GCs) from root vascular cells inside the galls. Accompanying molecular changes as a function of infection time and across different species, and their functional impact, are still poorly understood. Thus, the transcriptomes of tomato galls and laser capture microdissected (LCM) GCs over the course of parasitism were compared with those of Arabidopsis, and functional analysis of a repressed gene was performed. Microarray hybridization with RNA from galls and LCM GCs, infection–reproduction tests and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) transcriptional profiles in susceptible and resistant (Mi-1) lines were performed in tomato. Tomato GC-induced genes include some possibly contributing to the epigenetic control of GC identity. GC-repressed genes are conserved between tomato and Arabidopsis, notably those involved in lignin deposition. However, genes related to the regulation of gene expression diverge, suggesting that diverse transcriptional regulators mediate common responses leading to GC formation in different plant species. TPX1, a cell wall peroxidase specifically involved in lignification, was strongly repressed in GCs/galls, but induced in a nearly isogenic Mi-1 resistant line on nematode infection. TPX1 overexpression in susceptible plants hindered nematode reproduction and GC expansion. Time-course and cross-species comparisons of gall and GC transcriptomes provide novel insights pointing to the relevance of gene repression during RKN establishment. Spanish Government to C.E. (AGL2010- 17388) and C.F. (CSD2007-057), and the Castilla-La Mancha Government to C.F. and C.E. (PCI08-0074-0294). Peer reviewed 2013-09-24T11:18:34Z 2013-09-24T11:18:34Z 2013 artículo http://purl.org/coar/resource_type/c_6501 New Phytologist 197: 1276–1290 (2013) 0028-646X http://hdl.handle.net/10261/82659 10.1111/nph.12121 1469-8137 en http://dx.doi.org/ 10.1111/nph.12121 none John Wiley & Sons |
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España |
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ES |
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dig-ica-es |
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biblioteca |
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Europa del Sur |
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Biblioteca del ICA España |
| language |
English |
| topic |
Arabidopsis Gene repression Giant cell Meloidogyne Peroxidase Tomato Transcriptome. Arabidopsis Gene repression Giant cell Meloidogyne Peroxidase Tomato Transcriptome. |
| spellingShingle |
Arabidopsis Gene repression Giant cell Meloidogyne Peroxidase Tomato Transcriptome. Arabidopsis Gene repression Giant cell Meloidogyne Peroxidase Tomato Transcriptome. Portillo, Mary Cabrera, Javier Lindsey, Keith Topping, Jen Andrés, Mª Fé Emiliozzi, Mariana Oliveros, Juan C. García-Casado, G. Solano, Roberto Koltai, Hinanit Resnick, Nathalie Fenoll, Carmen Escobar, Carolina Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: a functional role for gene repression |
| description |
15 páginas, 8 figuras y 3 tablas estadísticas |
| format |
artículo |
| topic_facet |
Arabidopsis Gene repression Giant cell Meloidogyne Peroxidase Tomato Transcriptome. |
| author |
Portillo, Mary Cabrera, Javier Lindsey, Keith Topping, Jen Andrés, Mª Fé Emiliozzi, Mariana Oliveros, Juan C. García-Casado, G. Solano, Roberto Koltai, Hinanit Resnick, Nathalie Fenoll, Carmen Escobar, Carolina |
| author_facet |
Portillo, Mary Cabrera, Javier Lindsey, Keith Topping, Jen Andrés, Mª Fé Emiliozzi, Mariana Oliveros, Juan C. García-Casado, G. Solano, Roberto Koltai, Hinanit Resnick, Nathalie Fenoll, Carmen Escobar, Carolina |
| author_sort |
Portillo, Mary |
| title |
Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: a functional role for gene repression |
| title_short |
Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: a functional role for gene repression |
| title_full |
Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: a functional role for gene repression |
| title_fullStr |
Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: a functional role for gene repression |
| title_full_unstemmed |
Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: a functional role for gene repression |
| title_sort |
distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with arabidopsis: a functional role for gene repression |
| publisher |
John Wiley & Sons |
| publishDate |
2013 |
| url |
http://hdl.handle.net/10261/82659 |
| work_keys_str_mv |
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