New structure in cell puncture activities by aphid stylets: a dual-mode EPG study

New structure in cell puncture activities by aphid stylets: a dual-mode EPG study

15páginas, figuras y tablas estadísticas.

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Bibliographic Details
Main Authors: Tjallingii, W. F., Garzo, E., Fereres, Alberto
Format: artículo biblioteca
Language:English
Published: Wiley-Blackwell 2010
Subjects:Full EPG, R-EPG, Aphi gossypii, Cucumovirus, Cucumber mosaic virus, CMV, Non-persistent transmission, Cucumis melo, melon, Brevicoryne brassicae, Myzus persicae, Hemiptera, Aphididae,
Online Access:http://hdl.handle.net/10261/37685
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spelling dig-ica-es-10261-376852020-05-28T11:24:46Z New structure in cell puncture activities by aphid stylets: a dual-mode EPG study Tjallingii, W. F. Garzo, E. Fereres, Alberto Full EPG R-EPG Aphi gossypii Cucumovirus Cucumber mosaic virus CMV Non-persistent transmission Cucumis melo melon Brevicoryne brassicae Myzus persicae Hemiptera Aphididae 15páginas, figuras y tablas estadísticas. Intracellular punctures by aphid stylets appear as potential drop (pd)waveforms inDCelectrical penetration graph (EPG) recordings. We used a dual-EPG device that recorded in one channel the ‘full EPG’ with R-plus emf-components (i.e., the usual DC EPG) and concurrently in a second channel the ‘R-EPG’ with R-components only. The circuit of the latter channel was an optimised amplitude modulation (AM) version derived from early (before 1990) AC systems. We also made some ‘emf- EPG’ recordings using a separate high input resistance ‘emf-amplifier’ sensitive to emf-components only. The intracellular pd waveforms have previously been divided into three subphases, and we aimed to distinguish and separate these subphases more accurately by the dual-EPG recordings than with the normal full EPG only. In this study, we temporarily distinguished five subphases (a–e), but unequivocal distinction of only a few of these appeared possible, in spite of the information coming from the two signals. The lack of clearly separable features in R-EPG signals often provided serious difficulties in pd recognition without the concurrent full EPG, but once located, only subphase II-2 features were clear and supported the II-2 data from the full EPG. Consequently, we could not distinguish subphases of complete pd waveforms better with additional R-EPG information during cell punctures by Aphis gossypii Glover (Hemiptera: Aphididae). In Brevicoryne brassicae (L.) (Hemiptera: Aphididae), however, distinguishing II-2 subphases in the full EPG was sometimes a problem. Our detailed dual-EPG observations showed some waveform continuity from halfway into the II-1 subphase (start of the newly recognised subphase b) until the end of the pd, with a strong but variable emf origin. This waveformtended to overrule other subphase waveforms in B. brassicae more than in A. gossypii and Myzus persicae (Sulzer) (Hemiptera: Aphididae). Subphase waveforms in full EPGs were especially difficult to recognise when pd periods had been interrupted in a virus inoculation experiment and additional R-EPG information could then be useful. This inoculation experiment showed again that only the first subphase (II-1) contributes to virus (Cucumbermosaic virus) inoculation by A. gossypii. In B. brassicae, the benefit of concurrent R-EPG information in such virus experiments is presently under further investigation. Apart from this special application to virus experiments, we do not recommend the routine use of the dual-EPG device. Furthermore, we do not advocate the distinction of more than the previously recognised three intracellular pd subphases as a feasible option in future studies. Analysis of EPGs with concurrent R-EPGs requires substantially more analysis work without yielding consistently useful additional insights. This confirms earlier dual-EPG results from thrips. project has been funded by the Spanish government, Ministry of Education and Science grants SAB2005-0009 and AGL-2003-07532-C03-01. We also thank Maria Plaza for technical assistance and Glen Powell for critical comments and language corrections. Peer reviewed 2011-07-12T09:58:24Z 2011-07-12T09:58:24Z 2010 artículo http://purl.org/coar/resource_type/c_6501 Entomologia Experimentalis et Applicata 135: 193-207 (2010) 0013-8703 http://hdl.handle.net/10261/37685 10.1111/j.1570-7458.2010.00983.x en http://doi.org/10.1111/j.1570-7458.2010.00983.x none Wiley-Blackwell
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collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ica-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICA España
language English
topic Full EPG
R-EPG
Aphi gossypii
Cucumovirus
Cucumber mosaic virus
CMV
Non-persistent transmission
Cucumis melo
melon
Brevicoryne brassicae
Myzus persicae
Hemiptera
Aphididae
Full EPG
R-EPG
Aphi gossypii
Cucumovirus
Cucumber mosaic virus
CMV
Non-persistent transmission
Cucumis melo
melon
Brevicoryne brassicae
Myzus persicae
Hemiptera
Aphididae
spellingShingle Full EPG
R-EPG
Aphi gossypii
Cucumovirus
Cucumber mosaic virus
CMV
Non-persistent transmission
Cucumis melo
melon
Brevicoryne brassicae
Myzus persicae
Hemiptera
Aphididae
Full EPG
R-EPG
Aphi gossypii
Cucumovirus
Cucumber mosaic virus
CMV
Non-persistent transmission
Cucumis melo
melon
Brevicoryne brassicae
Myzus persicae
Hemiptera
Aphididae
Tjallingii, W. F.
Garzo, E.
Fereres, Alberto
New structure in cell puncture activities by aphid stylets: a dual-mode EPG study
description 15páginas, figuras y tablas estadísticas.
format artículo
topic_facet Full EPG
R-EPG
Aphi gossypii
Cucumovirus
Cucumber mosaic virus
CMV
Non-persistent transmission
Cucumis melo
melon
Brevicoryne brassicae
Myzus persicae
Hemiptera
Aphididae
author Tjallingii, W. F.
Garzo, E.
Fereres, Alberto
author_facet Tjallingii, W. F.
Garzo, E.
Fereres, Alberto
author_sort Tjallingii, W. F.
title New structure in cell puncture activities by aphid stylets: a dual-mode EPG study
title_short New structure in cell puncture activities by aphid stylets: a dual-mode EPG study
title_full New structure in cell puncture activities by aphid stylets: a dual-mode EPG study
title_fullStr New structure in cell puncture activities by aphid stylets: a dual-mode EPG study
title_full_unstemmed New structure in cell puncture activities by aphid stylets: a dual-mode EPG study
title_sort new structure in cell puncture activities by aphid stylets: a dual-mode epg study
publisher Wiley-Blackwell
publishDate 2010
url http://hdl.handle.net/10261/37685
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AT garzoe newstructureincellpunctureactivitiesbyaphidstyletsadualmodeepgstudy
AT fereresalberto newstructureincellpunctureactivitiesbyaphidstyletsadualmodeepgstudy
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