Hsp90 Gene Is Required for Mi-1-Mediated Resistance of Tomato to the Whitefly Bemisia tabaci
17 Pág.
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| Language: | English |
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Multidisciplinary Digital Publishing Institute
2023-02-01
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| Subjects: | Bemisia tabaci, HSP90, Mi-1, VIGS, Insects, Pest resistance, Tomato, Whiteflies, http://metadata.un.org/sdg/14, http://metadata.un.org/sdg/15, |
| Online Access: | http://hdl.handle.net/10261/309732 https://api.elsevier.com/content/abstract/scopus_id/85147887728 |
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dig-ica-es-10261-3097322024-05-18T20:35:30Z Hsp90 Gene Is Required for Mi-1-Mediated Resistance of Tomato to the Whitefly Bemisia tabaci Pascual, Susana Rodríguez-Álvarez, Clara I. Kaloshian, Isgouhi Nombela, Gloria Ministerio de Educación y Ciencia (España) Pascual, Susana [0000-0001-9317-0938] Kaloshian, Isgouhi [0000-0002-9873-2810] Nombela, Gloria [0000-0001-7976-6487] Bemisia tabaci HSP90 Mi-1 VIGS Insects Pest resistance Tomato Whiteflies http://metadata.un.org/sdg/14 http://metadata.un.org/sdg/15 17 Pág. The Mi-1 gene of tomato (Solanum lycopersicum) confers resistance against some nematodes and insects, but the resistance mechanisms differ depending on the harmful organism, as a hypersensitive reaction (HR) occurs only in the case of nematodes. The gene Rme1 is required for Mi-1-mediated resistance to nematodes, aphids, and whiteflies, and several additional proteins also play a role in this resistance. Among them, the involvement of the chaperone HSP90 has been demonstrated in Mi-1-mediated resistance for aphids and nematodes, but not for whiteflies. In this work, we studied the implication of the Hsp90 gene in the Mi-1 resistance against the whitefly Bemisia tabaci by means of Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS). The silencing of the Hsp90 gene in tomato Motelle plants carrying the Mi-1 gene resulted in a decrease in resistance to whiteflies, as oviposition values were significantly higher than those on non-silenced plants. This decrease in resistance was equivalent to that caused by the silencing of the Mi-1 gene itself. Infiltration with the control TRV vector did not alter Mi-1 mediated resistance to B. tabaci. Similar to the Mi-1 gene, silencing of Hsp90-1 occurs partially, as silenced plants showed a significant but not complete suppression of gene expression. Thus, our results demonstrate the requirement of Hsp90 in the Mi-1-mediated resistance to B. tabaci and reinforce the hypothesis of a common model for this resistance to nematodes and insects. This research was funded by a Project (AGL2007-65854/AGR) from the Plan Nacional I+D+I, Spanish Ministry of Education and Science. Clara I. Rodríguez Álvarez was financially supported by a fellowship/contract (AP2006-1035) from the Spanish FPU Program. Peer reviewed 2023-05-26T08:09:37Z 2023-05-26T08:09:37Z 2023-02-01 artículo http://purl.org/coar/resource_type/c_6501 Plants 12(3): e641 (2023) http://hdl.handle.net/10261/309732 10.3390/plants12030641 2223-7747 36771723 2-s2.0-85147887728 https://api.elsevier.com/content/abstract/scopus_id/85147887728 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MEC//AGL2007-65854/ES/RESISTENCIA VEGETAL A LAS PLAGAS DEL TOMATE, BEMISIA TABACI Y MELOIDOGYNE SPP.: IDENTIFICACION DE GENES IMPLICADOS Y ANALISIS COMPARATIVO DE LAS RESISTENCIAS CONGENITA E INDUCIDA, DIRIGIDO A PROGRAM/ Departamento de Protección Vegetal Publisher's version https://doi.org/10.3390/plants12030641 Sí open Multidisciplinary Digital Publishing Institute |
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Bemisia tabaci HSP90 Mi-1 VIGS Insects Pest resistance Tomato Whiteflies http://metadata.un.org/sdg/14 http://metadata.un.org/sdg/15 Bemisia tabaci HSP90 Mi-1 VIGS Insects Pest resistance Tomato Whiteflies http://metadata.un.org/sdg/14 http://metadata.un.org/sdg/15 |
| spellingShingle |
Bemisia tabaci HSP90 Mi-1 VIGS Insects Pest resistance Tomato Whiteflies http://metadata.un.org/sdg/14 http://metadata.un.org/sdg/15 Bemisia tabaci HSP90 Mi-1 VIGS Insects Pest resistance Tomato Whiteflies http://metadata.un.org/sdg/14 http://metadata.un.org/sdg/15 Pascual, Susana Rodríguez-Álvarez, Clara I. Kaloshian, Isgouhi Nombela, Gloria Hsp90 Gene Is Required for Mi-1-Mediated Resistance of Tomato to the Whitefly Bemisia tabaci |
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17 Pág. |
| author2 |
Ministerio de Educación y Ciencia (España) |
| author_facet |
Ministerio de Educación y Ciencia (España) Pascual, Susana Rodríguez-Álvarez, Clara I. Kaloshian, Isgouhi Nombela, Gloria |
| format |
artículo |
| topic_facet |
Bemisia tabaci HSP90 Mi-1 VIGS Insects Pest resistance Tomato Whiteflies http://metadata.un.org/sdg/14 http://metadata.un.org/sdg/15 |
| author |
Pascual, Susana Rodríguez-Álvarez, Clara I. Kaloshian, Isgouhi Nombela, Gloria |
| author_sort |
Pascual, Susana |
| title |
Hsp90 Gene Is Required for Mi-1-Mediated Resistance of Tomato to the Whitefly Bemisia tabaci |
| title_short |
Hsp90 Gene Is Required for Mi-1-Mediated Resistance of Tomato to the Whitefly Bemisia tabaci |
| title_full |
Hsp90 Gene Is Required for Mi-1-Mediated Resistance of Tomato to the Whitefly Bemisia tabaci |
| title_fullStr |
Hsp90 Gene Is Required for Mi-1-Mediated Resistance of Tomato to the Whitefly Bemisia tabaci |
| title_full_unstemmed |
Hsp90 Gene Is Required for Mi-1-Mediated Resistance of Tomato to the Whitefly Bemisia tabaci |
| title_sort |
hsp90 gene is required for mi-1-mediated resistance of tomato to the whitefly bemisia tabaci |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2023-02-01 |
| url |
http://hdl.handle.net/10261/309732 https://api.elsevier.com/content/abstract/scopus_id/85147887728 |
| work_keys_str_mv |
AT pascualsusana hsp90geneisrequiredformi1mediatedresistanceoftomatotothewhiteflybemisiatabaci AT rodriguezalvarezclarai hsp90geneisrequiredformi1mediatedresistanceoftomatotothewhiteflybemisiatabaci AT kaloshianisgouhi hsp90geneisrequiredformi1mediatedresistanceoftomatotothewhiteflybemisiatabaci AT nombelagloria hsp90geneisrequiredformi1mediatedresistanceoftomatotothewhiteflybemisiatabaci |
| _version_ |
1802820063544213504 |