Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain

Ramírez-Bahena, Marta-Helena; Cuesta Sánchez, Mª José; Tejedor, Carmen; Igual, José Mariano; Fernández-Pascual, Mercedes; Peix, Álvaro; Junta de Castilla y León

Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain

8 páginas, 2 tablas y 2 figuras. We, (the authors), acknowledge IDEBIO S. L. and Dr P. García-Benavides and Dr J. L. Palomo for their help and support. M.H.R.B. is the recipient of a JAE-Doc researcher contract from CSIC cofinanced by ERDF. We are grateful to V. Souza-Egipsy from ICA Electron Microscopy Service.

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Bibliographic Details
Main Authors:	Ramírez-Bahena, Marta-Helena, Cuesta Sánchez, Mª José, Tejedor, Carmen, Igual, José Mariano, Fernández-Pascual, Mercedes, Peix, Álvaro
Other Authors:	Junta de Castilla y León
Format:	artículo biblioteca
Language:	English
Published:	International Union of Microbiological Societies
Subjects:	BSTT44, Endophytic bacteria, 16S rRNA, Pseudomonas psychrophila, Pseudomonas lundensis, Aesculin hydrolysis,
Online Access:	http://hdl.handle.net/10261/122451 http://dx.doi.org/10.13039/501100014180
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id	dig-ica-es-10261-122451
record_format	koha
spelling	dig-ica-es-10261-1224512021-06-07T07:41:10Z Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain Ramírez-Bahena, Marta-Helena Cuesta Sánchez, Mª José Tejedor, Carmen Igual, José Mariano Fernández-Pascual, Mercedes Peix, Álvaro Junta de Castilla y León BSTT44 Endophytic bacteria 16S rRNA Pseudomonas psychrophila Pseudomonas lundensis Aesculin hydrolysis 8 páginas, 2 tablas y 2 figuras. We, (the authors), acknowledge IDEBIO S. L. and Dr P. García-Benavides and Dr J. L. Palomo for their help and support. M.H.R.B. is the recipient of a JAE-Doc researcher contract from CSIC cofinanced by ERDF. We are grateful to V. Souza-Egipsy from ICA Electron Microscopy Service. A bacterial strain named BSTT44T was isolated in the course of a study of endophytic bacteria occurring in stems and roots of potato growing in a soil from Salamanca, Spain. The 16S rRNA gene sequence had 99.7% identity with respect to that of its closest relative, Pseudomonas psychrophila E-3T, and the next most closely related type strains were those of Pseudomonas fragi, with 99.6% similarity, Pseudomonas deceptionensis, with 99.2% similarity, and Pseudomonas lundensis, with 99.0% similarity; these results indicate that BSTT44T should be classified within the genus Pseudomonas. Analysis of the housekeeping genes rpoB, rpoD and gyrB confirmed its phylogenetic affiliation and showed identities lower than 92% in all cases with respect to the above-mentioned closest relatives. Cells of the strain bore one polar–subpolar flagellum. The respiratory quinone was Q-9.The major fatty acids were C16:0, C18:1v7c and summed feature 3 (C16:1v7c and/or C16:1v6c). The strain was oxidase-, catalase- and urease-positive and the arginine dihydrolase system was present, but tests for nitrate reduction, b-galactosidase production and aesculin hydrolysis were negative. It could grow at 35 8C and at pH 5–9.The DNA G+C content was 60.2 mol%. DNA–DNA hybridization results showed less than 48% relatedness with respect to the type strains of the four most closely related species. Therefore, the combined results of genotypic, phenotypic and chemotaxonomic analyses support the classification of strain BSTT44 into a novel species of the genus Pseudomonas, for which the name Pseudomonas endophytica sp. nov. is proposed. The type strain is BSTT44T (5LMG 28456T5CECT 8691T). Project CSI327A12-2 Peer reviewed 2015-08 artículo http://purl.org/coar/resource_type/c_6501 International Journal of Systematic and Evolutionary Microbiology 65: 2110–2117 (2015) 1466-5026 http://hdl.handle.net/10261/122451 10.1099/ijs.0.000230 http://dx.doi.org/10.13039/501100014180 en Publisher's version http://dx.doi.org/10.1099/ijs.0.000230 Sí open International Union of Microbiological Societies
institution	ICA ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-ica-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del ICA España
language	English
topic	BSTT44 Endophytic bacteria 16S rRNA Pseudomonas psychrophila Pseudomonas lundensis Aesculin hydrolysis BSTT44 Endophytic bacteria 16S rRNA Pseudomonas psychrophila Pseudomonas lundensis Aesculin hydrolysis
spellingShingle	BSTT44 Endophytic bacteria 16S rRNA Pseudomonas psychrophila Pseudomonas lundensis Aesculin hydrolysis BSTT44 Endophytic bacteria 16S rRNA Pseudomonas psychrophila Pseudomonas lundensis Aesculin hydrolysis Ramírez-Bahena, Marta-Helena Cuesta Sánchez, Mª José Tejedor, Carmen Igual, José Mariano Fernández-Pascual, Mercedes Peix, Álvaro Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain
description	8 páginas, 2 tablas y 2 figuras. We, (the authors), acknowledge IDEBIO S. L. and Dr P. García-Benavides and Dr J. L. Palomo for their help and support. M.H.R.B. is the recipient of a JAE-Doc researcher contract from CSIC cofinanced by ERDF. We are grateful to V. Souza-Egipsy from ICA Electron Microscopy Service.
author2	Junta de Castilla y León
author_facet	Junta de Castilla y León Ramírez-Bahena, Marta-Helena Cuesta Sánchez, Mª José Tejedor, Carmen Igual, José Mariano Fernández-Pascual, Mercedes Peix, Álvaro
format	artículo
topic_facet	BSTT44 Endophytic bacteria 16S rRNA Pseudomonas psychrophila Pseudomonas lundensis Aesculin hydrolysis
author	Ramírez-Bahena, Marta-Helena Cuesta Sánchez, Mª José Tejedor, Carmen Igual, José Mariano Fernández-Pascual, Mercedes Peix, Álvaro
author_sort	Ramírez-Bahena, Marta-Helena
title	Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain
title_short	Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain
title_full	Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain
title_fullStr	Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain
title_full_unstemmed	Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain
title_sort	pseudomonas endophytica sp. nov., isolated from stem tissue of solanum tuberosum l. in spain
publisher	International Union of Microbiological Societies
url	http://hdl.handle.net/10261/122451 http://dx.doi.org/10.13039/501100014180
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Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain

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