Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells

Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells

30 páginas, 9 figuras, 1 tabla, 13 páginas de explicaciones suplementarias de las figuras, más otras 10 figuras suplementarias,

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Main Authors: Tejada-Jiménez, Manuel, Castro-Rodríguez, Rosario, Kryvoruchko, Igor, Lucas, M. Mercedes, Udvardi, Michael, Imperial, Juan, González-Guerrero, Manuel
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Language:English
Published: American Society of Plant Biologists 2015-05
Subjects:Medicago truncatula, Nodule Cells, Macrophage Protein, Iron, symbiotic nitrogen fixation (SNF), Apoplast, rhizobia-infected cells,
Online Access:http://hdl.handle.net/10261/121178
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000781
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spelling dig-ica-es-10261-1211782021-12-27T16:20:55Z Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells Tejada-Jiménez, Manuel Castro-Rodríguez, Rosario Kryvoruchko, Igor Lucas, M. Mercedes Udvardi, Michael Imperial, Juan González-Guerrero, Manuel Ministerio de Economía y Competitividad (España) European Research Council Medicago truncatula Nodule Cells Macrophage Protein Iron symbiotic nitrogen fixation (SNF) Apoplast rhizobia-infected cells 30 páginas, 9 figuras, 1 tabla, 13 páginas de explicaciones suplementarias de las figuras, más otras 10 figuras suplementarias, Iron is critical for symbiotic nitrogen fixation (SNF) as a key component of multiple ferroproteins involved in this biological process. In the model legume Medicago truncatula, iron is delivered by the vasculature to the infection/maturation zone (zone II) of the nodule, where it is released to the apoplast. From there, plasma membrane iron transporters move it into rhizobia-containing cells, where iron is used as the cofactor of multiple plant and rhizobial proteins (e.g. plant leghemoglobin and bacterial nitrogenase). MtNramp1 (Medtr3g088460) is the M. truncatula Natural Resistance-Associated Macrophage Protein family member, with the highest expression levels in roots and nodules. Immunolocalization studies indicate that MtNramp1 is mainly targeted to the plasma membrane. A loss-of-function nramp1 mutant exhibited reduced growth compared with the wild type under symbiotic conditions, but not when fertilized with mineral nitrogen. Nitrogenase activity was low in the mutant, whereas exogenous iron and expression of wild-type MtNramp1 in mutant nodules increased nitrogen fixation to normal levels. These data are consistent with a model in which MtNramp1 is the main transporter responsible for apoplastic iron uptake by rhizobia-infected cells in zone II. This work was supported by the Spanish Ministry of Economy and Competitiveness (grant no. AGL–2012–32974 to M.G.-G. and Formación del Personal Investigador fellowship no. BES–2013– 062674 to R.C.-R.), a Marie Curie International Reintegration grant (grant no. IRG–2010–276771 to M.G.-G.), a European Research Council Starting grant (grant no. ERC–2013–StG–335284 to M.G.-G.), and a Ramón y Cajal fellowship (no. RYC–2010–06363 to M.G.-G.). ACKNOWLEDGMENTS We thank Dr. Alonso Rodríguez-Navarro (Universidad Politécnica deMadrid) for providing plasmid pYPGE15, Dr. David Eide (University of Wisconsin, Madison) for the strain fet3/fet4 and its parental strain, Dr. Benoit Lefebvre (Laboratoire des Interactions Plantes Microorganismes) for the pBI101 vector, Dr. Florian Frugier (Institut des Sciences du Vegétal) for the pFRN vector, Dr. Rosario Haro (Universidad Politécnica de Madrid) for the organelle-specific markers, Dr. Regla Bustos (Instituto Nacional de Investigaciones Agroalimentarias) for the agroinfiltration protocol, Dr. Larry Peterson for the Casparian strip visualization method, and Dr. Tsuyoshi Nakagawa (Shiimane University) for the pGWB vectors. Peer reviewed 2015-08-18T11:22:41Z 2015-08-18T11:22:41Z 2015-05 artículo http://purl.org/coar/resource_type/c_6501 Plant Physiology, 168: 258–272 (2015) 0032-0889 http://hdl.handle.net/10261/121178 10.1104/pp.114.254672 1532-2548 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000781 25818701 en Publisher's version www.plantphysiol.org/cgi/doi/10.1104/pp.114.254672 Sí open American Society of Plant Biologists
institution ICA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ica-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICA España
language English
topic Medicago truncatula
Nodule Cells
Macrophage Protein
Iron
symbiotic nitrogen fixation (SNF)
Apoplast
rhizobia-infected cells
Medicago truncatula
Nodule Cells
Macrophage Protein
Iron
symbiotic nitrogen fixation (SNF)
Apoplast
rhizobia-infected cells
spellingShingle Medicago truncatula
Nodule Cells
Macrophage Protein
Iron
symbiotic nitrogen fixation (SNF)
Apoplast
rhizobia-infected cells
Medicago truncatula
Nodule Cells
Macrophage Protein
Iron
symbiotic nitrogen fixation (SNF)
Apoplast
rhizobia-infected cells
Tejada-Jiménez, Manuel
Castro-Rodríguez, Rosario
Kryvoruchko, Igor
Lucas, M. Mercedes
Udvardi, Michael
Imperial, Juan
González-Guerrero, Manuel
Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells
description 30 páginas, 9 figuras, 1 tabla, 13 páginas de explicaciones suplementarias de las figuras, más otras 10 figuras suplementarias,
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Tejada-Jiménez, Manuel
Castro-Rodríguez, Rosario
Kryvoruchko, Igor
Lucas, M. Mercedes
Udvardi, Michael
Imperial, Juan
González-Guerrero, Manuel
format artículo
topic_facet Medicago truncatula
Nodule Cells
Macrophage Protein
Iron
symbiotic nitrogen fixation (SNF)
Apoplast
rhizobia-infected cells
author Tejada-Jiménez, Manuel
Castro-Rodríguez, Rosario
Kryvoruchko, Igor
Lucas, M. Mercedes
Udvardi, Michael
Imperial, Juan
González-Guerrero, Manuel
author_sort Tejada-Jiménez, Manuel
title Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells
title_short Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells
title_full Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells
title_fullStr Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells
title_full_unstemmed Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells
title_sort medicago truncatula natural resistance-associated macrophage protein1 is required for iron uptake by rhizobia-infected nodule cells
publisher American Society of Plant Biologists
publishDate 2015-05
url http://hdl.handle.net/10261/121178
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000781
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