Correlation Between Whitefly (Homoptera: Aleyrodidae) Feeding Behavior and Transmission of Tomato Yellow Leaf Curl Virus
The feeding behavior of the whitefly Bemisia tabaci (Gennadius) was monitored using the electrical penetration graph (EPG) technique during the transmission process of tomato yellow leaf curl geminivirus (TYLCV). The behavior of individual viruliferous whiteflies was recorded on two-leaf stage tomato test plants (Lycopersicon esculentum Mill ‘Riofuego’). A total of 213 whitefly individuals was recorded on single test plants during an inoculation access period that ranged from 3.5 to 14 h. Recordings were classified into 4 categories depending of the waveforms observed: group I, including only waveform C (pathway), was associated with a residual 2.4% TYLCV transmission efficiency; group II, showing stylet pathway and a single E(pd)1 waveform, was associated with a 7.4% transmission efficiency; group III, showing stylet pathway and a single E(pd)1 + E(pd)2 waveform, achieved a 23.4% transmission efficiency; and finally group IV, showing pathway followed by several E(pd)1 + E(pd)2 waveforms, was associated with the highest transmission efficiency (37.5%). A total of 16 different behavioral variables was introduced into a stepwise-backward logistic regression model to determine the variables most related to TYLCV inoculation. Among them, the total duration of E(pd)1 was the most significant variable associated with virus inoculation by B. tabaci (P = 0.002, positive relationship). In addition, the regression analysis indicated a strong positive relationship (P = 0.005) between transmission efficiency and total number of E(pd)1 (t = 4.39, β = 0.45 ± 0.10, r = 0.87), and total duration of waveform E(pd)1 (P = 0.05) (t = 4.00, β = 0.02 ± 0.004, r = 0.94). The minimum phloem contact E(pd)1 threshold period observed for successful inoculation of TYLCV by B. tabaci on tomato plants was 1.8 min. Implications of these results for persistent virus transmission by whiteflies are discussed.
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Entomological Society of America
2000-05
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Subjects: | Bemisia tabaci, Feeding behavior, Tomato yellow leaf curl virus, Transmission, |
Online Access: | http://hdl.handle.net/10261/11467 |
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dig-ica-es-10261-114672009-03-25T23:00:00Z Correlation Between Whitefly (Homoptera: Aleyrodidae) Feeding Behavior and Transmission of Tomato Yellow Leaf Curl Virus Jiang, Y-X. Blas, C. de Barrios, Laura Fereres, Alberto Bemisia tabaci Feeding behavior Tomato yellow leaf curl virus Transmission The feeding behavior of the whitefly Bemisia tabaci (Gennadius) was monitored using the electrical penetration graph (EPG) technique during the transmission process of tomato yellow leaf curl geminivirus (TYLCV). The behavior of individual viruliferous whiteflies was recorded on two-leaf stage tomato test plants (Lycopersicon esculentum Mill ‘Riofuego’). A total of 213 whitefly individuals was recorded on single test plants during an inoculation access period that ranged from 3.5 to 14 h. Recordings were classified into 4 categories depending of the waveforms observed: group I, including only waveform C (pathway), was associated with a residual 2.4% TYLCV transmission efficiency; group II, showing stylet pathway and a single E(pd)1 waveform, was associated with a 7.4% transmission efficiency; group III, showing stylet pathway and a single E(pd)1 + E(pd)2 waveform, achieved a 23.4% transmission efficiency; and finally group IV, showing pathway followed by several E(pd)1 + E(pd)2 waveforms, was associated with the highest transmission efficiency (37.5%). A total of 16 different behavioral variables was introduced into a stepwise-backward logistic regression model to determine the variables most related to TYLCV inoculation. Among them, the total duration of E(pd)1 was the most significant variable associated with virus inoculation by B. tabaci (P = 0.002, positive relationship). In addition, the regression analysis indicated a strong positive relationship (P = 0.005) between transmission efficiency and total number of E(pd)1 (t = 4.39, β = 0.45 ± 0.10, r = 0.87), and total duration of waveform E(pd)1 (P = 0.05) (t = 4.00, β = 0.02 ± 0.004, r = 0.94). The minimum phloem contact E(pd)1 threshold period observed for successful inoculation of TYLCV by B. tabaci on tomato plants was 1.8 min. Implications of these results for persistent virus transmission by whiteflies are discussed. Peer reviewed 2009-03-11T10:48:15Z 2009-03-11T10:48:15Z 2000-05 artículo http://purl.org/coar/resource_type/c_6501 Annals of the Entomological Society of America 93(3):573-579. 2000 0013-8746 http://hdl.handle.net/10261/11467 10.1603/0013-8746(2000)093[0573:CBWHAF]2.0.CO;2 en http://dx.doi.org/10.1603/0013-8746(2000)093[0573:CBWHAF]2.0.CO;2 none 4085 bytes image/gif Entomological Society of America |
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Bemisia tabaci Feeding behavior Tomato yellow leaf curl virus Transmission Bemisia tabaci Feeding behavior Tomato yellow leaf curl virus Transmission |
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Bemisia tabaci Feeding behavior Tomato yellow leaf curl virus Transmission Bemisia tabaci Feeding behavior Tomato yellow leaf curl virus Transmission Jiang, Y-X. Blas, C. de Barrios, Laura Fereres, Alberto Correlation Between Whitefly (Homoptera: Aleyrodidae) Feeding Behavior and Transmission of Tomato Yellow Leaf Curl Virus |
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The feeding behavior of the whitefly Bemisia tabaci (Gennadius) was monitored using the electrical penetration graph (EPG) technique during the transmission process of tomato yellow leaf curl geminivirus (TYLCV). The behavior of individual viruliferous whiteflies was recorded on two-leaf stage tomato test plants (Lycopersicon esculentum Mill ‘Riofuego’). A total of 213 whitefly individuals was recorded on single test plants during an inoculation access period that ranged from 3.5 to 14 h. Recordings were classified into 4 categories depending of the waveforms observed: group I, including only waveform C (pathway), was associated with a residual 2.4% TYLCV transmission efficiency; group II, showing stylet pathway and a single E(pd)1 waveform, was associated with a 7.4% transmission efficiency; group III, showing stylet pathway and a single E(pd)1 + E(pd)2 waveform, achieved a 23.4% transmission efficiency; and finally group IV, showing pathway followed by several E(pd)1 + E(pd)2 waveforms, was associated with the highest transmission efficiency (37.5%). A total of 16 different behavioral variables was introduced into a stepwise-backward logistic regression model to determine the variables most related to TYLCV inoculation. Among them, the total duration of E(pd)1 was the most significant variable associated with virus inoculation by B. tabaci (P = 0.002, positive relationship). In addition, the regression analysis indicated a strong positive relationship (P = 0.005) between transmission efficiency and total number of E(pd)1 (t = 4.39, β = 0.45 ± 0.10, r = 0.87), and total duration of waveform E(pd)1 (P = 0.05) (t = 4.00, β = 0.02 ± 0.004, r = 0.94). The minimum phloem contact E(pd)1 threshold period observed for successful inoculation of TYLCV by B. tabaci on tomato plants was 1.8 min. Implications of these results for persistent virus transmission by whiteflies are discussed. |
format |
artículo |
topic_facet |
Bemisia tabaci Feeding behavior Tomato yellow leaf curl virus Transmission |
author |
Jiang, Y-X. Blas, C. de Barrios, Laura Fereres, Alberto |
author_facet |
Jiang, Y-X. Blas, C. de Barrios, Laura Fereres, Alberto |
author_sort |
Jiang, Y-X. |
title |
Correlation Between Whitefly (Homoptera: Aleyrodidae) Feeding Behavior and Transmission of Tomato Yellow Leaf Curl Virus |
title_short |
Correlation Between Whitefly (Homoptera: Aleyrodidae) Feeding Behavior and Transmission of Tomato Yellow Leaf Curl Virus |
title_full |
Correlation Between Whitefly (Homoptera: Aleyrodidae) Feeding Behavior and Transmission of Tomato Yellow Leaf Curl Virus |
title_fullStr |
Correlation Between Whitefly (Homoptera: Aleyrodidae) Feeding Behavior and Transmission of Tomato Yellow Leaf Curl Virus |
title_full_unstemmed |
Correlation Between Whitefly (Homoptera: Aleyrodidae) Feeding Behavior and Transmission of Tomato Yellow Leaf Curl Virus |
title_sort |
correlation between whitefly (homoptera: aleyrodidae) feeding behavior and transmission of tomato yellow leaf curl virus |
publisher |
Entomological Society of America |
publishDate |
2000-05 |
url |
http://hdl.handle.net/10261/11467 |
work_keys_str_mv |
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_version_ |
1777663554992734208 |