Two consecutive Wolbachia-mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae)
Swallowtail butterflies (Papilionidae) are among the most spectacular and well-known Lepidoptera in the European fauna, but their systematics is not fully elucidated. A notable case is that of Iphiclides feisthamelii which, after more than 180 years since description, still has a debated status, being often considered as a subspecies of Iphiclides podalirius. To elucidate the relationship between the two taxa and the evolutionary processes that led to their separation, we combine mitochondrial and nuclear DNA (mtDNA and nDNA) data, Wolbachia screening, genitalia morphology and wing UV reflectance. Our results show that the two taxa clearly differ in male and female genital morphology, male wing UV reflectance and nDNA. Two Wolbachia strains were found to widely infect the studied samples, apparently explaining the phylogeographic pattern displayed by mtDNA. The available data point towards a historical Wolbachia infection that spread from I. podalirius to I. feisthamelii and produced a mitochondrial introgression. Currently, a new Wolbachia strain is spreading across mainland populations of I. podalirius, mediating once more a mitochondrial genetic sweep, which has already infected and introgressed I. feisthamelii populations in south-eastern France. We conclude that, given the marked differences in morphology and nDNA between the two taxa, and the apparent restriction of hybridization to a narrow contact area where non-hybrid specimens are common, the taxon feisthamelii should be considered as a separate species. Within this species, two well-differentiated nDNA lineages that represent European and Maghrebian populations are documented, here proposed as subspecies. The case of, presumably, two consecutive Wolbachia-mediated mitochondrial introgression events, further supports the view that infection by this endosymbiont may be frequently related to mito-nuclear discordance in insects.
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John Wiley & Sons
2019-07
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Subjects: | Cryptic species, Genetic introgression, Lepidoptera, Mito‐nuclear discordance, Systematics, Wolbachia infection, |
Online Access: | http://hdl.handle.net/10261/207683 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 |
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dig-ibe-es-10261-2076832020-12-13T00:13:00Z Two consecutive Wolbachia-mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae) Gaunet, Aurélien Dincă, Vlad Dapporto, Leonardo Montagud, Sergio Vodă, Raluca Schär, Sami Badiane, Arnaud Font, Enrique Vila, Roger European Commission Ministerio de Economía y Competitividad (España) Cryptic species Genetic introgression Lepidoptera Mito‐nuclear discordance Systematics Wolbachia infection Swallowtail butterflies (Papilionidae) are among the most spectacular and well-known Lepidoptera in the European fauna, but their systematics is not fully elucidated. A notable case is that of Iphiclides feisthamelii which, after more than 180 years since description, still has a debated status, being often considered as a subspecies of Iphiclides podalirius. To elucidate the relationship between the two taxa and the evolutionary processes that led to their separation, we combine mitochondrial and nuclear DNA (mtDNA and nDNA) data, Wolbachia screening, genitalia morphology and wing UV reflectance. Our results show that the two taxa clearly differ in male and female genital morphology, male wing UV reflectance and nDNA. Two Wolbachia strains were found to widely infect the studied samples, apparently explaining the phylogeographic pattern displayed by mtDNA. The available data point towards a historical Wolbachia infection that spread from I. podalirius to I. feisthamelii and produced a mitochondrial introgression. Currently, a new Wolbachia strain is spreading across mainland populations of I. podalirius, mediating once more a mitochondrial genetic sweep, which has already infected and introgressed I. feisthamelii populations in south-eastern France. We conclude that, given the marked differences in morphology and nDNA between the two taxa, and the apparent restriction of hybridization to a narrow contact area where non-hybrid specimens are common, the taxon feisthamelii should be considered as a separate species. Within this species, two well-differentiated nDNA lineages that represent European and Maghrebian populations are documented, here proposed as subspecies. The case of, presumably, two consecutive Wolbachia-mediated mitochondrial introgression events, further supports the view that infection by this endosymbiont may be frequently related to mito-nuclear discordance in insects. European Regional Development Fund. Spanish Agencia Estatal de Investigación (AEI). Grant Number: CGL2016-76322. Marie Skłodowska‐Curie. Grant Numbers: 609402-2020, 625997. Peer reviewed 2020-04-15T12:32:53Z 2020-04-15T12:32:53Z 2019-07 2020-04-15T12:32:53Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1111/zsc.12355 e-issn: 1463-6409 issn: 0300-3256 Zoologica Scripta 48(4): 507-519 (2019) http://hdl.handle.net/10261/207683 10.1111/zsc.12355 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2016-76322-P info:eu-repo/grantAgreement/EC/H2020/609402 info:eu-repo/grantAgreement/EC/FP7/625997 Postprint http://doi.org/10.1111/zsc.12355 Sí open John Wiley & Sons |
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Cryptic species Genetic introgression Lepidoptera Mito‐nuclear discordance Systematics Wolbachia infection Cryptic species Genetic introgression Lepidoptera Mito‐nuclear discordance Systematics Wolbachia infection |
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Cryptic species Genetic introgression Lepidoptera Mito‐nuclear discordance Systematics Wolbachia infection Cryptic species Genetic introgression Lepidoptera Mito‐nuclear discordance Systematics Wolbachia infection Gaunet, Aurélien Dincă, Vlad Dapporto, Leonardo Montagud, Sergio Vodă, Raluca Schär, Sami Badiane, Arnaud Font, Enrique Vila, Roger Two consecutive Wolbachia-mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae) |
description |
Swallowtail butterflies (Papilionidae) are among the most spectacular and well-known Lepidoptera in the European fauna, but their systematics is not fully elucidated. A notable case is that of Iphiclides feisthamelii which, after more than 180 years since description, still has a debated status, being often considered as a subspecies of Iphiclides podalirius. To elucidate the relationship between the two taxa and the evolutionary processes that led to their separation, we combine mitochondrial and nuclear DNA (mtDNA and nDNA) data, Wolbachia screening, genitalia morphology and wing UV reflectance. Our results show that the two taxa clearly differ in male and female genital morphology, male wing UV reflectance and nDNA. Two Wolbachia strains were found to widely infect the studied samples, apparently explaining the phylogeographic pattern displayed by mtDNA. The available data point towards a historical Wolbachia infection that spread from I. podalirius to I. feisthamelii and produced a mitochondrial introgression. Currently, a new Wolbachia strain is spreading across mainland populations of I. podalirius, mediating once more a mitochondrial genetic sweep, which has already infected and introgressed I. feisthamelii populations in south-eastern France. We conclude that, given the marked differences in morphology and nDNA between the two taxa, and the apparent restriction of hybridization to a narrow contact area where non-hybrid specimens are common, the taxon feisthamelii should be considered as a separate species. Within this species, two well-differentiated nDNA lineages that represent European and Maghrebian populations are documented, here proposed as subspecies. The case of, presumably, two consecutive Wolbachia-mediated mitochondrial introgression events, further supports the view that infection by this endosymbiont may be frequently related to mito-nuclear discordance in insects. |
author2 |
European Commission |
author_facet |
European Commission Gaunet, Aurélien Dincă, Vlad Dapporto, Leonardo Montagud, Sergio Vodă, Raluca Schär, Sami Badiane, Arnaud Font, Enrique Vila, Roger |
format |
artículo |
topic_facet |
Cryptic species Genetic introgression Lepidoptera Mito‐nuclear discordance Systematics Wolbachia infection |
author |
Gaunet, Aurélien Dincă, Vlad Dapporto, Leonardo Montagud, Sergio Vodă, Raluca Schär, Sami Badiane, Arnaud Font, Enrique Vila, Roger |
author_sort |
Gaunet, Aurélien |
title |
Two consecutive Wolbachia-mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae) |
title_short |
Two consecutive Wolbachia-mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae) |
title_full |
Two consecutive Wolbachia-mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae) |
title_fullStr |
Two consecutive Wolbachia-mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae) |
title_full_unstemmed |
Two consecutive Wolbachia-mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae) |
title_sort |
two consecutive wolbachia-mediated mitochondrial introgressions obscure taxonomy in palearctic swallowtail butterflies (lepidoptera, papilionidae) |
publisher |
John Wiley & Sons |
publishDate |
2019-07 |
url |
http://hdl.handle.net/10261/207683 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 |
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