Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types
[Background]: Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan species. [Results]: We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined. Data were compared with equivalent data for two long germ-band holometabolan species Drosophila melanogaster and Drosophila virilis, and the short germ-band holometabolan species Tribolium castaneum. The identification of B. germanica embryo small RNA sequences unveiled miRNAs not detected in previous studies, such as those of the MIR-309 family and 54 novel miRNAs. Four main waves of miRNA expression were recognized (with most miRNA changes occurring during the embryonic stages): the first from day 0 to day 1 of embryogenesis, the second during mid-embryogenesis (days 0-6), the third (with an acute expression peak) on day 2 of embryonic development, and the fourth during post-embryonic development. The second wave defined the boundaries of maternal-to-zygotic transition, with maternal mRNAs being cleared, presumably by Mir-309 and associated scavenger miRNAs. [Conclusion]: miRNAs follow well-defined patterns of expression over hemimetabolan ontogeny, patterns that are more diverse during embryonic development than during the nymphal stages. The results suggest that miRNAs play important roles in the developmental transitions between the embryonic stages of development (starting with maternal loading), during which they might influence the germ-band type and metamorphosis mode.
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BioMed Central
2017
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Online Access: | http://hdl.handle.net/10261/161178 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 |
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dig-ibe-es-10261-1611782021-12-28T15:51:47Z Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types Ylla, Guillem Piulachs, Maria-Dolors Bellés, Xavier Ministerio de Economía y Competitividad (España) European Commission Ministerio de Ciencia e Innovación (España) Generalitat de Catalunya CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI) [Background]: Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan species. [Results]: We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined. Data were compared with equivalent data for two long germ-band holometabolan species Drosophila melanogaster and Drosophila virilis, and the short germ-band holometabolan species Tribolium castaneum. The identification of B. germanica embryo small RNA sequences unveiled miRNAs not detected in previous studies, such as those of the MIR-309 family and 54 novel miRNAs. Four main waves of miRNA expression were recognized (with most miRNA changes occurring during the embryonic stages): the first from day 0 to day 1 of embryogenesis, the second during mid-embryogenesis (days 0-6), the third (with an acute expression peak) on day 2 of embryonic development, and the fourth during post-embryonic development. The second wave defined the boundaries of maternal-to-zygotic transition, with maternal mRNAs being cleared, presumably by Mir-309 and associated scavenger miRNAs. [Conclusion]: miRNAs follow well-defined patterns of expression over hemimetabolan ontogeny, patterns that are more diverse during embryonic development than during the nymphal stages. The results suggest that miRNAs play important roles in the developmental transitions between the embryonic stages of development (starting with maternal loading), during which they might influence the germ-band type and metamorphosis mode. This work was supported by the Spanish Ministry of Economy and Competitiveness (grants CGL2012–36251 and CGL2015–64727-P to X.B.), the Spanish Ministry of Science and Innovation (grants BFU2011–22404 and CGL2016–76011-R to M.D.P.) and the Catalan Government (2014 SGR 619). It also received financial assistance from the European Fund for Economic and Regional Development (FEDER funds to X.B. and M.D.P.). We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Peer Reviewed 2018-02-22T10:24:42Z 2018-02-22T10:24:42Z 2017 2018-02-22T10:24:42Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1186/s12864-017-4177-5 e-issn: 1471-2164 BMC Genomics 18(1): 774 (2017) http://hdl.handle.net/10261/161178 10.1186/s12864-017-4177-5 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 29020923 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2016–76011-R info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2015–64727-P Publisher's version https://doi.org/10.1186/s12864-017-4177-5 Sí open BioMed Central |
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[Background]: Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan species. [Results]: We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined. Data were compared with equivalent data for two long germ-band holometabolan species Drosophila melanogaster and Drosophila virilis, and the short germ-band holometabolan species Tribolium castaneum. The identification of B. germanica embryo small RNA sequences unveiled miRNAs not detected in previous studies, such as those of the MIR-309 family and 54 novel miRNAs. Four main waves of miRNA expression were recognized (with most miRNA changes occurring during the embryonic stages): the first from day 0 to day 1 of embryogenesis, the second during mid-embryogenesis (days 0-6), the third (with an acute expression peak) on day 2 of embryonic development, and the fourth during post-embryonic development. The second wave defined the boundaries of maternal-to-zygotic transition, with maternal mRNAs being cleared, presumably by Mir-309 and associated scavenger miRNAs. [Conclusion]: miRNAs follow well-defined patterns of expression over hemimetabolan ontogeny, patterns that are more diverse during embryonic development than during the nymphal stages. The results suggest that miRNAs play important roles in the developmental transitions between the embryonic stages of development (starting with maternal loading), during which they might influence the germ-band type and metamorphosis mode. |
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Ministerio de Economía y Competitividad (España) |
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Ministerio de Economía y Competitividad (España) Ylla, Guillem Piulachs, Maria-Dolors Bellés, Xavier |
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Ylla, Guillem Piulachs, Maria-Dolors Bellés, Xavier |
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Ylla, Guillem Piulachs, Maria-Dolors Bellés, Xavier Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types |
author_sort |
Ylla, Guillem |
title |
Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types |
title_short |
Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types |
title_full |
Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types |
title_fullStr |
Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types |
title_full_unstemmed |
Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types |
title_sort |
comparative analysis of mirna expression during the development of insects of different metamorphosis modes and germ-band types |
publisher |
BioMed Central |
publishDate |
2017 |
url |
http://hdl.handle.net/10261/161178 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 |
work_keys_str_mv |
AT yllaguillem comparativeanalysisofmirnaexpressionduringthedevelopmentofinsectsofdifferentmetamorphosismodesandgermbandtypes AT piulachsmariadolors comparativeanalysisofmirnaexpressionduringthedevelopmentofinsectsofdifferentmetamorphosismodesandgermbandtypes AT bellesxavier comparativeanalysisofmirnaexpressionduringthedevelopmentofinsectsofdifferentmetamorphosismodesandgermbandtypes |
_version_ |
1777668729815957504 |