A single miRNA drives hemimetabolan metamophosis to a right end

A single miRNA drives hemimetabolan metamophosis to a right end

Trabajo presentado en el Insect Hormones (19th Ecdysone) International Workshop, celebrado en Minneapolis del 21 al 26 de julio de 2013.

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Main Authors: Bellés, Xavier, Lozano Fernández, Jesús, Montañez, Raúl
Format: comunicación de congreso biblioteca
Language:English
Published: 2013-07
Online Access:http://hdl.handle.net/10261/153624
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spelling dig-ibe-es-10261-1536242020-07-27T07:47:30Z A single miRNA drives hemimetabolan metamophosis to a right end Bellés, Xavier Lozano Fernández, Jesús Montañez, Raúl Trabajo presentado en el Insect Hormones (19th Ecdysone) International Workshop, celebrado en Minneapolis del 21 al 26 de julio de 2013. Previous studies carried out in our laboratory had shown that RNAi depletion of dicer-1, a key enzyme involved in miRNA maturation, inhibited metamorphosis in the cockroach Blattella germanica. Dicer-1 suppression in the last (6th) instar nymph triggered the depletion of miRNA expression, and the nymphs molted to a supernumerary 7th nymphal instar instead to molt to adults. In order to identify which miRNAs are involved in regulating metamorphosis, we focused our attention on Krüppel-h1 (Kr-h1), a JH-dependent transcription factor that represses metamorphosis. Indeed, a decrease of Kr-h1 expression in the pre-metamorphic instar is crucial for metamorphosis progression. The decrease is abrupt, and this abruptness is hardly explained only by mechanisms controlling Kr-h1 transcription, and might be driven by miRNAs. Using again B. germanica as model, we observed that RNAi of Kr-h1 carried out in dicer-1-depleted specimens rescued normal metamorphosis. This pointed to miRNAs affecting Kr-h1 as responsible of the metamorphosis inhibition triggered by dicer-1 depletion. We also observed that the 3’UTR of Kr-h1 mRNA contains predicted sites for miR-2 family miRNAs, and that the predicted sites are functional, according to the results of luciferase reporter assays. Moreover, depletion of miR-2a impaired the decrease of Kr-h1 expression leading to metamorphosis inhibition, and administration of miR-2a mimic rescued the normal metamorphosis phenotype in dicer-1-depleted insects. The whole data suggests that miR-2a plays the key role of radically removing Kr-h1 mRNA in the premetamorphic stage, as a prerequisite for the activation of the transcription factors repressed by Kr-h1 that are the ultimate inducers of metamorphosis. No 2017-07-27T09:44:11Z 2017-07-27T09:44:11Z 2013-07 comunicación de congreso http://purl.org/coar/resource_type/c_5794 Insect Hormones (19th Ecdysone) International Workshop (2013) http://hdl.handle.net/10261/153624 en Sí none
institution IBE ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ibe-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IBE España
language English
description Trabajo presentado en el Insect Hormones (19th Ecdysone) International Workshop, celebrado en Minneapolis del 21 al 26 de julio de 2013.
format comunicación de congreso
author Bellés, Xavier
Lozano Fernández, Jesús
Montañez, Raúl
spellingShingle Bellés, Xavier
Lozano Fernández, Jesús
Montañez, Raúl
A single miRNA drives hemimetabolan metamophosis to a right end
author_facet Bellés, Xavier
Lozano Fernández, Jesús
Montañez, Raúl
author_sort Bellés, Xavier
title A single miRNA drives hemimetabolan metamophosis to a right end
title_short A single miRNA drives hemimetabolan metamophosis to a right end
title_full A single miRNA drives hemimetabolan metamophosis to a right end
title_fullStr A single miRNA drives hemimetabolan metamophosis to a right end
title_full_unstemmed A single miRNA drives hemimetabolan metamophosis to a right end
title_sort single mirna drives hemimetabolan metamophosis to a right end
publishDate 2013-07
url http://hdl.handle.net/10261/153624
work_keys_str_mv AT bellesxavier asinglemirnadriveshemimetabolanmetamophosistoarightend
AT lozanofernandezjesus asinglemirnadriveshemimetabolanmetamophosistoarightend
AT montanezraul asinglemirnadriveshemimetabolanmetamophosistoarightend
AT bellesxavier singlemirnadriveshemimetabolanmetamophosistoarightend
AT lozanofernandezjesus singlemirnadriveshemimetabolanmetamophosistoarightend
AT montanezraul singlemirnadriveshemimetabolanmetamophosistoarightend
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