Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia

Engineered synthetic biological devices have been designed to perform a variety of functions from sensing molecules and bioremediation to energy production and biomedicine. Notwithstanding, a major limitation of in vivo circuit implementation is the constraint associated to the use of standard methodologies for circuit design. Thus, future success of these devices depends on obtaining circuits with scalable complexity and reusable parts. Here we show how to build complex computational devices using multicellular consortia and space as key computational elements. This spatial modular design grants scalability since its general architecture is independent of the circuit’s complexity, minimizes wiring requirements and allows component reusability with minimal genetic engineering. The potential use of this approach is demonstrated by implementation of complex logical functions with up to six inputs, thus demonstrating the scalability and flexibility of this method. The potential implications of our results are outlined.

Bibliographic Details

Main Authors:	Macía, Javier, Manzoni, Romilde, Conde-Pueyo, Núria, Urrios, Arturo, Nadal, Eulàlia de, Solé, Ricard V., Posas, Francesc
Other Authors:	European Commission
Format:	artículo biblioteca
Language:	English
Published:	Public Library of Science 2016-02-01
Online Access:	http://hdl.handle.net/10261/151818 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100000781 http://dx.doi.org/10.13039/501100006373 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003043 http://dx.doi.org/10.13039/501100003741 http://dx.doi.org/10.13039/100010784 http://dx.doi.org/10.13039/100011419
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