Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
[Background] Chromosomal rearrangements have the potential to limit the rate and pattern of gene flow within and between species and thus play a direct role in promoting and maintaining speciation. Wood white butterflies of the genus Leptidea are excellent models to study the role of chromosome rearrangements in speciation because they show karyotype variability not only among but also within species. In this work, we investigated genome architecture of three cryptic Leptidea species (L. juvernica, L. sinapis and L. reali) by standard and molecular cytogenetic techniques in order to reveal causes of the karyotype variability.
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BioMed Central
2015-05-19
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| Subjects: | Lepidoptera, Wood white butterflies, Karyotype variability, Fluorescence in situ hybridization Chromosome fusion and fission, Multiple sex chromosomes, Speciation, |
| Online Access: | http://hdl.handle.net/10261/125559 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004240 |
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dig-ibe-es-10261-1255592021-12-27T16:22:50Z Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies Šíchová, Jindra Voleníková, Anna Dincă, Vlad Nguyen, Petr Vila, Roger Sahara, Ken Marec, František Czech Science Foundation Academy of Sciences of the Czech Republic University of South Bohemia Ministerio de Economía y Competitividad (España) European Commission Lepidoptera Wood white butterflies Karyotype variability Fluorescence in situ hybridization Chromosome fusion and fission Multiple sex chromosomes Speciation [Background] Chromosomal rearrangements have the potential to limit the rate and pattern of gene flow within and between species and thus play a direct role in promoting and maintaining speciation. Wood white butterflies of the genus Leptidea are excellent models to study the role of chromosome rearrangements in speciation because they show karyotype variability not only among but also within species. In this work, we investigated genome architecture of three cryptic Leptidea species (L. juvernica, L. sinapis and L. reali) by standard and molecular cytogenetic techniques in order to reveal causes of the karyotype variability. [Results] Chromosome numbers ranged from 2n = 85 to 91 in L. juvernica and 2n = 69 to 73 in L. sinapis (both from Czech populations) to 2n = 51 to 55 in L. reali (Spanish population). We observed significant differences in chromosome numbers and localization of cytogenetic markers (rDNA and H3 histone genes) within the offspring of individual females. Using FISH with the (TTAGG) n telomeric probe we also documented the presence of multiple chromosome fusions and/or fissions and other complex rearrangements. Thus, the intraspecific karyotype variability is likely due to irregular chromosome segregation of multivalent meiotic configurations. The analysis of female meiotic chromosomes by GISH and CGH revealed multiple sex chromosomes: W1W2W3Z1Z2Z3Z4 in L. juvernica, W1W2W3Z1Z2Z3 in L. sinapis and W1W2W3W4Z1Z2Z3Z4 in L. reali. [Conclusions] Our results suggest a dynamic karyotype evolution and point to the role of chromosomal rearrangements in the speciation of Leptidea butterflies. Moreover, our study revealed a curious sex determination system with 3–4 W and 3–4 Z chromosomes, which is unique in the Lepidoptera and which could also have played a role in the speciation process of the three Leptidea species. This research was funded by Grant 14-22765S of the Czech Science Foundation, Grant IAA600960925 of the Grant Agency of The Czech Academy of Sciences, Grant 063/2012/P of the Grant Agency of the University of South Bohemia and Grant CGL2013-48277-P from the Spanish Ministerio de Economía y Competitividad. JŠ and AV were supported by Grant 052/2013/P of the Grant Agency of the University of South Bohemia, VD by a Marie Curie International Outgoing Fellowship within the 7th European Community Framework Programme (project no. 625997), PN by Grant 14-35819P of the Czech Science Foundation and KS by JSPS 23380030 grant and JSPS Excellent Young Researchers Overseas Visit Program (21–7147). Peer reviewed 2015-11-23T16:11:28Z 2015-11-23T16:11:28Z 2015-05-19 2015-11-23T16:11:29Z artículo http://purl.org/coar/resource_type/c_6501 BMC Evolutionary Biology 15(1): 89 (2015) 1471-2148 http://hdl.handle.net/10261/125559 10.1186/s12862-015-0375-4 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004240 25981157 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2013-48277-P info:eu-repo/grantAgreement/EC/FP7/625997 Publisher's version http://dx.doi.org/10.1186/s12862-015-0375-4 Sí open BioMed Central |
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Lepidoptera Wood white butterflies Karyotype variability Fluorescence in situ hybridization Chromosome fusion and fission Multiple sex chromosomes Speciation Lepidoptera Wood white butterflies Karyotype variability Fluorescence in situ hybridization Chromosome fusion and fission Multiple sex chromosomes Speciation |
| spellingShingle |
Lepidoptera Wood white butterflies Karyotype variability Fluorescence in situ hybridization Chromosome fusion and fission Multiple sex chromosomes Speciation Lepidoptera Wood white butterflies Karyotype variability Fluorescence in situ hybridization Chromosome fusion and fission Multiple sex chromosomes Speciation Šíchová, Jindra Voleníková, Anna Dincă, Vlad Nguyen, Petr Vila, Roger Sahara, Ken Marec, František Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies |
| description |
[Background]
Chromosomal rearrangements have the potential to limit the rate and pattern of gene flow within and between species and thus play a direct role in promoting and maintaining speciation. Wood white butterflies of the genus Leptidea are excellent models to study the role of chromosome rearrangements in speciation because they show karyotype variability not only among but also within species. In this work, we investigated genome architecture of three cryptic Leptidea species (L. juvernica, L. sinapis and L. reali) by standard and molecular cytogenetic techniques in order to reveal causes of the karyotype variability. |
| author2 |
Czech Science Foundation |
| author_facet |
Czech Science Foundation Šíchová, Jindra Voleníková, Anna Dincă, Vlad Nguyen, Petr Vila, Roger Sahara, Ken Marec, František |
| format |
artículo |
| topic_facet |
Lepidoptera Wood white butterflies Karyotype variability Fluorescence in situ hybridization Chromosome fusion and fission Multiple sex chromosomes Speciation |
| author |
Šíchová, Jindra Voleníková, Anna Dincă, Vlad Nguyen, Petr Vila, Roger Sahara, Ken Marec, František |
| author_sort |
Šíchová, Jindra |
| title |
Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies |
| title_short |
Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies |
| title_full |
Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies |
| title_fullStr |
Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies |
| title_full_unstemmed |
Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies |
| title_sort |
dynamic karyotype evolution and unique sex determination systems in leptidea wood white butterflies |
| publisher |
BioMed Central |
| publishDate |
2015-05-19 |
| url |
http://hdl.handle.net/10261/125559 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004240 |
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| _version_ |
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