Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum
Bifidobacteria are one of the main microbial inhabitants of the human colon. Usually administered in fermented dairy products as beneficial microorganisms, they have to overcome the acidic pH found in the stomach during the gastrointestinal transit to be able to colonize the lower parts of the intestine. The mechanisms underlying acid response and adaptation in Bifidobacterium longum biotype longum NCIMB 8809 and its mutant resistant to acid pH B. longum biotype longum 8809dpH, were studied. Comparison of protein maps, and protein identification by MALDI-TOF mass spectrometry analysis, allowed us to identify 9 different proteins whose production largely changed in the mutant strain. Furthermore, the production of 47 proteins was modulated by pH in one or both strains. These included general stress response chaperones, proteins involved in transcription and translation, as well as in the carbohydrate and nitrogen metabolism, among others. Significant differences in the levels of metabolic end-products and in the redox status of the cells were also detected between the wild type strain and its acid pH resistant mutant in response, or as a result of, adaptation to acid. Remarkably, the results of this work indicated that adaptation and response to low pH in B. longum biotype longum involves changes in the glycolytic flux and in the ability to regulate the internal pH. These changes were accompanied by a higher content of ammonium in the cytoplasm, likely coming from amino acid deamination, and a decrease of the bile salt hydrolase activity
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American Society for Microbiology
2007-08-24
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Subjects: | Bifidobacterias, Proteinas, |
Online Access: | http://hdl.handle.net/10261/7049 http://dx.doi.org/10.13039/501100006488 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100006280 http://dx.doi.org/10.13039/501100007273 |
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dig-iata-es-10261-70492022-12-22T08:28:54Z Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum Sánchez García, Borja Champomier-Vergès, Marie-Christine Collado, María Carmen Anglade, Patricia Baraige, Fabienne Sanz Herranz, Yolanda González de los Reyes-Gavilán, Clara Margolles Barros, Abelardo Zagorec, Monique Institut National de la Recherche Agronomique (France) European Commission Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Ministerio de Ciencia y Tecnología (España) Bifidobacterias Proteinas Bifidobacteria are one of the main microbial inhabitants of the human colon. Usually administered in fermented dairy products as beneficial microorganisms, they have to overcome the acidic pH found in the stomach during the gastrointestinal transit to be able to colonize the lower parts of the intestine. The mechanisms underlying acid response and adaptation in Bifidobacterium longum biotype longum NCIMB 8809 and its mutant resistant to acid pH B. longum biotype longum 8809dpH, were studied. Comparison of protein maps, and protein identification by MALDI-TOF mass spectrometry analysis, allowed us to identify 9 different proteins whose production largely changed in the mutant strain. Furthermore, the production of 47 proteins was modulated by pH in one or both strains. These included general stress response chaperones, proteins involved in transcription and translation, as well as in the carbohydrate and nitrogen metabolism, among others. Significant differences in the levels of metabolic end-products and in the redox status of the cells were also detected between the wild type strain and its acid pH resistant mutant in response, or as a result of, adaptation to acid. Remarkably, the results of this work indicated that adaptation and response to low pH in B. longum biotype longum involves changes in the glycolytic flux and in the ability to regulate the internal pH. These changes were accompanied by a higher content of ammonium in the cytoplasm, likely coming from amino acid deamination, and a decrease of the bile salt hydrolase activity This work was financed by European Union FEDER funds and the Spanish Plan Nacional de I + D (project AGL2004-06727-C02-01/ALI and AGL2005-05788-C02-01/ALI). B. Sánchez was the recipient of a FPI predoctoral fellowship from the Spanish Ministerio de Ciencia y Tecnología and from the MICA department of the National Institute for Research in Agronomy (INRA). Peer reviewed 2008-09-02T08:27:27Z 2008-09-02T08:27:27Z 2007-08-24 artículo http://purl.org/coar/resource_type/c_6501 Applied and environmental microbiology 73 (20): 6450-6459 (2007) 0099-2240 http://hdl.handle.net/10261/7049 10.1128/AEM.00886-07 http://dx.doi.org/10.13039/501100006488 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100006280 http://dx.doi.org/10.13039/501100007273 17720838 en open 379220 bytes application/pdf American Society for Microbiology |
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Bifidobacterias Proteinas Bifidobacterias Proteinas |
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Bifidobacterias Proteinas Bifidobacterias Proteinas Sánchez García, Borja Champomier-Vergès, Marie-Christine Collado, María Carmen Anglade, Patricia Baraige, Fabienne Sanz Herranz, Yolanda González de los Reyes-Gavilán, Clara Margolles Barros, Abelardo Zagorec, Monique Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum |
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Bifidobacteria are one of the main microbial inhabitants of the human colon. Usually administered in fermented dairy products as beneficial microorganisms, they have to overcome the acidic pH found in the stomach during the gastrointestinal transit to be able to colonize the lower parts of the intestine. The mechanisms underlying acid response and adaptation in Bifidobacterium longum biotype longum NCIMB 8809 and its mutant resistant to acid pH B. longum biotype longum 8809dpH, were studied. Comparison of protein maps, and protein identification by MALDI-TOF mass spectrometry analysis, allowed us to identify 9 different proteins whose production largely changed in the mutant strain. Furthermore, the production of 47 proteins was modulated by pH in one or both strains. These included general stress response chaperones, proteins involved in transcription and translation, as well as in the carbohydrate and nitrogen metabolism, among others. Significant differences in the levels of metabolic end-products and in the redox status of the cells were also detected between the wild type strain and its acid pH resistant mutant in response, or as a result of, adaptation to acid. Remarkably, the results of this work indicated that adaptation and response to low pH in B. longum biotype longum involves changes in the glycolytic flux and in the ability to regulate the internal pH. These changes were accompanied by a higher content of ammonium in the cytoplasm, likely coming from amino acid deamination, and a decrease of the bile salt hydrolase activity |
author2 |
Institut National de la Recherche Agronomique (France) |
author_facet |
Institut National de la Recherche Agronomique (France) Sánchez García, Borja Champomier-Vergès, Marie-Christine Collado, María Carmen Anglade, Patricia Baraige, Fabienne Sanz Herranz, Yolanda González de los Reyes-Gavilán, Clara Margolles Barros, Abelardo Zagorec, Monique |
format |
artículo |
topic_facet |
Bifidobacterias Proteinas |
author |
Sánchez García, Borja Champomier-Vergès, Marie-Christine Collado, María Carmen Anglade, Patricia Baraige, Fabienne Sanz Herranz, Yolanda González de los Reyes-Gavilán, Clara Margolles Barros, Abelardo Zagorec, Monique |
author_sort |
Sánchez García, Borja |
title |
Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum |
title_short |
Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum |
title_full |
Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum |
title_fullStr |
Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum |
title_full_unstemmed |
Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum |
title_sort |
low ph adaptation and the acid tolerance response of bifidobacterium longum biotype longum |
publisher |
American Society for Microbiology |
publishDate |
2007-08-24 |
url |
http://hdl.handle.net/10261/7049 http://dx.doi.org/10.13039/501100006488 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100006280 http://dx.doi.org/10.13039/501100007273 |
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