Truncated phytases of bifidobacteria and uses thereof

Truncated phytases of bifidobacteria and uses thereof

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[EN] The invention relates to an isolated polynucleotide consisting of a nucleotide sequence coding for an amino acid sequence of Bifidobacterimn having at least 55% identity with amino acid sequence SEQ ID NO: 1, in which said amino acid sequence lacks a sequence coding for the transmembrane helix, located at the carboxy-tenninal end of the original sequence, and in which said amino acid sequence is a protein of which the main activity is phytase. Preferably, the polynucleotide originates from strains of Bifidobacterium pseudocatenulatum (B. pseudocatenulatum) ATCC279l9 or Bifidobacterium longum subsp. infantis (B. longmn subsp. infantis) ATCC15697. The invention also relates to the use of the polynucleotide, or to any of the products described in the invention, in arder to reduce the phytate content of a food or to produce myo-inositol triphosphate (InsP3).

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Bibliographic Details
Main Authors: Haros, Monika, Monedero, Vicente, Yebra, María Jesús, Tamayo Ramos, Juan Antonio
Format: solicitud de patente biblioteca
Language:Spanish / Castilian
Published: 2011-09-29
Online Access:http://hdl.handle.net/10261/51686
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spelling dig-iata-es-10261-516862020-05-26T10:25:21Z Truncated phytases of bifidobacteria and uses thereof Fitasas truncadas de bifidobacterias y sus usos Haros, Monika Monedero, Vicente Yebra, María Jesús Tamayo Ramos, Juan Antonio [EN] The invention relates to an isolated polynucleotide consisting of a nucleotide sequence coding for an amino acid sequence of Bifidobacterimn having at least 55% identity with amino acid sequence SEQ ID NO: 1, in which said amino acid sequence lacks a sequence coding for the transmembrane helix, located at the carboxy-tenninal end of the original sequence, and in which said amino acid sequence is a protein of which the main activity is phytase. Preferably, the polynucleotide originates from strains of Bifidobacterium pseudocatenulatum (B. pseudocatenulatum) ATCC279l9 or Bifidobacterium longum subsp. infantis (B. longmn subsp. infantis) ATCC15697. The invention also relates to the use of the polynucleotide, or to any of the products described in the invention, in arder to reduce the phytate content of a food or to produce myo-inositol triphosphate (InsP3). [ES] La presente invención se refiere a un polinucleótido aislado que consiste en una secuencia nucleotídica que codifica para una secuencia aminoacídica de Bifidobacterium que tiene al menos un 55% de identidad con respecto a la secuencia aminoacídica SEQ ID NO: 1, donde dicha secuencia aminoacídica carece de la secuencia que codifica para la hélice transmembrana, situada en el extremo carboxi-terminal de la secuencia original, y dicha secuencia aminoacídica es una proteina cuya actividad mayoritaria es fitasa. Dicho polinucleótido procede preferiblemente de las cepas Bifidobacterium pseudocatenulatum (en adelante B. pseudocatenulatum) ATCC27919 o de Bifidobacterium longum subsp. infantis (B. longum subsp. infantis) ATCC15697. Además, la presente invención se refiere al uso del polinucleótido, o a cualquiera de los productos descritos en la invención, para reducir el contenido de fitatos de un alimento o para producir mio-inositol trifosfato (InsP3). Peer reviewed Consejo Superior de Investigaciones Científicas (España) A1 Solicitud de patente con informe sobre el estado de la técnica 2012-06-18T08:01:39Z 2012-06-18T08:01:39Z 2011-09-29 2010-03-24 solicitud de patente WO2011117452 A1 http://hdl.handle.net/10261/51686 PCT/ES201l/070198 es open
institution IATA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-iata-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IATA España
language Spanish / Castilian
description [EN] The invention relates to an isolated polynucleotide consisting of a nucleotide sequence coding for an amino acid sequence of Bifidobacterimn having at least 55% identity with amino acid sequence SEQ ID NO: 1, in which said amino acid sequence lacks a sequence coding for the transmembrane helix, located at the carboxy-tenninal end of the original sequence, and in which said amino acid sequence is a protein of which the main activity is phytase. Preferably, the polynucleotide originates from strains of Bifidobacterium pseudocatenulatum (B. pseudocatenulatum) ATCC279l9 or Bifidobacterium longum subsp. infantis (B. longmn subsp. infantis) ATCC15697. The invention also relates to the use of the polynucleotide, or to any of the products described in the invention, in arder to reduce the phytate content of a food or to produce myo-inositol triphosphate (InsP3).
format solicitud de patente
author Haros, Monika
Monedero, Vicente
Yebra, María Jesús
Tamayo Ramos, Juan Antonio
spellingShingle Haros, Monika
Monedero, Vicente
Yebra, María Jesús
Tamayo Ramos, Juan Antonio
Truncated phytases of bifidobacteria and uses thereof
author_facet Haros, Monika
Monedero, Vicente
Yebra, María Jesús
Tamayo Ramos, Juan Antonio
author_sort Haros, Monika
title Truncated phytases of bifidobacteria and uses thereof
title_short Truncated phytases of bifidobacteria and uses thereof
title_full Truncated phytases of bifidobacteria and uses thereof
title_fullStr Truncated phytases of bifidobacteria and uses thereof
title_full_unstemmed Truncated phytases of bifidobacteria and uses thereof
title_sort truncated phytases of bifidobacteria and uses thereof
publishDate 2011-09-29
url http://hdl.handle.net/10261/51686
work_keys_str_mv AT harosmonika truncatedphytasesofbifidobacteriaandusesthereof
AT monederovicente truncatedphytasesofbifidobacteriaandusesthereof
AT yebramariajesus truncatedphytasesofbifidobacteriaandusesthereof
AT tamayoramosjuanantonio truncatedphytasesofbifidobacteriaandusesthereof
AT harosmonika fitasastruncadasdebifidobacteriasysususos
AT monederovicente fitasastruncadasdebifidobacteriasysususos
AT yebramariajesus fitasastruncadasdebifidobacteriasysususos
AT tamayoramosjuanantonio fitasastruncadasdebifidobacteriasysususos
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