Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates

Carrera Alvarado, Gisela; Toldrá Vilardell, Fidel; Mora, Leticia; Generalitat Valenciana

Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates

Excel file including: Degree of hydrolysis, the inhibition of DPP-IV of 5 enzymatic hydrolysates of chicken blood, peptide separation of 2 hydrolysates by reverse phase chromatography (RP-HPLC); assay of DPP IV activity and free amino acids composition per fraction, peptide identification by mass spectrometry in tandem, in silico analysis and DPP IV inhibitory activity for each synthetised peptide.

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Bibliographic Details
Main Authors:	Carrera Alvarado, Gisela, Toldrá Vilardell, Fidel, Mora, Leticia
Other Authors:	Generalitat Valenciana
Format:	dataset biblioteca
Published:	DIGITAL.CSIC 2024-11
Subjects:	DPP-IV inhibitor, Hypoglycemic peptides, Type 2 diabetes mellitus, Enzymatic hydrolysis, enzymatic hydrolysis, diabetes,
Online Access:	http://hdl.handle.net/10261/371666 https://doi.org/10.20350/digitalCSIC/16658
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spelling	dig-iata-es-10261-3716662024-11-13T11:04:55Z Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates Carrera Alvarado, Gisela Toldrá Vilardell, Fidel Mora, Leticia Generalitat Valenciana Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) Toldrá Vilardell, Fidel [0000-0002-9843-0193] Mora, Leticia [0000-0003-1571-5781] Mora, Leticia [lemoso@iata.csic.es] DPP-IV inhibitor Hypoglycemic peptides Type 2 diabetes mellitus Enzymatic hydrolysis enzymatic hydrolysis diabetes Excel file including: Degree of hydrolysis, the inhibition of DPP-IV of 5 enzymatic hydrolysates of chicken blood, peptide separation of 2 hydrolysates by reverse phase chromatography (RP-HPLC); assay of DPP IV activity and free amino acids composition per fraction, peptide identification by mass spectrometry in tandem, in silico analysis and DPP IV inhibitory activity for each synthetised peptide. Blood is a meat by-product rich in proteins with properties that can be improved after hydrolysis, making it a sustainable alternative for use in the generation of bioactive peptides. The objective of this study was to identify dipeptidyl peptidase IV (DPP-IV) inhibitory peptides obtained from different chicken blood hydrolysates prepared using combinations of four different enzymes. Best results were observed for AP (2% Alcalase + 5% Protana Prime) and APP (2% Alcalase + 5% Protana Prime + 3% Protana UBoost) hydrolysates obtaining inhibition values of 60.55 and 53.61%, respectively, assayed at a concentration of 10 mg/mL. Free amino acids were determined to establish the impact of exopeptidase activity in the samples. A total of 79 and 12 sequences of peptides were identified by liquid chromatography and mass spectrometry in tandem (LC-MS/MS) in AP and APP samples, respectively. Nine of the identified peptides were established as potential DPP-IV inhibitory using in silico approaches and later synthesized for confirmation. Thus, peptides GPF, IGL, and GGGW showed good DPP-IV inhibitory activity with IC50 values of 0.94, 2.22, and 2.73 mM, respectively. This study confirmed the potential of peptides obtained from chicken blood hydrolysates to be used as DPP-IV inhibitors and, therefore, in the control or modulation of type 2 diabetes. Grant PID2020-119684RB-I00 funded by MCIN/AEI/10.13039/501100011033. Grant GRISOLIAP/2020/021 de la Consellería de Innovació, Universitats, Ciència I Societat Digital de la Generalitat Valenciana (GCA) is also acknowledged. Peer reviewed 2024-11-13T08:52:45Z 2024-11-13T08:52:45Z 2024-11 dataset Carrera Alvarado, G.; Toldrá, F. ; Mora, L.;Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates;DIGITAL.CSIC;http://hdl.handle.net/10261/371666 http://hdl.handle.net/10261/371666 https://doi.org/10.20350/digitalCSIC/16658 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119684RB-I00/ES/GENERACION DE PEPTIDOS RELACIONADOS CON EL SABOR Y SU POTENCIACION EN ALIMENTOS OBTENIDOS A PARTIR DE SUBPRODUCTOS CARNICOS / Carrera-Alvarado, G.; Toldrá, F. ; Mora, L. DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates. https://doi.org/10.3390/ijms232214140. http://hdl.handle.net/10261/285637 Sí open DIGITAL.CSIC
institution	IATA ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-iata-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IATA España
topic	DPP-IV inhibitor Hypoglycemic peptides Type 2 diabetes mellitus Enzymatic hydrolysis enzymatic hydrolysis diabetes DPP-IV inhibitor Hypoglycemic peptides Type 2 diabetes mellitus Enzymatic hydrolysis enzymatic hydrolysis diabetes
spellingShingle	DPP-IV inhibitor Hypoglycemic peptides Type 2 diabetes mellitus Enzymatic hydrolysis enzymatic hydrolysis diabetes DPP-IV inhibitor Hypoglycemic peptides Type 2 diabetes mellitus Enzymatic hydrolysis enzymatic hydrolysis diabetes Carrera Alvarado, Gisela Toldrá Vilardell, Fidel Mora, Leticia Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates
description	Excel file including: Degree of hydrolysis, the inhibition of DPP-IV of 5 enzymatic hydrolysates of chicken blood, peptide separation of 2 hydrolysates by reverse phase chromatography (RP-HPLC); assay of DPP IV activity and free amino acids composition per fraction, peptide identification by mass spectrometry in tandem, in silico analysis and DPP IV inhibitory activity for each synthetised peptide.
author2	Generalitat Valenciana
author_facet	Generalitat Valenciana Carrera Alvarado, Gisela Toldrá Vilardell, Fidel Mora, Leticia
format	dataset
topic_facet	DPP-IV inhibitor Hypoglycemic peptides Type 2 diabetes mellitus Enzymatic hydrolysis enzymatic hydrolysis diabetes
author	Carrera Alvarado, Gisela Toldrá Vilardell, Fidel Mora, Leticia
author_sort	Carrera Alvarado, Gisela
title	Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates
title_short	Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates
title_full	Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates
title_fullStr	Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates
title_full_unstemmed	Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates
title_sort	data of manuscript dpp-iv inhibitory peptides gpf, igl, and gggw obtained from chicken blood hydrolysates
publisher	DIGITAL.CSIC
publishDate	2024-11
url	http://hdl.handle.net/10261/371666 https://doi.org/10.20350/digitalCSIC/16658
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_version_	1816138539801247744

Data of Manuscript DPP-IV Inhibitory Peptides GPF, IGL, and GGGW Obtained from Chicken Blood Hydrolysates

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