Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome?
Growing evidence indicates that gut and respiratory microbiota have a potential key effect on bronchiolitis, mainly caused by respiratory syncytial virus (RSV). This was a prospective study of 96 infants comparing infants with bronchiolitis (n = 57, both RSV and non-RSV associated) to a control group (n = 39). Gut (feces) and respiratory [nasopharyngeal aspirate (NPA)] microbial profiles were analyzed by 16S rRNA amplicon sequencing, and respiratory viruses were identified by PCR. Clinical data of the acute episode and follow-up during the first year after infection were recorded. Pairwise comparisons showed significant differences in the gut (R2 = 0.0639, P = 0.006) and NPA (R2 = 0.0803, P = 0.006) microbiota between cases and controls. A significantly lower gut microbial richness and an increase in the NPA microbial diversity (mainly due to an increase in Haemophilus, Streptococcus, and Neisseria) were observed in the infants with bronchiolitis, in those with the most severe symptoms, and in those who subsequently developed recurrent wheezing episodes after discharge. In NPA, the higher microbial richness differed significantly between the control group and the non-RSV bronchiolitis group (P = 0.01) and between the control group and the RSV bronchiolitis group (P = 0.001). In the gut, the richness differed significantly between the control group and the non-RSV group (P = 0.01) and between the control group and the RSV bronchiolitis group (P = 0.001), with higher diversity in the RSV group. A distinct respiratory and intestinal microbial pattern was observed in infants with bronchiolitis compared with controls. The presence of RSV was a main factor for dysbiosis. Lower gut microbial richness and increased respiratory microbial diversity were associated with respiratory morbidity during follow-up.
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American Society for Microbiology
2024-05-24
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Subjects: | Gut microbiota, Infants, Nasopharyngeal microbiota, Respiratory syncytial virus, Virus, infants, viruses, intestines, |
Online Access: | http://hdl.handle.net/10261/360767 |
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Gut microbiota Infants Nasopharyngeal microbiota Respiratory syncytial virus Virus infants Respiratory syncytial virus viruses intestines Gut microbiota Infants Nasopharyngeal microbiota Respiratory syncytial virus Virus infants Respiratory syncytial virus viruses intestines |
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Gut microbiota Infants Nasopharyngeal microbiota Respiratory syncytial virus Virus infants Respiratory syncytial virus viruses intestines Gut microbiota Infants Nasopharyngeal microbiota Respiratory syncytial virus Virus infants Respiratory syncytial virus viruses intestines Cabrera Rubio, Raul Calvo, Cristina Alcolea, Sonia Bergia, María Atucha, Jorge Pozo, Francisco Casas, Inmaculada Arroyas, María Collado, María Carmen García-García, María Luz Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? |
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Growing evidence indicates that gut and respiratory microbiota have a potential key effect on bronchiolitis, mainly caused by respiratory syncytial virus (RSV). This was a prospective study of 96 infants comparing infants with bronchiolitis (n = 57, both RSV and non-RSV associated) to a control group (n = 39). Gut (feces) and respiratory [nasopharyngeal aspirate (NPA)] microbial profiles were analyzed by 16S rRNA amplicon sequencing, and respiratory viruses were identified by PCR. Clinical data of the acute episode and follow-up during the first year after infection were recorded. Pairwise comparisons showed significant differences in the gut (R2 = 0.0639, P = 0.006) and NPA (R2 = 0.0803, P = 0.006) microbiota between cases and controls. A significantly lower gut microbial richness and an increase in the NPA microbial diversity (mainly due to an increase in Haemophilus, Streptococcus, and Neisseria) were observed in the infants with bronchiolitis, in those with the most severe symptoms, and in those who subsequently developed recurrent wheezing episodes after discharge. In NPA, the higher microbial richness differed significantly between the control group and the non-RSV bronchiolitis group (P = 0.01) and between the control group and the RSV bronchiolitis group (P = 0.001). In the gut, the richness differed significantly between the control group and the non-RSV group (P = 0.01) and between the control group and the RSV bronchiolitis group (P = 0.001), with higher diversity in the RSV group. A distinct respiratory and intestinal microbial pattern was observed in infants with bronchiolitis compared with controls. The presence of RSV was a main factor for dysbiosis. Lower gut microbial richness and increased respiratory microbial diversity were associated with respiratory morbidity during follow-up. |
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Generalitat Valenciana |
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Generalitat Valenciana Cabrera Rubio, Raul Calvo, Cristina Alcolea, Sonia Bergia, María Atucha, Jorge Pozo, Francisco Casas, Inmaculada Arroyas, María Collado, María Carmen García-García, María Luz |
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Gut microbiota Infants Nasopharyngeal microbiota Respiratory syncytial virus Virus infants Respiratory syncytial virus viruses intestines |
author |
Cabrera Rubio, Raul Calvo, Cristina Alcolea, Sonia Bergia, María Atucha, Jorge Pozo, Francisco Casas, Inmaculada Arroyas, María Collado, María Carmen García-García, María Luz |
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Cabrera Rubio, Raul |
title |
Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? |
title_short |
Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? |
title_full |
Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? |
title_fullStr |
Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? |
title_full_unstemmed |
Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? |
title_sort |
gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? |
publisher |
American Society for Microbiology |
publishDate |
2024-05-24 |
url |
http://hdl.handle.net/10261/360767 |
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1802820568575115264 |
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dig-iata-es-10261-3607672024-06-18T11:15:37Z Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? Cabrera Rubio, Raul Calvo, Cristina Alcolea, Sonia Bergia, María Atucha, Jorge Pozo, Francisco Casas, Inmaculada Arroyas, María Collado, María Carmen García-García, María Luz Generalitat Valenciana Fundación Universidad Alfonso X el Sabio Merck Sharp & Dohme Instituto de Salud Carlos III Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Cabrera Rubio, Raul [0000-0003-3652-9558] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Gut microbiota Infants Nasopharyngeal microbiota Respiratory syncytial virus Virus infants Respiratory syncytial virus viruses intestines Growing evidence indicates that gut and respiratory microbiota have a potential key effect on bronchiolitis, mainly caused by respiratory syncytial virus (RSV). This was a prospective study of 96 infants comparing infants with bronchiolitis (n = 57, both RSV and non-RSV associated) to a control group (n = 39). Gut (feces) and respiratory [nasopharyngeal aspirate (NPA)] microbial profiles were analyzed by 16S rRNA amplicon sequencing, and respiratory viruses were identified by PCR. Clinical data of the acute episode and follow-up during the first year after infection were recorded. Pairwise comparisons showed significant differences in the gut (R2 = 0.0639, P = 0.006) and NPA (R2 = 0.0803, P = 0.006) microbiota between cases and controls. A significantly lower gut microbial richness and an increase in the NPA microbial diversity (mainly due to an increase in Haemophilus, Streptococcus, and Neisseria) were observed in the infants with bronchiolitis, in those with the most severe symptoms, and in those who subsequently developed recurrent wheezing episodes after discharge. In NPA, the higher microbial richness differed significantly between the control group and the non-RSV bronchiolitis group (P = 0.01) and between the control group and the RSV bronchiolitis group (P = 0.001). In the gut, the richness differed significantly between the control group and the non-RSV group (P = 0.01) and between the control group and the RSV bronchiolitis group (P = 0.001), with higher diversity in the RSV group. A distinct respiratory and intestinal microbial pattern was observed in infants with bronchiolitis compared with controls. The presence of RSV was a main factor for dysbiosis. Lower gut microbial richness and increased respiratory microbial diversity were associated with respiratory morbidity during follow-up. This study was partially funded by X Convocatoria proyectos de investigación, Fundación UAX. 2018, Grant SEICAP: Convocatoria de Becas de Investigación SEICAP, Curso 2018-19, Grant SENP 2018 (Beca Senior de Investigación), Grant SEPAR 2020, Ayuda a la investigación categoría de neumología pediátrica,. Investigator Studies Program (MISP) Merck Sharp & Dohme Corp. The opinions correspond to the investigators and do not necessarily represent MSD. Funding was provided by FIS (Fondo de Investigaciones Sanitarias)–Spanish Health Research Fund-FONDOS FEDER Grants: PI18CIII/00009 and PI18/00167; PI21/00377. R.C.-R. thanks Generalitat-Valenciana (GVA) for the grant Plan GenT-Talent Attraction program (CDEIGENT 2020). M.C.C. would like to acknowledge the support from the Spanish Ministry of Science and Innovation (MCIN) research grant (ref. PID2022-139475OB-I00) and also from PROMETEO-GVA grant for Excellence Research Groups (NEOHEALTH ref.012/2020). M.C.C. and R.C.-R. would also acknowledge the award of the Spanish Government MCIN/AEI to the Institute of Agrochemistry and Food Technology (IATA-CSIC) as Centre of Excellence Severo Ochoa (CEX2021-001189-S MCIN/AEI/10.13039/501100011033). With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX 2021-001189-S) Peer reviewed 2024-06-18T11:15:37Z 2024-06-18T11:15:37Z 2024-05-24 artículo Microbiology Spectrum 24: e0255623 (2024) CEX2021-001189-S http://hdl.handle.net/10261/360767 10.1128/spectrum.02556-23 2165-0497 38785596 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-139475OB-I00/ES/EL IMPACTO DEL MICROBIOMA Y DIETA EN LA PROGRAMACION, CRECIMIENTO Y DESARROLLO DE LA SALUD INFANTIL: SEGUIMIENTO 6 AÑOS DE LA COHORTE MAMI/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX 2021-001189-S Microbiology spectrum Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1128/spectrum.02556-23 https://doi.org/10.1128/spectrum.02556-23 Sí embargo_20241124 application/pdf American Society for Microbiology |