Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water
Vegetables are globally associated with a considerable number of foodborne outbreaks caused by viral infections, specifically human norovirus. In fresh produce industry, washing represents a critical step for food safety as process wash water (PWW) needs to be maintained at appropriate microbial quality to prevent water-mediated cross-contamination. This study aimed to explore the disinfection efficacy of chlorine (free chlorine, FC), chlorine dioxide (ClO2) and peracetic acid (PAA) in PWW against infectious human norovirus and Tulane virus (TV). First, we tested the extent of TV inactivation in baby leaf, bell pepper, and vegetables mix PWW and monitored the viral decay by cell culture. Then, inactivation kinetics were defined for infectious human norovirus exposed to FC, ClO2 and PAA in baby leaves PWW using the human intestinal enteroids (HIE) system. Finally, kinetic inactivation models were fitted to TV reduction and decay of sanitizers to aid the implementation of disinfection strategies. Results showed that >8 log10 human norovirus and 3.9 log10 TV were inactivated by 20 ppm FC within 1 min; and by 3 ppm ClO2 in 1 min (TV) or 5 min (norovirus). PAA treatment at 80 ppm reduced ca. 2 log10 TV but not completely inactivated the virus even after 20 min exposure, while 5 min treatment prevented norovirus replication in HIE. TV inactivation in PWWs was described using an exponential decay model. Taking these data together, we demonstrated the value of applying the HIE model to validate current operational limits for the most commonly used sanitizers. The inactivation kinetics for human norovirus and TV, along with the predictive model described in this study expand the current knowledge to implement post-harvest produce safety procedures in industry settings.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2024-01-26
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Subjects: | Disinfection treatment, Fecal cross-contamination, Food safety, Human norovirus, Inactivation model, |
Online Access: | http://hdl.handle.net/10261/345266 |
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dig-iata-es-10261-3452662024-02-05T08:35:01Z Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water Allende, Ana Férez-Rubio, José Antonio Tudela, Juan Antonio Aznar, Rosa Gil, Maria Isabel Sánchez, Gloria Randazzo, Walter Center for Produce Safety (US) Agencia Estatal de Investigación (España) Disinfection treatment Fecal cross-contamination Food safety Human norovirus Inactivation model Vegetables are globally associated with a considerable number of foodborne outbreaks caused by viral infections, specifically human norovirus. In fresh produce industry, washing represents a critical step for food safety as process wash water (PWW) needs to be maintained at appropriate microbial quality to prevent water-mediated cross-contamination. This study aimed to explore the disinfection efficacy of chlorine (free chlorine, FC), chlorine dioxide (ClO2) and peracetic acid (PAA) in PWW against infectious human norovirus and Tulane virus (TV). First, we tested the extent of TV inactivation in baby leaf, bell pepper, and vegetables mix PWW and monitored the viral decay by cell culture. Then, inactivation kinetics were defined for infectious human norovirus exposed to FC, ClO2 and PAA in baby leaves PWW using the human intestinal enteroids (HIE) system. Finally, kinetic inactivation models were fitted to TV reduction and decay of sanitizers to aid the implementation of disinfection strategies. Results showed that >8 log10 human norovirus and 3.9 log10 TV were inactivated by 20 ppm FC within 1 min; and by 3 ppm ClO2 in 1 min (TV) or 5 min (norovirus). PAA treatment at 80 ppm reduced ca. 2 log10 TV but not completely inactivated the virus even after 20 min exposure, while 5 min treatment prevented norovirus replication in HIE. TV inactivation in PWWs was described using an exponential decay model. Taking these data together, we demonstrated the value of applying the HIE model to validate current operational limits for the most commonly used sanitizers. The inactivation kinetics for human norovirus and TV, along with the predictive model described in this study expand the current knowledge to implement post-harvest produce safety procedures in industry settings. This work was supported by the 2020CPS09 project awarded to MIG, GS, and AA and funded by the Center for Produce Safety, and by the PID2019-105509RJ-I00 project funded by MICINN/AEI to WR. IATA-CSIC is a Centre of Excellence Severo Ochoa (CEX2021-001189-S MCIN/AEI/10.13039/501100011033) With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX 2021-001189-S) Peer reviewed 2024-02-05T08:35:00Z 2024-02-05T08:35:00Z 2024-01-26 artículo International Journal of Food Microbiology 413: 110601 (2024) CEX2021-001189-S http://hdl.handle.net/10261/345266 10.1016/j.ijfoodmicro.2024.110601 3830-1540 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105509RJ-I00/ES/ESTRATEGIA PARA PREVENIR LA CONTAMINACION DE NOROVIRUS EN LA CADENA ALIMENTARIA/ International journal of food microbiology Publisher's version https://doi.org/10.1016/j.ijfoodmicro.2024.110601 Sí open Elsevier |
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Disinfection treatment Fecal cross-contamination Food safety Human norovirus Inactivation model Disinfection treatment Fecal cross-contamination Food safety Human norovirus Inactivation model |
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Disinfection treatment Fecal cross-contamination Food safety Human norovirus Inactivation model Disinfection treatment Fecal cross-contamination Food safety Human norovirus Inactivation model Allende, Ana Férez-Rubio, José Antonio Tudela, Juan Antonio Aznar, Rosa Gil, Maria Isabel Sánchez, Gloria Randazzo, Walter Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water |
description |
Vegetables are globally associated with a considerable number of foodborne outbreaks caused by viral infections, specifically human norovirus. In fresh produce industry, washing represents a critical step for food safety as process wash water (PWW) needs to be maintained at appropriate microbial quality to prevent water-mediated cross-contamination. This study aimed to explore the disinfection efficacy of chlorine (free chlorine, FC), chlorine dioxide (ClO2) and peracetic acid (PAA) in PWW against infectious human norovirus and Tulane virus (TV). First, we tested the extent of TV inactivation in baby leaf, bell pepper, and vegetables mix PWW and monitored the viral decay by cell culture. Then, inactivation kinetics were defined for infectious human norovirus exposed to FC, ClO2 and PAA in baby leaves PWW using the human intestinal enteroids (HIE) system. Finally, kinetic inactivation models were fitted to TV reduction and decay of sanitizers to aid the implementation of disinfection strategies. Results showed that >8 log10 human norovirus and 3.9 log10 TV were inactivated by 20 ppm FC within 1 min; and by 3 ppm ClO2 in 1 min (TV) or 5 min (norovirus). PAA treatment at 80 ppm reduced ca. 2 log10 TV but not completely inactivated the virus even after 20 min exposure, while 5 min treatment prevented norovirus replication in HIE. TV inactivation in PWWs was described using an exponential decay model. Taking these data together, we demonstrated the value of applying the HIE model to validate current operational limits for the most commonly used sanitizers. The inactivation kinetics for human norovirus and TV, along with the predictive model described in this study expand the current knowledge to implement post-harvest produce safety procedures in industry settings. |
author2 |
Center for Produce Safety (US) |
author_facet |
Center for Produce Safety (US) Allende, Ana Férez-Rubio, José Antonio Tudela, Juan Antonio Aznar, Rosa Gil, Maria Isabel Sánchez, Gloria Randazzo, Walter |
format |
artículo |
topic_facet |
Disinfection treatment Fecal cross-contamination Food safety Human norovirus Inactivation model |
author |
Allende, Ana Férez-Rubio, José Antonio Tudela, Juan Antonio Aznar, Rosa Gil, Maria Isabel Sánchez, Gloria Randazzo, Walter |
author_sort |
Allende, Ana |
title |
Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water |
title_short |
Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water |
title_full |
Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water |
title_fullStr |
Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water |
title_full_unstemmed |
Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water |
title_sort |
human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water |
publisher |
Elsevier |
publishDate |
2024-01-26 |
url |
http://hdl.handle.net/10261/345266 |
work_keys_str_mv |
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1792484871172521984 |