Identification of dipeptides by MALDI-ToF mass spectrometry in long-processing Spanish dry-cured ham
The processing of dry-cured ham results in the generation of small peptides by the action of endogenous enzymes on muscle proteins. Common proteomic workflows involve previous separation techniques based on liquid chromatography which are expensive and time-consuming. In this study, a convenient proteomic approach based on MALDI-ToF is proposed for the first time for the detection of dipeptides in Spanish dry-cured ham. Dipeptides AH, AL, DD, EV, and VF were identified in hams of 18 and 24 months of dry-curing. This work provides insights on the efficiency of a new peptidomic workflow for the short peptide identification from a complex food matrix and permits to evaluate the sample in terms of the presence of taste-related and bioactive dipeptides.
Main Authors: | Heres, Alejandro, Saldaña, Celia, Toldrá Vilardell, Fidel, Mora, Leticia |
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Other Authors: | Ministerio de Ciencia e Innovación (España) |
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2021-12-30
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Subjects: | Bioactivity, Dipeptides, Dry-cured ham, MALDI-ToF, Peptidomics, Taste, |
Online Access: | http://hdl.handle.net/10261/258128 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85122641712 |
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