NOD1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet
The contribution of the nucleotide-binding oligomerization domain protein NOD1 to obesity has been investigated in mice fed a high fat diet (HFD). Absence of NOD1 accelerates obesity as early as 2 weeks after feeding a HFD. The obesity was due to increases in abdominal and inguinal adipose tissues. Analysis of the resting energy expenditure showed an impaired function in NOD1-deficient animals, compatible with an alteration in thyroid hormone homeostasis. Interestingly, free thyroidal T4 increased in NOD1-deficient mice fed a HFD and the expression levels of UCP1 in brown adipose tissue were significantly lower in NOD1-deficient mice than in the wild type animals eating a HFD, thus contributing to the observed adiposity in NOD1-deficient mice. Feeding a HFD resulted in an alteration of the proinflammatory profile of these animals, with an increase in the infiltration of inflammatory cells in the liver and in the white adipose tissue, and an elevation of the circulating levels of TNF-α. In addition, alterations in the gut microbiota in NOD1-deficient mice correlate with increased vulnerability of their ecosystem to the HFD challenge and affect the immune-metabolic phenotype of obese mice. Together, the data are compatible with a protective function of NOD1 against low-grade inflammation and obesity under nutritional conditions enriched in saturated lipids. Moreover, one of the key players of this early obesity onset is a dysregulation in the metabolism and release of thyroid hormones leading to reduced energy expenditure, which represents a new role for these hormones in the metabolic actions controlled by NOD1.
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Springer Nature
2020-07-23
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Subjects: | Nucleotide-binding oligomerization domain protein, Obesity, High fat diet, |
Online Access: | http://hdl.handle.net/10261/217922 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100008054 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100010198 |
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dig-iata-es-10261-2179222022-12-22T08:31:04Z NOD1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet González-Ramos, Silvia Paz-García, Marta Fernández-García, Victoria Portune, Kevin J. Acosta Medina, Emilio F. Sanz Herranz, Yolanda Castrillo, Antonio Martín-Sanz, Paloma Obregón, María Jesús Boscá, Lisardo Ministerio de Economía, Industria y Competitividad (España) European Commission Comunidad de Madrid Fundación Ramón Areces Instituto de Salud Carlos III Nucleotide-binding oligomerization domain protein Obesity High fat diet The contribution of the nucleotide-binding oligomerization domain protein NOD1 to obesity has been investigated in mice fed a high fat diet (HFD). Absence of NOD1 accelerates obesity as early as 2 weeks after feeding a HFD. The obesity was due to increases in abdominal and inguinal adipose tissues. Analysis of the resting energy expenditure showed an impaired function in NOD1-deficient animals, compatible with an alteration in thyroid hormone homeostasis. Interestingly, free thyroidal T4 increased in NOD1-deficient mice fed a HFD and the expression levels of UCP1 in brown adipose tissue were significantly lower in NOD1-deficient mice than in the wild type animals eating a HFD, thus contributing to the observed adiposity in NOD1-deficient mice. Feeding a HFD resulted in an alteration of the proinflammatory profile of these animals, with an increase in the infiltration of inflammatory cells in the liver and in the white adipose tissue, and an elevation of the circulating levels of TNF-α. In addition, alterations in the gut microbiota in NOD1-deficient mice correlate with increased vulnerability of their ecosystem to the HFD challenge and affect the immune-metabolic phenotype of obese mice. Together, the data are compatible with a protective function of NOD1 against low-grade inflammation and obesity under nutritional conditions enriched in saturated lipids. Moreover, one of the key players of this early obesity onset is a dysregulation in the metabolism and release of thyroid hormones leading to reduced energy expenditure, which represents a new role for these hormones in the metabolic actions controlled by NOD1. This work was supported by Grants SAF2017-82436R, AGL2017-88801-P and SAF2016-75004R from MINECO/AEI/FEDER/EU, S2017/BMD-3686 from Comunidad de Madrid, CIVP18A3864 from Fundación Ramón Areces and CIBERCV and CIBERHED (funded by the Instituto de Salud Carlos III) and Fondos FEDER. Peer reviewed 2020-08-13T10:28:57Z 2020-08-13T10:28:57Z 2020-07-23 artículo http://purl.org/coar/resource_type/c_6501 Scientific Reports 10: 12317 (2020) http://hdl.handle.net/10261/217922 10.1038/s41598-020-69295-2 2045-2322 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100008054 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100010198 32704052 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-82436-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-88801-P info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75004-R AGL2017-88801-P/AEI/10.13039/501100011033 S2017/BMD-3686/CIFRA2-CM Publisher's version https://doi.org/10.1038/s41598-020-69295-2 Sí open Springer Nature |
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Nucleotide-binding oligomerization domain protein Obesity High fat diet Nucleotide-binding oligomerization domain protein Obesity High fat diet |
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Nucleotide-binding oligomerization domain protein Obesity High fat diet Nucleotide-binding oligomerization domain protein Obesity High fat diet González-Ramos, Silvia Paz-García, Marta Fernández-García, Victoria Portune, Kevin J. Acosta Medina, Emilio F. Sanz Herranz, Yolanda Castrillo, Antonio Martín-Sanz, Paloma Obregón, María Jesús Boscá, Lisardo NOD1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet |
description |
The contribution of the nucleotide-binding oligomerization domain protein NOD1 to obesity has been investigated in mice fed a high fat diet (HFD). Absence of NOD1 accelerates obesity as early as 2 weeks after feeding a HFD. The obesity was due to increases in abdominal and inguinal adipose tissues. Analysis of the resting energy expenditure showed an impaired function in NOD1-deficient animals, compatible with an alteration in thyroid hormone homeostasis. Interestingly, free thyroidal T4 increased in NOD1-deficient mice fed a HFD and the expression levels of UCP1 in brown adipose tissue were significantly lower in NOD1-deficient mice than in the wild type animals eating a HFD, thus contributing to the observed adiposity in NOD1-deficient mice. Feeding a HFD resulted in an alteration of the proinflammatory profile of these animals, with an increase in the infiltration of inflammatory cells in the liver and in the white adipose tissue, and an elevation of the circulating levels of TNF-α. In addition, alterations in the gut microbiota in NOD1-deficient mice correlate with increased vulnerability of their ecosystem to the HFD challenge and affect the immune-metabolic phenotype of obese mice. Together, the data are compatible with a protective function of NOD1 against low-grade inflammation and obesity under nutritional conditions enriched in saturated lipids. Moreover, one of the key players of this early obesity onset is a dysregulation in the metabolism and release of thyroid hormones leading to reduced energy expenditure, which represents a new role for these hormones in the metabolic actions controlled by NOD1. |
author2 |
Ministerio de Economía, Industria y Competitividad (España) |
author_facet |
Ministerio de Economía, Industria y Competitividad (España) González-Ramos, Silvia Paz-García, Marta Fernández-García, Victoria Portune, Kevin J. Acosta Medina, Emilio F. Sanz Herranz, Yolanda Castrillo, Antonio Martín-Sanz, Paloma Obregón, María Jesús Boscá, Lisardo |
format |
artículo |
topic_facet |
Nucleotide-binding oligomerization domain protein Obesity High fat diet |
author |
González-Ramos, Silvia Paz-García, Marta Fernández-García, Victoria Portune, Kevin J. Acosta Medina, Emilio F. Sanz Herranz, Yolanda Castrillo, Antonio Martín-Sanz, Paloma Obregón, María Jesús Boscá, Lisardo |
author_sort |
González-Ramos, Silvia |
title |
NOD1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet |
title_short |
NOD1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet |
title_full |
NOD1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet |
title_fullStr |
NOD1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet |
title_full_unstemmed |
NOD1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet |
title_sort |
nod1 deficiency promotes an imbalance of thyroid hormones and microbiota homeostasis in mice fed high fat diet |
publisher |
Springer Nature |
publishDate |
2020-07-23 |
url |
http://hdl.handle.net/10261/217922 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100008054 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100010198 |
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