Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota
Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition. The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides. We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota. LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum. The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN. There was a significant reduction of Blautia coccoides group with LNB and 3FN. In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members. Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures. Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified. The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
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Format: | artículo biblioteca |
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Springer Nature
2020-07-16
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Subjects: | Human milk oligosaccharides, Gut microbiota, Prebiotics, Lacto-N-biose, Galacto-N-biose, Fucosyl-α1,3-GlcNAc, Fucosyl-α1,6-GlcNAc, |
Online Access: | http://hdl.handle.net/10261/217912 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 |
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dig-iata-es-10261-2179122021-12-27T16:42:17Z Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota Rubio-del-Campo, Antonio Alcántara, Cristina Collado, María Carmen Rodríguez-Díaz, Jesús Yebra, María Jesús Ministerio de Economía, Industria y Competitividad (España) European Commission Human milk oligosaccharides Gut microbiota Prebiotics Lacto-N-biose Galacto-N-biose Fucosyl-α1,3-GlcNAc Fucosyl-α1,6-GlcNAc Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition. The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides. We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota. LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum. The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN. There was a significant reduction of Blautia coccoides group with LNB and 3FN. In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members. Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures. Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified. The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods. This work was supported by the Spanish Ministry for Economy and Competitiveness (MINECO)/FEDER through the Project AGL2017-84165-C2 (1-R and 2-R). M.C.C. would like to acknowledge the support from European Research Council under the European Union's Horizon 2020 research and innovation programme (ERC starting grant, n° 639226) Peer reviewed 2020-08-13T09:38:06Z 2020-08-13T09:38:06Z 2020-07-16 artículo http://purl.org/coar/resource_type/c_6501 Scientific Reports 10: 1184 (2020) 2045-2322 http://hdl.handle.net/10261/217912 10.1038/s41598-020-68718-4 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 32678209 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84165-C2-1-R info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84165-C2-2-R info:eu-repo/grantAgreement/EC/H2020/639226 Publisher's version https://doi.org/10.1038/s41598-020-68718-4 Sí open Springer Nature |
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Human milk oligosaccharides Gut microbiota Prebiotics Lacto-N-biose Galacto-N-biose Fucosyl-α1,3-GlcNAc Fucosyl-α1,6-GlcNAc Human milk oligosaccharides Gut microbiota Prebiotics Lacto-N-biose Galacto-N-biose Fucosyl-α1,3-GlcNAc Fucosyl-α1,6-GlcNAc |
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Human milk oligosaccharides Gut microbiota Prebiotics Lacto-N-biose Galacto-N-biose Fucosyl-α1,3-GlcNAc Fucosyl-α1,6-GlcNAc Human milk oligosaccharides Gut microbiota Prebiotics Lacto-N-biose Galacto-N-biose Fucosyl-α1,3-GlcNAc Fucosyl-α1,6-GlcNAc Rubio-del-Campo, Antonio Alcántara, Cristina Collado, María Carmen Rodríguez-Díaz, Jesús Yebra, María Jesús Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota |
description |
Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition. The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides. We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota. LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum. The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN. There was a significant reduction of Blautia coccoides group with LNB and 3FN. In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members. Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures. Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified. The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods. |
author2 |
Ministerio de Economía, Industria y Competitividad (España) |
author_facet |
Ministerio de Economía, Industria y Competitividad (España) Rubio-del-Campo, Antonio Alcántara, Cristina Collado, María Carmen Rodríguez-Díaz, Jesús Yebra, María Jesús |
format |
artículo |
topic_facet |
Human milk oligosaccharides Gut microbiota Prebiotics Lacto-N-biose Galacto-N-biose Fucosyl-α1,3-GlcNAc Fucosyl-α1,6-GlcNAc |
author |
Rubio-del-Campo, Antonio Alcántara, Cristina Collado, María Carmen Rodríguez-Díaz, Jesús Yebra, María Jesús |
author_sort |
Rubio-del-Campo, Antonio |
title |
Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota |
title_short |
Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota |
title_full |
Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota |
title_fullStr |
Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota |
title_full_unstemmed |
Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota |
title_sort |
human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota |
publisher |
Springer Nature |
publishDate |
2020-07-16 |
url |
http://hdl.handle.net/10261/217912 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 |
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