SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area

SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area

Postprint disponible en http://hdl.handle.net/10261/211707

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Main Authors: Randazzo, Walter, Truchado, Pilar, Cuevas Ferrando, Enric, Simón, Pedro, Allende, Ana, Sánchez Moragas, Gloria
Other Authors: Consejo Superior de Investigaciones Científicas (España)
Format: artículo biblioteca
Language:English
Published: MedRxiv 2020-04-28
Online Access:http://hdl.handle.net/10261/209125
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100003359
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spelling dig-iata-es-10261-2091252020-10-20T12:38:34Z SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area Randazzo, Walter Truchado, Pilar Cuevas Ferrando, Enric Simón, Pedro Allende, Ana Sánchez Moragas, Gloria Consejo Superior de Investigaciones Científicas (España) Generalitat Valenciana Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Postprint disponible en http://hdl.handle.net/10261/211707 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 200,000 reported COVID-19 cases in Spain resulting in more than 20,800 deaths as of April 21, 2020. Faecal shedding of SARS-CoV-2 RNA from COVID-19 patients has extensively been reported. Therefore, we investigated the occurrence of SARS-CoV-2 RNA in six wastewater treatments plants (WWTPs) serving the major municipalities within the Region of Murcia (Spain), a low prevalence area. Firstly, an aluminum hydroxide adsorption-precipitation concentration method was tested using a porcine coronavirus (Porcine Epidemic Diarrhea Virus, PEDV) and mengovirus (MgV). The procedure resulted in average recoveries of 10.90% and 10.85% in influent water and 3.29% and 6.19% in effluent water samples for PEDV and MgV, respectively. Then, the method was used to monitor the occurrence of SARS-CoV-2 from March 12 to April 14, 2020 in influent, secondary and tertiary effluent water samples. By using the real-time RT-PCR (RT-qPCR) Diagnostic Panel validated by US CDC that targets three regions of the virus nucleocapsid (N) gene, we estimated quantification of SARS-CoV-2 RNA titers in untreated wastewater waters of 5.29 log genomic copies/L on average. Moreover, we tested as negative all secondary and tertiary treated water samples, highlighting that current water disinfection treatments applied in the analyzed WWTP are able to remove SARS-CoV-2 RNA. This environmental surveillance data were compared to declared COVID-19 cases at municipality level, revealing that SARS-CoV-2 was circulating among the population even before the first cases were reported by local or national authorities in many of the cities where wastewaters have been sampled. The detection of SARS-CoV-2 in wastewater in early stages of the spread of COVID-19 highlights the relevance of this strategy as an early indicator of the infection within a specific population. At this point, this environmental surveillance could be implemented by municipalities right away as a tool, designed to help authorities to coordinate the exit strategy to gradually lift its coronavirus lockdown. The study was funded by the projects 20180705 of ESAMUR and 202070E101 of CSIC. WR is supported by APOSTD/2018/150 postdoctoral fellowship and EC-F by APOTIP/2018/007 PhD student grant, both funded by the Generalitat Valenciana. PT is holder of the RYC2018- 025510-I Ramón y Cajal contract from the MICIU. No 2020-04-27T07:03:25Z 2020-04-27T07:03:25Z 2020-04-28 artículo http://purl.org/coar/resource_type/c_6501 http://hdl.handle.net/10261/209125 10.1101/2020.04.22.20075200 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003359 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC-2018-025510-I RYC-2018-025510-I/AEI/10.13039/501100011033 Preprint https://doi.org/10.1101/2020.04.22.20075200 Sí open MedRxiv
institution IATA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-iata-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IATA España
language English
description Postprint disponible en http://hdl.handle.net/10261/211707
author2 Consejo Superior de Investigaciones Científicas (España)
author_facet Consejo Superior de Investigaciones Científicas (España)
Randazzo, Walter
Truchado, Pilar
Cuevas Ferrando, Enric
Simón, Pedro
Allende, Ana
Sánchez Moragas, Gloria
format artículo
author Randazzo, Walter
Truchado, Pilar
Cuevas Ferrando, Enric
Simón, Pedro
Allende, Ana
Sánchez Moragas, Gloria
spellingShingle Randazzo, Walter
Truchado, Pilar
Cuevas Ferrando, Enric
Simón, Pedro
Allende, Ana
Sánchez Moragas, Gloria
SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area
author_sort Randazzo, Walter
title SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area
title_short SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area
title_full SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area
title_fullStr SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area
title_full_unstemmed SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area
title_sort sars-cov-2 rna titers in wastewater anticipated covid-19 occurrence in a low prevalence area
publisher MedRxiv
publishDate 2020-04-28
url http://hdl.handle.net/10261/209125
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100003359
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