Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)

Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)

© 2020 by the authors.

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Main Authors: Santiso-Bellón, Cristina, Randazzo, Walter, Pérez Cataluña, Alba, Vila-Vicent, Susana, Gozalbo-Rovira, Roberto, Muñoz, Carlos, Buesa, Javier, Sánchez Moragas, Gloria, Rodríguez Díaz, Jesús
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Subjects:Rotavirus, Norovirus, Sewage, Genotyping,
Online Access:http://hdl.handle.net/10261/205605
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100003359
http://dx.doi.org/10.13039/501100003329
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spelling dig-iata-es-10261-2056052022-06-24T09:40:10Z Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain) Santiso-Bellón, Cristina Randazzo, Walter Pérez Cataluña, Alba Vila-Vicent, Susana Gozalbo-Rovira, Roberto Muñoz, Carlos Buesa, Javier Sánchez Moragas, Gloria Rodríguez Díaz, Jesús Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Generalitat Valenciana Rotavirus Norovirus Sewage Genotyping © 2020 by the authors. The aim of the present study was to perform the molecular epidemiology of rotaviruses and noroviruses detected in sewage samples from a large wastewater facility from the city of Valencia, Spain. A total of 46 sewage samples were collected over a one-year period (September 2016 to September 2017). Norovirus and rotavirus were detected and quantified by RT-qPCR, genotyped by semi-nested RT-PCR and further characterized by sequencing and phylogenetic analyses. Noroviruses and rotaviruses were widely distributed in sewage samples (69.6% for norovirus GI, 76.0% norovirus GII, and 71.7% rotaviruses) and viral loads varied from 4.33 to 5.75 log PCRU/L for norovirus GI, 4.69 to 6.95 log PCRU/L for norovirus GII, and 4.08 to 6.92 log PCRU/L for rotavirus. Overall, 87.5% (28/32) of GI noroviruses could not be genotyped, 6.25% (2/32) of the samples contained GI.2 genotype, and another 6.25% (2/32) were positive for GI.4 genotype. The most common genotype of GII noroviruses was GII.2 (40%, 14/35), followed by GII.6 (8.6%, 3/35) and GII.17 (5.7%, 2/35) while the remaining GII strains could not be typed (45.7%, 16/35). Rotavirus VP4 genotype P[8] was the only one found in 19 out of 33 rotavirus-positive samples (57.7%). G2 was the most prevalent rotavirus VP7 genotype (15.2%, 5/33) followed by G3, G9, and G12, with two positive samples for each genotype (6.1%, 2/33). In one sample both G1 and G2 genotypes were detected simultaneously (3%). The results presented here show that the surveillance of noroviruses and rotaviruses in sewage is useful for the study of their transmission in the population and their molecular epidemiology. This work was supported by Spanish Government (Ministerio de Economía y Competitividad) grants AGL2017-84165-C2-2-R, RYC-2013-12442, and AGL2017-82909-R (AEI/FEDER, UE) funded by Spanish Ministry of Science, Innovation and Universities. This work was also founded by the Generalitat Valenciana and the UE FEDER program IDIFER/2018/056. CSB is the recipient of a predoctoral grant FPI from the Spanish Government RE2018-083315. RGR and WR are supported by postdoctoral fellowships from Valencian Government (APOST/2017/037 and APOSTD/2018/150). Peer reviewed 2020-03-27T13:25:27Z 2020-03-27T13:25:27Z 2020 2020-03-27T13:25:27Z artículo http://purl.org/coar/resource_type/c_6501 Microorganisms 8(3): 458 (2020) http://hdl.handle.net/10261/205605 10.3390/microorganisms8030458 2076-2607 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 32213877 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84165-C2-2-R AGL2017-84165-C2-2-R/AEI/10.13039/501100011033 info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2013-12442 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-82909-R AGL2017-82909-R/AEI/10.13039/501100011033 Publisher’s version https://doi.org/10.3390/microorganisms8030458 Sí open Multidisciplinary Digital Publishing Institute
institution IATA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-iata-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IATA España
language English
topic Rotavirus
Norovirus
Sewage
Genotyping
Rotavirus
Norovirus
Sewage
Genotyping
spellingShingle Rotavirus
Norovirus
Sewage
Genotyping
Rotavirus
Norovirus
Sewage
Genotyping
Santiso-Bellón, Cristina
Randazzo, Walter
Pérez Cataluña, Alba
Vila-Vicent, Susana
Gozalbo-Rovira, Roberto
Muñoz, Carlos
Buesa, Javier
Sánchez Moragas, Gloria
Rodríguez Díaz, Jesús
Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)
description © 2020 by the authors.
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Santiso-Bellón, Cristina
Randazzo, Walter
Pérez Cataluña, Alba
Vila-Vicent, Susana
Gozalbo-Rovira, Roberto
Muñoz, Carlos
Buesa, Javier
Sánchez Moragas, Gloria
Rodríguez Díaz, Jesús
format artículo
topic_facet Rotavirus
Norovirus
Sewage
Genotyping
author Santiso-Bellón, Cristina
Randazzo, Walter
Pérez Cataluña, Alba
Vila-Vicent, Susana
Gozalbo-Rovira, Roberto
Muñoz, Carlos
Buesa, Javier
Sánchez Moragas, Gloria
Rodríguez Díaz, Jesús
author_sort Santiso-Bellón, Cristina
title Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)
title_short Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)
title_full Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)
title_fullStr Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)
title_full_unstemmed Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)
title_sort epidemiological surveillance of norovirus and rotavirus in sewage (2016–2017) in valencia (spain)
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://hdl.handle.net/10261/205605
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100003359
http://dx.doi.org/10.13039/501100003329
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